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Alginate beads containing pH-sensitive liposomes and glucose oxidase: glucose-sensitive release

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Abstract

Egg PC (EPC) liposomes bearing a copolymer of N-isopropylacrylamide, methacrylic acid, and octadecylacrylate (P(NIPAM-co-MAA-co-ODA)) were prepared as pH-sensitive liposomes. They were embedded in glucose oxidase (GOD)-immobilized alginate beads. The ratio of EPC/GOD/alginate in the beads was 7.8:1.0:140.4, and the beads were added to glucose solutions so that the concentration of GOD was 0.0068 mg/ml. The enzymatic activity of the immobilized GOD was one fifth to half of that of native enzyme. As the glucose concentration increased from 0 to 400 mg/dl, the degree of calcein release increased from 17% to 75%. The acidification induced by the enzymatic reaction would be responsible for the glucose-triggered release.

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References

  1. Horbett T, Kost J, Ratner B (1983) Swelling behavior of glucose sensitive membranes. Am Chem Soc, Div Polym Chem 24:34–35

    CAS  Google Scholar 

  2. Kost J, Horbett T, Ratner B, Singh M (1985) Glucose sensitive membranes containing glucose oxidase: activity, swelling and permeability studies. J Biomed Mater Res 19:1117–1133

    Article  CAS  Google Scholar 

  3. Albin G, Horbett T, Ratner B (1985) Glucose sensitive membranes for controlled delivery of insulin: insulin transport studies. J Control Release 2:153–164

    Article  CAS  Google Scholar 

  4. Albin G, Horbett T, Miller S, Ricker N (1987) Theoretical and experimental studies of glucose sensitive membranes. J Control Release 6:267–291

    Article  CAS  Google Scholar 

  5. Albin G, Horbett T, Ratner B (1990) Glucose-sensitive membranes for controlled release of insulin. In: Kost J (ed) Pulsed and self-regulated drug delivery. CRC, Boca Raton, pp 159–185

    Google Scholar 

  6. Jeong SY, Kim SW, Eenink MJD, Feijen J (1984) Self-regulating insulin delivery systems I. Synthesis and characterization of glycosylated insulin. J Control release 1:57–66

    Article  CAS  Google Scholar 

  7. Kim SW, Jeong SY, Sato S, McRea JC, Feijen J (1984) Self regulating insulin delivery system — a chemical approach. In: Anderson JM, Kim SW (eds) Recent advances in drug delivery systems. Plenum, New York, pp 123–136

    Google Scholar 

  8. Sato S, Jeong SY, McRea JC, Kim SW (1984) Self-regulating insulin delivery systems II. In vitro studies. J Control Release 1:67–77

    Article  CAS  Google Scholar 

  9. Sato S, Jeong SY, McRea JC, Kim SW (1984) Glucose stimulated insulin delivery system. Pure Appl Chem 56:1323–1328

    Article  CAS  Google Scholar 

  10. Jeong SY, Kim SW, Holemberg D, McRea JC (1985) Glucose sensitive membranes for controlled delivery of insulin: insulin transport studies. J Control Release 2:153–164

    Article  Google Scholar 

  11. Seminoff L, Kim SW (1990) A self- regulating insulin delivery system based on competitive binding of glucose and glycosylated insulin. In: Kost J (ed) Pulsed and self-regulated drug delivery. CRC, Boca Raton, pp 187–199

    Google Scholar 

  12. Jo S-M, Kim J-C (2009) Glucose-triggered release from liposomes incorporating poly(N -isopropylacrylamide-co-methacrylic acid-co-octadecylacrylate) and glucose oxidase. Colloid Polym Sci 287:379–384

    Article  CAS  Google Scholar 

  13. Drummond DC, ZM LJC (2000) Current status of pH-sensitive liposomes in drug delivery. Prog Lipid Res 39:409–460

    Article  CAS  Google Scholar 

  14. Jo S-M, Lee HY, Kim J-C (2008) Glucose-sensitive liposomes incorporating hydrophobically modified glucose oxidase. Lipids 43:937–943

