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An ultrosensitive biosensor based on electroactive nanoparticles self-assembled from 3-thiophenecarboxylic acid-modified starch

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Abstract

A kind of electroactive nanoparticles (NPs) was prepared in solution by self-assembling amphiphilic 3-thiophenecarboxylic acid-modified starch (TPCA-St), which was synthesized through esterification reaction. By casting TPCA-St NPs on the surface of gold electrode and subsequent electropolymerization of the thiophene moieties in NP film, the electroconductive TPCA-St NP-based film was formed. After horseradish peroxidase and Nafion were sequentially cast on the film surface, a biosensor was successfully prepared. The prepared biosensor showed high sensitivity for H2O2 detection. The linear range from 1 × 10−10 to 1 × 10−5 mol L−1 with a detection limit of 3 × 10−11 mol L−1 was obtained for H2O2 sensing. The biosensor also showed good repeatability and stability, and it has been successfully used to sense H2O2 in commercial disinfector with satisfactory results.

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Funding

This work was supported by the National Natural Science Foundation of China (NSFC 21504001, 51703001, 51572004), the foundation of Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, Jiangnan University (JDSJ2015-02).

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Authors and Affiliations

  1. School of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu, 241000, China

    Rongli Zhang, Zhenzhen Jin, Cuige Zhang, Renchun Yang & Mingyue Sun

  2. School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA, 30332, USA

    Rongli Zhang & Ching-Ping Wong

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  1. Rongli Zhang
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  2. Zhenzhen Jin
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  3. Cuige Zhang
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  4. Renchun Yang
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  5. Mingyue Sun
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  6. Ching-Ping Wong
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Correspondence to Rongli Zhang.

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The authors declare that they have no competing interests.

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Zhang, R., Jin, Z., Zhang, C. et al. An ultrosensitive biosensor based on electroactive nanoparticles self-assembled from 3-thiophenecarboxylic acid-modified starch. Colloid Polym Sci 296, 1365–1372 (2018). https://doi.org/10.1007/s00396-018-4359-y

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  • DOI: https://doi.org/10.1007/s00396-018-4359-y

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