Abstract
Solubilization of different zwitterionic phospholipid vesicles structures such as L-α-phosphatidylcholine (PC) and 1,2-didecanoyl-sn-glycero-3-phosphocholine (DPC) have been studied in aqueous bulk by using zwitterionic surfactant dimethylhexadecylammoniopropanesulfonate (HPS). This has been done by studying the aggregation of HPS in pure water and in the presence of 7–36 μM of fixed concentrations of each lipid with the help of pyrene fluorescence intensity (I 1/I 3) measurements. The fluorescence measurements showed that HPS monomers undergo two kinds of aggregation process, which were identified by the three breaks in a plot of pyrene fluorescence versus HPS concentration. The first two breaks, C 1 and C 2, indicate the onset and completion of vesicle solubilization respectively, upon incorporation of HPS monomers into the vesicles and led to solubilization in the form of mixed micelles. This process was not clearly visible at low lipid concentration. We evaluated the partition coefficient (K), which defines the degree of partitioning of surfactant monomers into the vesicles with respect to the aqueous medium. A high K value of HPS-lipid aggregates indicates the stronger interactions between surfactant and lipid vesicles. The K values evaluated for PC and DPC are quite close to each other, which indicates that K values were independent of phospholipid chain length.
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References
Helinius A, Simons K (1975) Biochim Biophys Acta 415:29
Lichteunberg D, Robson EA, Dennis EA (1983) Biochem Biophys Acta; Rev Biomemb 285:738
Elworthy PH, Florence AT, Macfarlane CB (1968) In: Solubilization of surface active agent. Chapman and Hall, London
Le Maire M, Champeil P, Moller JV (2000) Biochim Biophys Acta 1508:86
Lichtenberg D (1985) Biochim Biophys Acta 821:470
Jones MN (1999) Int J Pharmaceutic 177:137
Sehgal P, Doe H, Bakshi MS (2002) J Colloid Interface Sci 252:195
Johnsson M, Edwards K (2000) Langmuir 16:8632
Cocera M, Lopez O, Estelrich J, Parra JL, De la Maza A (2000) Langmuir 16:4068
Sehgal, P, Doe H, Doe, Bakshi MS (2003) J Surfactants Detergents 6:31
Helinius, A, McCaslinDR, Fries E, Tanford C (1979) Methods Enzymol 56:734
Tanford C, Reynolds JA (1979) Biochim Biophys Acta 457:133
Ribera R, Velazquez (1999) Langmuir 15:6686
Brito RM, Vaz WLC (1986) Anal Biochem 152:250
Shinoda, K, Arai H, Murata (1971) J Colloid Interface Sci 37:2223
Kalyanasundaram K, Thomas JK (1977) J Am Chem Soc 99:2039
Inoue T (1996) Interaction of surfactant with phospholipids. In: M Rosoff (ed) Vesicles, Surf Sci Ser, V 62. Dekker Inc, New York pp 151–195
Dennis EA (1986) Adv Colloid Interface Sci 26:155
Inoue T, Miyakawa K, Shimozawa R (1986) Chem Phys Lipids 42:261
Inoue T, Muraoka Y, Fukushima K, Shimozawa R (1988) Chem Phys Lipids 48:189
Ueno M (1989) Biochemistry 28:5631
Schurtenberger P, Mazer N, King KW (1985) J Phys Chem 89:1042
Green RJ, Su TJ, Joy H, Lu JR, (2000) Langmuir 16:5797
De la Maza A, Parra JL (1995) J Controlled Release 37:33
Acknowledgements
P.S. is thankful to the Monbusho and CNRS-IBCP for financial support as a doctoral and postdoctoral fellow, respectively. D.O. acknowledges support from the Danish Technical Science Research Council.
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Sehgal, P., Doe, H., Sharma, M. et al. Interaction between phospholipid vesicular structures with long chain zwitterionic surfactants. Colloid Polym Sci 282, 677–683 (2004). https://doi.org/10.1007/s00396-003-0992-0
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DOI: https://doi.org/10.1007/s00396-003-0992-0