    Article  CAS  Google Scholar 

  15. Choi J-H, Lee HY, Kim J-C (2008) pH-Sensitivity and air/water interfacial activity of poly(N-isopropylacrylamide-co-methacrylic acid-co-octadecyl acrylate). J Appl Polym Sci 108:3707–3712

    Article  CAS  Google Scholar 

  16. Choi J-H, Lee HY, Kim J-C (2008) Release behavior of freeze-dried alginate beads containing poly(N-isopropylacrylamide) copolymers. J Appl Polym Sci 110:117–123

    Article  CAS  Google Scholar 

  17. Cho SM, Lee HY, Kim J-C (2008) Liposomes incorporating hydrophobically modified glucose oxidase. Korean J Chem Eng 25:390

    Article  CAS  Google Scholar 

  18. Bergmeyer HU, Gawehn K, Grassl M (1974) Methods of enzymatic analysis. Academic, New York

    Google Scholar 

  19. Jeong B, Gutowska A (2002) Lessons from nature: stimuli-responsive polymers and their biomedical applications. Trends Biotechnol 20:305–311

    Article  CAS  Google Scholar 

  20. Schaeffer HE, Krohn DL (1982) Liposomes in topical drug delivery. Invest Ophth Vis Sci 22:220–227

    CAS  Google Scholar 

  21. Gregoriadis G (1984) Liposome technology, Vols. I, II, III. CRC, Boca Raton

    Google Scholar 

  22. New RRC (1990) Introduction. In: New RRC (ed) Liposomes: A practical approach. IRL, New York, pp 1–22

    Google Scholar 

  23. Simoes S, Moreira JN, Fonseca C, Duzgunes N, Pedroso de lima MC (2004) On the formulation of pH-sensitive liposomes with long circulation times. Adv Drug Deliv Rev 56:947–965

    Article  CAS  Google Scholar 

  24. Coorssen JR, Rand RP (1995) Structural effects of neutral lipids on divalent cation-induced interactions of phosphatidylserine-containing bilayers. Biophys J 68:1009–1018

    Article  CAS  Google Scholar 

  25. Slepushkin VA, Simões S, Dazin P, Newman MS, Guo LS, Pedroso Lima MC, Düzgünes N (1997) Sterically stabilized pH-sensitive liposomes: intracellular delivery of aqueous contents and prolonged circulation in vivo. J Biol Chem 272:2382–2388

    Article  CAS  Google Scholar 

  26. Litzinger DC, Huang L (1992) Phosphatidylethanolamine liposomes: drug delivery, gene transfer and immunodiagnostic applications. Biochim Biophys Acta 1113:201–227

    CAS  Google Scholar 

  27. Ellens H, Bentz J, Szoka FC (1984) pH-Induced destabilization of phosphatidyl ethanolamine-containing liposomes: role of bilayer contact. Biochemistry 23:1532–1538

    Article  CAS  Google Scholar 

  28. Cullis PR, Kruijff BD (1979) Lipid polymorphism and the functional roles of lipids in biological membranes. Biochim Biophys Acta 559:399–420

    CAS  Google Scholar 

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Acknowledgments

This work was supported by a grant (S120708L1701104) from Special Forest Research Program, Korea Forestry Research Program, Korea Forest Service, Republic of Korea.

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  1. School of Biotechnology & Bioengineering and Institute of Bioscience and Biotechnology, Kangwon National University, 192-1, Hyoja 2-dong, Chunchon, Kangwon-do, 200-701, Korea

    Yeon Ji Hong, Hyeon Yong Lee & Jin-Chul Kim

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  1. Yeon Ji Hong
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Correspondence to Jin-Chul Kim.

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Hong, Y.J., Lee, H.Y. & Kim, JC. Alginate beads containing pH-sensitive liposomes and glucose oxidase: glucose-sensitive release. Colloid Polym Sci 287, 1207–1214 (2009). https://doi.org/10.1007/s00396-009-2084-2

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  • DOI: https://doi.org/10.1007/s00396-009-2084-2

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