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Modulation of integrins and filaggrin expression by Propionibacterium acnes extracts on keratinocytes

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Abstract

Propionibacterium acnes plays an important role in the pathogenesis of acne and it is established that this bacteria is involved in the induction and maintenance of the inflammatory phase of acne. The aim of our work was to determine if P. acnes extracts could modulate integrins and filaggrin in vitro expression by keratinocytes. Integrins and filaggrin expression was examined using immunohistochemistry technique both on Normal Human Epiderminal Keratinocytes (NHEK) and on deep-frozen sections of normal human skin explants incubated with three different P. acnes extracts. In addition, the expression of filaggrin was investigated on biopsies of acne lesions and by western-blot associated with its precursor profilaggrin. We demonstrated that P. acnes extracts induced β1 integrin expression significantly on both proliferating keratinocytes and differentiated keratinocytes. In addition, P. acnes induced α3, α6s and αVβ6 integrin expression and filaggrin expression on differentiated keratinocytes. Finally P. acnes extracts increased filaggrin expression by suprabasal layer of epidermis of explants. Western-blot confirmed that total amount of filaggrin was increased. These results indicate that P. acnes extracts are directly able to modulate the differentiation of keratinocytes suggesting that this bacteria play a role not only in the development of inflammatory acne lesions but also in the formation of the microcomedo.

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Acknowledgments

Financial support and P. acnes extracts were provided by Pierre Fabre Laboratories (Toulouse, France). No conflicts of interest exist. We thank surgeons from the plastic surgery Department of Nantes Hospital for abdominal skin samples and the pediatric surgery Department of Nantes Hospital for foreskin samples. The authors thank Simon M. (Toulouse) for antibodies AHF2 and AHF7 gift.

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Authors and Affiliations

  1. INSERM U601, 9 quai Moncousu, 44093, Nantes Cedex 01, France

    V. Jarrousse, A. Khammari & B. Dréno

  2. Clinique Dermatologique, Chu Hôtel-Dieu, 1 Place Alexis Ricordeau, 44000, Nantes, France

    A. Khammari & B. Dréno

  3. Laboratoire de Biologie Cellulaire Cutanée, Institut de Recherche Pierre Fabre, Toulouse, France

    N. Castex-Rizzi & M. Charveron

  4. Department of Dermatology, Chu Hotel Dieu, Place Alexis Ricordeau, 44035, Nantes Cedex 01, France

    B. Dréno

Authors
  1. V. Jarrousse
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  2. N. Castex-Rizzi
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  3. A. Khammari
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  4. M. Charveron
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  5. B. Dréno
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Correspondence to B. Dréno.

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Jarrousse, V., Castex-Rizzi, N., Khammari, A. et al. Modulation of integrins and filaggrin expression by Propionibacterium acnes extracts on keratinocytes. Arch Dermatol Res 299, 441–447 (2007). https://doi.org/10.1007/s00403-007-0774-5

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  • DOI: https://doi.org/10.1007/s00403-007-0774-5

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