Abstract
Aims
The purpose of this study is to investigate the association between proliferation inhibition of cisplatin and aquaporin 5 (AQP5) expression and its regulation in ovarian carcinoma cell CAOV3.
Methods
Cell growth rate was measured by MTT after CAOV3 cells were incubated with cisplatin or NF-κB inhibitor PDTC. Western blot and RT-PCR were used to detect the expression of AQP5 and NF-κB p65.
Results
Our results showed that expression of AQP5, NF-κB in cytoplasm and karyon and IκBα in cytoplasm protein in CAOV3 cells can be induced to decrease by cisplatin with concentration-dependent manner, and there is a positive correlation between AQP5 protein and cell growth rate (r = 0.607, P < 0.05). When cells were incubated with 10 μg/ml cisplatin, AQP5, NF-κB p65, and IκBα increased rapidly at 6–12 h, but decreased at 24 h, remain on low level until to 72 h. Expression of AQP5 could be induced to decrease by PDTC, and a positive correlation between AQP5 protein expression and NF-κB p65 and IκBα (r = 0.894, 0.857; P < 0.05).
Conclusions
Proliferation inhibition of cisplatin is related with AQP5 expression, and NF-κB may be involved in mechanism of AQP5 regulation. AQP5 will be potential target for therapy of ovarian carcinoma.
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Acknowledgments
This study was supported by a grant of National Natural Science Foundation of China (Grant number: 200904435047), and Research Fund for the Doctoral Program of Higher Education of China (Grant number: 20070335047).
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We declare that we have no conflict of interest.
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Yang, J., Yan, C., Zheng, W. et al. Proliferation inhibition of cisplatin and aquaporin 5 expression in human ovarian cancer cell CAOV3. Arch Gynecol Obstet 285, 239–245 (2012). https://doi.org/10.1007/s00404-011-1908-8
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DOI: https://doi.org/10.1007/s00404-011-1908-8