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Upregulation of type I interleukin−1 receptor after traumatic spinal cord injury in adult rats

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Abstract

Post-traumatic inflammation response has been implicated in secondary injury mechanisms after spinal cord injury (SCI). Interleukin-1 (IL-1) is a key inflammatory mediator that is increasingly expressed after SCI. The action of IL-1 is mediated through its functional receptor, type I interleukin-1 receptor (IL-1RI). However, whether this receptor is expressed after SCI remains to be elucidated. In the present study, the temporospatial expression of IL-1RI was detected in rats that received a moderate contusive SCI (a 10 g rod dropped at a height of 12.5 mm) at the ninth to tenth thoracic vertebral level using a widely used New York University impact device. Our study demonstrated that IL-1RI was slightly increased at 4 h post-injury compared to the normal or sham-operated controls, reached the peak at 8 h at mRNA level (4.44-fold, P<0.01) and 1 d at protein level (2.62-fold, P<0.01). IL-1RI remained at its elevated levels for a relatively long duration (4 h–7 days). Spatially, IL-1RI was observed throughout the entire length of a 10 mm-long cord segment containing the injury epicenter. Colocalization of IL-1RI was found in neurons, oligodendrocytes, astrocytes, and activated microglia. Our results suggest that the elevated expression of IL-1RI after SCI may contribute to posttraumatic inflammation responses of IL-1.

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Acknowledgments

This work was supported by the grant from Major State Basic Research Development Program of China (973 Project) (2003CB515302) and Shanghai Science Development Fund (02JC14014). The authors thank Zhen Guan, research associate from the Spinal Cord Injury Research Center, Ohio State University, for his technical assistance.

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Authors and Affiliations

  1. Department of Neurobiology, Shanghai Second Medical University, 280 South Chong-Qing Road, 200025, Shanghai, People’s Republic of China

    Xiao-Fei Wang, Li-Dong Huang, Pan-Pan Yu, Jian-Guo Hu, Lan Yin, Li Wang, Xiao-Ming Xu & Pei-Hua Lu

  2. Department of Laboratory Science, Affiliated Hospital of Nantong University, 226001, Nantong, People’s Republic of China

    Xiao-Fei Wang

  3. Kentucky Spinal Cord Injury Research Center, Departments of Neurological Surgery, University of Louisville School of Medicine, Louisville, KY, 40292, USA

    Xiao-Ming Xu

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  1. Xiao-Fei Wang
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Correspondence to Xiao-Ming Xu or Pei-Hua Lu.

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Wang, XF., Huang, LD., Yu, PP. et al. Upregulation of type I interleukin−1 receptor after traumatic spinal cord injury in adult rats. Acta Neuropathol 111, 220–228 (2006). https://doi.org/10.1007/s00401-005-0016-x

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