Several epidemiological studies have analyzed the associations between red and processed meat and bladder cancer risk but the shape and strength of the associations are still unclear. Therefore, we conducted a dose–response meta-analysis to quantify the potential association between red and processed meat and bladder cancer risk.
Relevant studies were identified by searching the PubMed database through January 2016 and reviewing the reference lists of the retrieved articles. Results were combined using random-effects models.
Five cohort studies with 3262 cases and 1,038,787 participants and 8 cases–control studies with 7009 cases and 27,240 participants met the inclusion criteria. Red meat was linearly associated with bladder cancer risk in case–control studies, with a pooled RR of 1.51 (95% confidence interval (CI) 1.13, 2.02) for every 100 g increase per day, while no association was observed among cohort studies (P heterogeneity across study design = 0.02). Based on both case–control and cohort studies, the pooled relative risk (RR) for every 50 g increase of processed meat per day was 1.20 (95% CI 1.06, 1.37) (P heterogeneity across study design = 0.22).
This meta-analysis suggests that processed meat may be positively associated with bladder cancer risk. A positive association between red meat and risk of bladder cancer was observed only in case–control studies, while no association was observe in prospective studies.
Bladder cancer is the fifth most common cancer among men and the fourteenth among women with an estimated number of 429,000 cases worldwide in 2012 . Bladder cancer is rather common in developed countries (North America and Europe), and it is more frequent among persons aged 75 or older . Mortality rates have been stable over the last decade with 165,000 estimated deaths in 2012 . A few risk factors have recently been linked to the etiology of bladder cancer. Apart from age and gender, cigarette smoking and specific occupational exposures are considered the most important risk factors [3, 4]. Identification of additional modifiable risk factors such as diet may enhance primary prevention.
Recently two meta-analyses summarized the body of evidence concerning red and processed meat consumption and risk of bladder cancer [5, 6]. Results from the review by Wang et al.  indicated an increased risk of bladder cancer of 17 and 10% for high red meat and high processed meat consumption, respectively. The more recent review by Li et al. , on the other hand, found a significant association for processed meat, with a 22% increased risk of bladder cancer for high consumption but not for red meat consumption. Both meta-analyses, however, were based only on contrasting risk estimates for the highest vs. the lowest category of meat consumption, and this has some limitations when the exposure distribution vary substantially across studies. In the review by Li et al. , one of the included studies  conducted in Uruguay, for instance, used 0–150 g/day of red meat consumption (median of 85 g/day) as the lowest category. In another study conducted in the USA , >58.5 g/day was the highest category for red meat consumption.
Our aim is to describe variation in bladder cancer risk across the whole range of the exposure distribution. A dose–response analysis is more efficient and less sensitive to heterogeneity of the exposure across different study populations. Therefore, we conducted a dose–response meta-analysis to clarify and quantify the potential association between red and processed meat and bladder cancer risk.
Materials and methods
Literature search and selection
Eligible studies were identified by searching the PubMed database through July 2016, with the terms [“bladder” AND (“carcinoma” or “cancer” or “tumor” OR “neoplasms”)] AND (“meat” or “beef” or “pork” or “lamb”). In addition, the reference lists of the retrieved articles were examined to identify additional reports. The search was restricted to studies written in English and carried out in human. We performed this meta-analysis accordingly to the Meta-Analysis of Observational Studies in Epidemiology (MOOSE) guidelines . Two authors (A.C. and A.D.) independently retrieved the data from studies on the association between red and processed meat and risk of bladder cancer. Discrepancies were discussed and resolved.
Studies were eligible if they met the following criteria: (1) the study was a cohort or case–control study; (2) the exposure of interest was red meat and/or processed meat; (3) the outcome was incidence of bladder cancer; (4) the authors reported measures of association (hazard ratio, relative risk, odds ratio) with the corresponding confidence intervals for three or more categories for red or processed meat consumption. In case of multiple reports on the same study population, we included only the most comprehensive or recent one.
From each study, we extracted the following information: first author’s surname, year of publication, study design, country where the study was conducted, study period, exposure definition, unit of measurement, number of cases, study size, confounding variables, and measure of associations for all the categories of meat consumption together with their confidence intervals. Given the low prevalence of bladder cancer, the odds ratios were assumed approximately the same as the relative risks (RRs). When several risk estimates were available, we included those reflecting the greatest degree of adjustment.
We used the method described by Greenland and Longnecker  and Orsini et al.  to reconstruct study-specific trend from aggregated data, taking into accounts the covariance among the log RR estimates. Risk estimates from studies not reporting information about the number of deaths and study size did not allow reconstruction of the covariance and were assumed independent. Potential nonlinear associations were assessed by use of restricted cubic splines with three knots located at the 10th, 50th, and 90th percentiles of the exposure distribution. An overall P value was calculated by testing that the regression coefficients were simultaneously equal to zero. A P value for nonlinearity was obtained by testing that the coefficient of the second spline term was equal to zero .
