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Adsorption and electrochemical behavior of Cyt-c on carbon nanotubes/TiO2 nanocomposite films fabricated at various annealing temperatures

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Abstract

Multi-walled carbon nanotubes (MWCNTs) were incorporated into the active layer of mesoporous TiO2 films resulting in MWCNTs-TiO2 nanocomposites with improved electrical conductivity. These MWCNTs-TiO2 nanocomposite films were prepared by a direct mixing method and the “doctor blade” technique. The films were sintered at various annealing temperatures (300, 350, 400, and 450 °C) in order to examine the effect of annealing temperature to the morphology and electrochemical activity of the films. The presence of anatase TiO2 and MWCNTs has been confirmed by X-ray diffraction (XRD), field emission scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Raman spectroscopy, while conductivity and electrochemical properties of the nanocomposite MWCNΤs-TiO2 films were examined via cyclic voltammetry (CV) and spectroelectrochemistry. After successful protein immobilization (Cyt-c), the electrochemical and spectroelectrochemical behavior of these hybrid electrodes (Cyt-c/MWCNTs-TiO2) was examined in detail and particularly the effect of MWCNTs on the interfacial electron transfer between the film electrode and the adsorbed protein molecules.

Schematic illustration of MWCNTs-TiO2 nanocomposite films used for Cyt-c immobilization.

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Acknowledgements

This work was partially supported by a small initiative grant from the University of Patras.

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Authors and Affiliations

  1. Department of Materials Science, University of Patras, 26504, Rio, Greece

    Emmanuel Topoglidis, Penthensileia-Amalia Kolozoff, Christina Tiflidis & Joan Papavasiliou

  2. Foundation for Research and Technology-Hellas (FORTH), Institute of Chemical Engineering Sciences (ICE-HT), P.O. Box 1414, GR-26504, Patras, Greece

    Joan Papavasiliou

  3. Institute of Nanoscience and Nanotechnology, National Center of Scientific Research Demokritos, Athens, Greece

    Elias Sakellis

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  1. Emmanuel Topoglidis
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  2. Penthensileia-Amalia Kolozoff
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  3. Christina Tiflidis
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Correspondence to Emmanuel Topoglidis.

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Topoglidis, E., Kolozoff, PA., Tiflidis, C. et al. Adsorption and electrochemical behavior of Cyt-c on carbon nanotubes/TiO2 nanocomposite films fabricated at various annealing temperatures. Colloid Polym Sci 296, 1353–1364 (2018). https://doi.org/10.1007/s00396-018-4358-z

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