Abstract
The effects of surfactants on protease with and without stabilizer boric acid were investigated in this paper. From the structural features of surfactants, we discussed the possible mechanism between surfactants and protease. Surfactants studied were anionic surfactants having varying hydrophilic group and nonionic surfactants having varying hydrophobic types and chain length. The results showed that anionic surfactant linear alkyl benzene sulfonate (LAS) has the biggest influence on protease, due to benzene ring in its structure; nonionic surfactant fatty alcohol polyoxyethylene (AEO) with more ethylene oxide shows bigger impact on protease; carbon chain length in alkyl polyglucosides (APGs) has less influence on protease. Understanding the reaction mechanism of surfactants and protease is essential for many industry uses. Especially the results provide references for the application of protease in laundry formulation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Geoffrey L, Russell, Larry N, Britton (2002) Use of certain alcohol ethoxylates to maintain protease stability in the presence of anionic surfactants. J Surfactant Deterg 1:5–9
Crutzen A, Douglass ML (1999) Detergent enzymes: a challenge! In: Handbook of detergents. Part a: properties, New York, pp 639–690
Steinhardt J, Retnolds JA (1969) Multiple equilibria in proteins. Academic Press, New York, p 234
Kravetz L, Guin KF (1985) Effect of surfactant structure on stability of enzymes formulated into laundry liquids. J Am Oil Chem Soc 62:943–949
Daniel E, Otzen (2002) Protein unfolding in detergents: effect of micelle structure, ionic strength, pH, and temperature. Biophys J 10:2219–2230
Qing-li Y, Xiu-quan Y (2005) Effects of some surfactants on activity of liquid protease. Appl Chem Ind 5:296–298
Lu G-Q, Zhang L-P (2010) Study on application of protease in liquid detergent product. Mod Chem Ind 11:124–127
Stoner MR, Douglas A et al (2004) Protease autolysis in heavy-duty liquid detergent formulations: effects of thermodynamic stabilizers and protease inhibitors. Enzym Microb Technol 34:114–125
Carrière F, Renou C, Ransac S, Lopez V, De Caro J, Ferrato F et al (2001) Inhibition of gastrointestinal lipolysis by orlistat during digestion of test meals in healthy volunteers. Am J Physiol Gastrointest Liver Physiol 281:16–28
Wang F-R, Pang Y-Z (1981) Folin phenol reagent method for test conditions of protease activity. Chin Acad J Electron Pub House, pp:21–24
J. Xia, X. Chen, I.A. Nnanna (1996) Activity and stability of penicillium cyclopium lipase in surfactant and detergent solutions. JAOCS Press, pp:115–120
Attwood D, Florence AT (1983) Surfactant systems: their chemistry, pharmacy, and biology, 1983rd edn. Chapman and Hall, New York, pp 630–631
Qing L, Nielsen VS (2002) A new stabilizer for in liquid detergents savinase ultra 16L/16XL. Deterg Cosmet 25:121–123
Acknowledgments
The financial support from the Natural Science Foundation of Shanxi Province (2013011040–4) and oversea returner technology support program of Shanxi Province (2012) is gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhang, J., Zhang, J. Study on the interaction of alkaline protease with main surfactants in detergent. Colloid Polym Sci 294, 247–255 (2016). https://doi.org/10.1007/s00396-015-3777-3
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00396-015-3777-3