Since studies used different units to express meat consumption (e.g., servings/day, grams/day, grams per 1000 kcal/day), we converted frequency of consumption using 120 and 50 g as the average portion sizes for red and processed meat, respectively. We chose those values in accordance with previous meta-analyses on meat consumption and other types of cancer [13, 14] and results from both the Continuing Survey of Food Intakes by Individuals  and the European Prospective Investigation into Cancer and Nutrition . Meat consumption reported in grams per 1000 kcal/day was converted to g/day using the average energy intake reported in the original articles. Within each exposure category, the median or mean value was assigned to the corresponding RRs. If not reported, we assigned the midpoint of the upper and lower boundaries as average consumption. If the upper bound of the highest category was not reported, we assumed that the category had the same width as the contiguous one. When RRs were reported only for single food items (e.g., separately for beef and pork), we combined them using a fixed-effects model and used the pool estimate as summary measure.
A random-effects meta-analysis was adopted to acknowledge heterogeneity across study findings. Statistical heterogeneity was further assessed by using the Q test (defined as a P value less than 0.10) and quantified by R b statistic . Meta-regression models were employed to explain residual heterogeneity. Differences in dose–response curves between subgroups of studies were tested as described by Berlin et al. . Evaluation of goodness-of-fit for the final models was assessed using the set of tools presented by Discacciati et al. . Publication bias was investigated using the Egger asymmetry test .
We performed sensitivity analyses (1) excluding studies where red meat definition included also some items of processed meat; (2) excluding studies that did not adjust for energy intake; (3) evaluating alternative average portion sizes for red meat (100 and 140 g) and processed meat (30 and 70 g) consumption. All statistical analyses were conducted with the dosresmeta  and metafor  packages in R (R Foundation for Statistical Computing, Vienna, Austria) . P values less than 0.05 were considered statistically significant.
The search strategy identified 146 articles, 108 of which were excluded after review of the title or abstract (Fig. 1). Of the 38 eligible papers 14 were excluded because they did not meet the inclusion criteria (not original articles, outcome different from bladder cancer, or not reporting risk estimates with their confidence intervals). The reference lists of the remaining 24 articles were checked for additional pertinent reports, and 5 additional papers were identified. We further excluded 16 additional articles: 8 presented duplicated publications [24,25,26,27,28,29,30,31]; 3 analyzed bladder and other urinary cancer together [32,33,34]; 3 did not report enough data for a dose–response analysis [35,36,37]; and 2 did not report results for red or processed meat consumption [16, 38]. Thus, the meta-analysis included 13 independent epidemiological studies [7, 8, 31, 39,40,41,42,43,44,45,46,47,48,49].
The main characteristics of the 13 epidemiological studies included in the meta-analysis are presented in Table 1. Five cohort studies [39,40,41,42,43] with 3262 cases and 1038,787 participants and 8 cases–control studies, of which 4 population-based [8, 44, 46, 47] and 4 hospital-based [7, 45, 48, 49], with 7009 cases and 27,240 participants evaluated the relation between red and/or processed meat and risk of bladder cancer. Two articles [39, 49] reported results only for red meat, while one  only for processed meat. Definition of meat and red meat varied across studies but generally included beef, veal, pork, and lamb for red meat, and bacon, ham, salami, sausages, and hot dogs for processed meat. Two cohort studies [39, 40] included also processed meat in the definition of red meat, and one study  included only results for specific food items. One study  reported results only for liver intake and was not included in the analysis of red meat. Another study  analyzed preserved meat consumption and, given the limited range of exposure (up to 1/week), was excluded from the analysis of processed meat.
Only 3 studies [40, 46, 48] considered different cooking methods and doneness levels for meat consumption. None of them found evidence of an association between preparation methods and bladder cancer. Different units were used to report meat consumption: servings/week (7 studies), grams per 1000 kcal per day (3 studies), and grams per day (3 studies). Five studies were conducted in the USA, 4 in Europe, and 1 each in Canada, Uruguay, China, and Japan. All the studies were carried out in both men and women, and only one study  reported results separately by gender. All the studies provided measure of associations adjusted for age, gender, and smoking. Four studies did not adjust for energy intake [43,44,45, 49]. Other common adjusting variables were other food groups (8 studies), BMI (6 studies), education (6 studies). Additional covariates were less consistent across studies.
Association between red meat consumption and risk of bladder cancer
We found a statistically significant association between red meat consumption and risk of bladder cancer (P = 0.009; P nonlinearity = 0.74) (Online Resource 1). The summary RR for an increment of 100 g per day of red meat was 1.22 (95% CI 1.05, 1.41). There was substantial between-studies heterogeneity (R b = 67%, P < 0.01). Egger’s regression test did not suggest the presence of substantial publication bias (P = 0.14).
There was statistical heterogeneity according to study design (P for heterogeneity = 0.02). The pooled RR restricted to the cohort studies was 1.01 (95% CI 0.97, 1.06) for an increment of 100 g per day of red meat with no significant heterogeneity (R b = 0%, P = 0.62) (Figure 2). The deviance test did not detect lack of fit (D = 24, df = 18, P = 0.17). In case–control studies, the corresponding pooled RR was 1.51 (95% CI 1.13, 2.02) with substantial heterogeneity among studies (R b = 81%, P < 0.01) and overall indication of poor fit (D = 44, df = 18, P < 0.01).
