Abstract
The role of dendritic cells in vitiligo is still unclear. Few studies have provided contradictory results about their quantitative variation and no data exist concerning their immunophenotypical distribution in diseased skin. The purpose of our study was to analyze the presence, the distribution, the immunophenotypical markers and the effects of nb-UVB therapy on dendritic cells in non-lesional, perilesional, and lesional vitiligo skin. Punch-biopsies of 6 mm were taken from lesional, perilesional, and non-lesional skin of 12 patients affected by non-segmental vitiligo, treated with nb-UVB. An immunohistochemical and an ultrastructural analysis were performed. Immunohistochemical and ultrastructural analysis showed both quantitative and qualitative modifications of Langerhans cells. Nb-UVB therapy, one of the most effective treatments for the disease, was able to reduce the Langerhans cells number and to redistribute main dendritic subsets. This study underlines the importance of dendritic cells, Langerhans cells in particular, in non-segmental vitiligo, in its pathogenesis and in its better therapeutical approach.
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Abdulla SJ, Desgroseilliers JP (2008) Treatment of vitiligo with narrow-band ultraviolet B: advantages and disadvantages. J Cutan Med Surg 12(4):174–179
Becatti M, Prignano F, Fiorillo C et al (2010) The involvement of Smac/DIABLO, p53, NF-kB, and MAPK pathways in apoptosis of keratinocytes from perilesional vitiligo skin: protective effects of curcumin and capsaicin. Antioxid Redox Signal 13(9):1309–1321
Bedoui S, Whitney PG, Waithman J et al (2009) Cross-presentation of viral and self antigens by skin-derived CD103+ dendritic cells. Nat Immunol 10(5):488–495
Dammak I, Boudaya S, Abdallah FB, Turki H, Attia H, Hentati B (2009) Antioxidant enzymes and lipid peroxidation at the tissue level in patients with stable and active vitiligo. Int J Dermatol 48:476–480
Fitzpatrick TB, Breathnach AS (1963) Die epidermale Melanin-Einheit-system. Dermatol Woechenschr 147:635–642
Grimes PE (2004) White patches and bruised souls: advances in the pathogenesis and treatment of vitiligo. J Am Acad Dermatol 51:85–87
Hautmann G, Moretti S, Lotti T, Hercogovà J (2004) Pathogenesis of vitiligo: evidence for a possible ongoing disorder of the cutaneous microenvironment. In: Lotti T, Hercogovà J (eds) Vitiligo: new data and hypotheses. Marcel-Dekker, New York, pp 99–121
Huggings RH, Schwartz RA, Janniger CK (2005) Vitiligo. Acta Dermatoven APA 14(4):137–142, 144–145
Le Poole IC, Das PK, van den Wijngaard RMJGJ et al (1993) Review of the etiopathomecanism of vitiligo: a convergence theory. Exp Dermatol 2:145–153
Lotti T, Berti S, Moretti S (2009) Vitiligo therapy. Expert Opin Pharmacother 10(17):2779–2785
Lotti T, Prignano F, Buggiani G (2007) New and experimental treatments of vitiligo and other hypomelanoses. Dermatol Clin 25:393–400
Montes LF, Abulafia J, Wilborn WH et al (2003) Value of histopathology in vitiligo. Int J Dermatol 42:57–61
Ongenae K, Van Geel N, Naeyaert JM (2003) Evidence for an autoimmune pathogenesis of vitiligo. Pigment Cell Res 16:90–100
Panuncio AL, Vignale R (2003) Ultrastructural studies in stable vitiligo. Am J Dermatopathol 25(1):16–20
Pradhan S, Kyung Kim H, Thrash CJ et al (2008) A critical role for the proapoptic protein Bid in ultraviolet-induced immune suppression and cutaneous apoptosis. J Immunol 181:3077–3088
Prignano F, Domenici L, Carli P et al (1999) Langerhans cell Histiocytosis of the vulva: an ultrastructural study. Ultrastruct Pathol 23:127–132
Prignano F, Pescitelli L, Becatti M et al (2009) Ultrastructural and functional alterations of mitochondria in perilesional vitiligo skin. J Dermatol Sci 54:157–167
Prignano F, Ricceri F, Bianchi B et al (2009) Quantity, distribution and immunophenotypical modification of dendritic cells upon biological treatments in psoriasis. Int J Immunopathol Pharmacol 22(2):379–387
Rattis FM, Concha M, Delbiez-Gauthier C et al (1998) Effects of Ultraviolet B radiation on human Langerhans cells: functional alteration of CD86 upregulation and induction of apoptotic cell death. J Invest Dermatol 3:373–379
Taieb A, Picardo M (2007) The definition and assessment of vitiligo: a consensus report of vitiligo European Task Force. Pigment Cell Res 20:27–35
Weiss JM, Renkl AC, Denfeld RW et al (1995) Low-dose UVB radiation perturbs the functional expression of B7.1 and B7.2 costimulatory molecules on human Langerhans cells. Eur J Immunol 25:2858–2862
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Prignano, F., Ricceri, F., Bianchi, B. et al. Dendritic cells: ultrastructural and immunophenotypical changes upon nb-UVB in vitiligo skin. Arch Dermatol Res 303, 231–238 (2011). https://doi.org/10.1007/s00403-010-1109-5
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DOI: https://doi.org/10.1007/s00403-010-1109-5