Abstract
Introduction
As water transporters, aquaporins (AQPs) are closely related to other membrane transporters, and water permeability in cell may contribute to chemosensitivity of tumor. To understand the correlation between AQPs and cisplatin (DDP) sensitivity to ovarian cancer cells, effects of DDP on AQPs expression were detected in vitro, and chemosensitivity of DDP was observed in hypertoncity in vitro.
Methods
SKOV3 cells were incubated with DDP, aquaporins blocker mercuric chloride, or in hypertonicity, and cell proliferation inbihition was measured by MTT. Effects of DDP on AQPs mRNA expression cancer cell SKOV3 were measured by RT-PCR.
Results
MTT analyses showed that aquaporin blocker and hypertonicity increased the sensitivity of SKOV3 to DDP. The effects of DDP on AQPs expression were different between aquaporin subtypes: following an increase in the incubation time, the expression of AQP1 mRNA decreased significantly, but expression of AQP3 and AQP8 increased.
Conclusion
Our studies have showed that different subtypes of AQPs play different roles in ovarian cancer cell in vitro, and which suggested that AQPs might be associated with chemotherapy sensitivity of ovarian cancer.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Verkman AS, Hara-Chikuma M (2008) Aquaporins––new players in cancer biology. J Mol Med 86:523–529
Mccoy E, Sontheimer H (2007) Expression and function of water channels (aquaporins) in migration malignant astrocytes. Glia 55:1034–1043
Chae YK, Woo J, Kim M-J et al (2008) Expression of aquaporin 5 promotes tumor invasion in human non-small cell lung cancer. Plos One 5(2162):1–9
Yang JH, Shi YF, Cheng Q (2006) Expression and localization of aquaporin-5 in the epithelial ovarian tumor. Gynecol Oncol 100(2):294–299
Yang JH, Shi YF, Chen XD, Qi WJ (2006) The influence of aquaporin-1 and microvessel density on ovarian carcinogenesis and ascites formation. Int J Gynecol Cancer 16(Suppl.):400–405
Yang JH, Yu YQ, Yan CX (2011) Localisation and expression of aquaporin subtypes in epithelial ovarian tumours. Histol Histopathol 26(9):1197–1205
Yang JH, Yan CX, Chen XJ, Zhu YS (2011) Expression of aquaglyceroporins in epithelial ovarian tumors and their clinical significance. J Int Med Res 39(3):702–711
Bhattacharjee H, Rosen BP, Mukhopadhyay R (2009) Aquaglyceroporins and metalloid transport: implication in human diseases. Handb Exp Pharmacol 190:309–325
Leung J, Pang A, Yuen W-H et al (2007) Relationship of expression of aquaglycer 9 with arsenic uptake and sensitivity in leukemia cells. Blood 109:740–746
Naranmandura H, Ogra Y, Iwata K (2009) Evidence for toxicity differences between inorganic arsenite and thioarsenicals in human bladder cancer cells. Toxicol Appl Pharmacol 238(2):133–140
Elizabeth M, Jablonski M, Mattocks A, Sololov E et al (2007) Decreased aquaporin expression leads to increased resistance to apoptosis in hepatocellular carcinoma. Cancer Lett 250(1):36–46
Yang J, Yan C, Zheng W, Chen X (2012) Proliferation inhibition of cisplatin and aquaporin 5 expressions in human ovarian cancer cell CAOV3. Arch Gynecol Obstet 285(1):239–245 (Epub 2011 May 26)
Moon C, Rousseau R, Soria J-C et al (2004) Aquaporin expression in human lymphocytes and dendritic cells. Am J Hematol 75:128–133
Wang HF, Liu Y, Qu X et al (2007) The expression of AQP1-10 mRNA in the human mesothelial cells. Prog Biochem Biophys 43(1):57–62
Savage DF, Stroud RM (2007) Structural basis of aquaporin inhibition by mercury. J Mol Biol 368:607–617
Gottesman MM, Fojo T, Bates SE (2002) Multidrug resistance in cancer: role of ATP-dependent transporters. Nat Rev Cancer 2:48–58
He L, Vasiliou K, Nebert DW (2009) Analysis and update of the human solute carrier (SLC) gene superfamily. Hum Genomics 3(2):195–206
Huang Y, Sadee W (2006) Membrane transporters and channels in chemoresistance and sensitivity of tumors cells. Cancer Lett 239:168–182
Umenishi F, Schrier RW (2003) Hypertonicity-induced aquaporin-1 (AQP1) expression is mediated by the activation of MAPK pathways and hypertonicity-responsive element in the AQP1 gene. J Biol Chem 287(18):15765–15770
Umenishi F, Schrier RW (2002) Idengifacation and characterization of a novel hypertonicity-responsive element in the human aquaporin-1 gene. Biochem Biophys Res Commun 292:771–775
Matsuzaki T, Suzuki T, Takata K (2001) Hypertonicity-induced expression of aquaporin 3 in MDCK cells. Am J Physiol Cell Physiol 281(1):C55–C63
Pedersen PS, Braunstein TH, Jorhensen A et al (2007) Stimulation of aquaporin-5 and trans-epithelial water permeability in human airway epithelium by hyperosmotic stress. Pfluger Arch 453(6):75–777
Arima H, Yamamoto N, Sobue K et al (2003) Hyperosmolar mannitol stimulates expression of aquaporin 4 and 9 through a p38 mitogen-activated protein kinase-dependent pathway in rat astrocytes. J Biol Chem 278(45):44525–44534
Acknowledgments
This study was supported by Grants from the National Natural Science Foundation of China (Grant 81202064), and the Research Fund for Zhejiang provincial natural science foundation (Grant Y2110467).
Conflict of interest
The authors had no conflict of interest to declare in relation to this article.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Xuejun, C., Weimin, C., Xiaoyan, D. et al. Effects of aquaporins on chemosensitivity to cisplatin in ovarian cancer cells. Arch Gynecol Obstet 290, 525–532 (2014). https://doi.org/10.1007/s00404-014-3216-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00404-014-3216-6