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Mosaicplasty associated with gene enhanced tissue engineering for the treatment of acute osteochondral defects in a goat model

  • Orthopaedic Surgery
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Abstract

Objective

To compare single mosaicplasty, mosaicplasty associated with gene enhanced tissue engineering and mosaicplasty associated with the gels of non-gene transduced bone mesenchymal stem cells (BMSCs) in alginate for the treatment of acute osteochondral defects in a goat model.

Methods

The principle and methods of tissue engineering were used. BMSCs were separated and amplified in vitro, and human transforming growth factor-β1 (hTGF-β1) gene was transduced to the cells. Then, the cells were suspended in the alginate. At the same time using mosaicplasty to repair the defects on the medial femoral condyle, the dead space between the cylindrical grafts were filled with the gels of hTGF-β1 gene transduced BMSCs in alginate. Single mosaicplasty and mosaicplasty associated with the gels of non-gene transduced BMSCs in alginate were compared by the different time observation.

Results

All of the three treatments could repair the acute osteochondral defects. Mosaicplasty associated with gene enhanced tissue engineering had a better integration than single mosaicplasty, and mosaicplasty associated with the gels of non-gene transduced BMSCs in alginate.

Conclusion

Mosaicplasty associated with tissue engineering could solve the problem of the poor concrescence of the remnant defect and the integration of single mosaicplasty.

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Acknowledgments

The authors like to thank Shanghai Puwei Orthopedics Ltd for the provision of supplies and surgical instruments, the orthopedic laboratory of Shanghai No. 9 Hospital affiliated to Shanghai Jiaotong University for the Adv-hTGF-β1, Adv-βgal and 293 cells. The corresponding author did the above research as he studied a PhD degree at Shanghai No. 9 Hospital affiliated to Shanghai Jiaotong University.

Conflict of interest statement

No benefits in any form have been received or will be received from a commercial party related directly or indirectly to the subject of this article.

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Authors and Affiliations

  1. Department of Orthopedics, Shanghai Shu Guang Hospital, Shanghai Traditional Chinese Medical University, Shanghai, China

    Jun Sun, Ru-Ming Zhang, Yong Kuang & Meng Shi

  2. Department of Orthopedics, Shanghai No. 9 Hospital, Shanghai Jiaotong University, Shanghai, China

    Xiao-Kui Hou, Xu Li & Ting-Ting Tang

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  1. Jun Sun
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Correspondence to Jun Sun.

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Sun, J., Hou, XK., Li, X. et al. Mosaicplasty associated with gene enhanced tissue engineering for the treatment of acute osteochondral defects in a goat model. Arch Orthop Trauma Surg 129, 757–771 (2009). https://doi.org/10.1007/s00402-008-0761-0

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  • DOI: https://doi.org/10.1007/s00402-008-0761-0

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