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Decondensation of cationic gemini surfactant-induced DNA aggregates using triblock copolymer (PEO)20–(PPO)70–(PEO)20

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Abstract

Decondensation of DNA molecules, previously compacted by cationic gemini surfactant 12-3-12 · 2Br, has been successfully achieved by introducing triblock copolymer (PEO)20–(PPO)70–(PEO)20 (P123). P123 can interact with 12-3-12 · 2Br to form supramolecular assemblies through hydrophobic interactions, while not interacting with DNA. When introducing 12-3-12 · 2Br into P123/DNA system, the presence of P123 will inhibit the formation of DNA/12-3-12 · 2Br complexes due to the stronger interaction between P123 and 12-3-12 · 2Br. For previously formed DNA/12-3-12 · 2Br complexes, the addition of P123 can lead to the release of DNA from the complex, which should be attributed to the complexation of P123 with free 12-3-12 · 2Br surfactants in bulk phase followed by the breakup of the thermodynamic equilibrium between surfactant aggregates associated with DNA and free surfactants in bulk phase. CD experiments reveal that 12-3-12 · 2Br can change the conformation of DNA from typical B-form to ψ-phase by formation of DNA/12-3-12 · 2Br complexes. However, the release of the surfactant from the complex induced by P123 turns DNA conformation from ψ-phase back to B-form.

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Acknowledgments

This work is supported by the National Natural Science Foundation of China (Project No. 21173079), the 111 Project of Ministry of Education of China (No. B08021), and the Fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. Key Laboratory for Advanced Materials and Department of Chemistry, East China University of Science and Technology, Shanghai, 200237, China

    Yunfei He, Shouhong Xu, Di Sun, Yazhuo Shang, Xiaofang Zhao & Honglai Liu

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  1. Yunfei He
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  2. Shouhong Xu
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  3. Di Sun
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  4. Yazhuo Shang
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  5. Xiaofang Zhao
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Correspondence to Yazhuo Shang.

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He, Y., Xu, S., Sun, D. et al. Decondensation of cationic gemini surfactant-induced DNA aggregates using triblock copolymer (PEO)20–(PPO)70–(PEO)20 . Colloid Polym Sci 291, 2139–2146 (2013). https://doi.org/10.1007/s00396-013-2954-5

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  • DOI: https://doi.org/10.1007/s00396-013-2954-5

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