No differences were found according to study location (P for heterogeneity = 0.7), units of measurement (P for heterogeneity = 0.38), and selection of controls (P for heterogeneity = 0.65). Excluding those studies with also processed meat in the definition of red meat, the pooled RRs were 1.27 (95% CI 1.03, 1.57) overall and 0.95 (95% CI 0.82, 1.11) restricted to cohort studies. The pooled RR for an increment of 100 g of red meat per day was 1.14 (95% CI 0.99, 1.31) based on studies that adjusted for energy intake. In the sensitivity analysis for alternative average portion sizes of red meat, the results did not substantially change. The pooled RR for an increment of 100 g of red meat per day was 1.27 and 1.19 for assigned portions of 140 g per day and 100 g per day, respectively.
For an increment of four servings per week of red meat (120 g per servings), the summary RR of bladder cancer was 1.15 (95% CI 1.03, 1.27) overall, 1.01 (95% CI 0.98, 1.04) for cohort studies, and 1.32 (95% CI 1.08, 1.62) for case–control studies.
Association between processed meat consumption and risk of bladder cancer
We found a statistically significant association between processed meat intake and bladder cancer with no departure from linearity (P = 0.005, P nonlinearity = 0.92) (Online Resource 2). Every 50 g increase in processed meat per week was associated with a 20% (95% CI 6, 37) increase in risk of bladder cancer with moderate heterogeneity (R b = 38%, P = 0.07). Egger’s regression test did not detect publication bias (P = 0.21). No evidence of lack of fit was observed (D = 43, df = 34, P = 0.14). The test did not detect significant differences between case–control and cohort studies (P for heterogeneity = 0.22). Stratified analysis provided a RR of 1.10 (95% CI 0.95, 1.27) and 1.31 (95% CI 1.06, 1.63) for cohort and case–control studies, respectively (Fig. 3).
The associations were similar across strata of study location (P for heterogeneity = 0.68), units of measurement (P for heterogeneity = 0.71), and selection of controls (P for heterogeneity = 0.46). Exclusion of studies that did not adjust for energy intake provided a pooled RR of 1.24 (95% CI 1.07, 1.43). Similar results were observed for alternative average portion sizes of 30 g per day and 70 g per day with pooled RR, respectively, of 1.14 and 1.36 for an increment of 50 g per day of processed meat.
For an increment of four servings per week of processed meat (50 g per servings), the summary RR of bladder cancer was 1.11 (95% CI 1.03, 1.20) overall, 1.06 (95% CI 0.97, 1.15) for cohort studies, and 1.17 (95% CI 1.03, 1.32) for case–control studies.
Findings from this dose–response meta-analysis of five cohort and eight case–control studies suggest that processed meat consumption is positively associated with risk of bladder cancer. An increment of 50 g of processed meat per day was associated with 20% increased risk of bladder cancer. Red meat consumption was associated with bladder cancer only in case–control studies, with a 51% increased risk of an increment of 100 g per day, while no association was observed among the prospective studies.
Meat, in particular processed meat, is a potential risk factor for several cancers, with the most convincing evidence for colorectal cancer . In 2015, the International Agency for Research on Cancer classified processed meats as carcinogenic to humans (Group 1) and red meat as probably carcinogenic to humans . The contribution of meat to the etiology of bladder cancer may be explained by different mechanisms, given that many nutrients are excreted through the urinary tract . The most established mechanism involves the formation of endogenous nitrosamines from nitrites that are particularly abundant in processed meats . Experimental studies have shown that some nitrosamine metabolites induce bladder tumors in rodents [54, 55]. Further support for at potential role of nitrosamines in bladder carcinogenesis is that cigarette smoking is a strong risk factor for bladder cancer and tobacco smoke is a main source of exogenous exposure to nitrosamines. Consumption of red meat could potentially increase the risk of bladder cancer through heterocyclic amines and polycyclic aromatic hydrocarbons, which can be generated from high temperature cooking . Heterocyclic amines and polycyclic aromatic hydrocarbons have been consistently shown to be carcinogenic in animal studies [56, 57].
A direct comparison with the results of previous reviews [5, 6] is difficult since they were based on study-specific risk estimates for high versus low categories of meat consumption, which varied substantially across studies. The directions of the associations, however, were consistent, even though an association was found only for processed meat in the meta-analysis by Lin et al. . As in the review by Wang et al. , case–control studies provided stronger risk estimates as compared to prospective studies.
Among several potential explanations, case–control studies generally assess the exposure after diagnosis, and therefore, recall bias may lead to differential misclassification between cases and controls. Considering the limited knowledge of the role of meat consumption on the development of bladder cancer , such classification errors are likely to be similar among cases and controls. On the other hand, half of the control studies used hospital-based controls which may inflate the pooled association in case controls have been recruited for conditions linked with changes in meat consumption. Although based on limited number of studies, we did not observed differences in results between hospital-based and population-based case–control studies. Different participation rates related to exposure or severity of diseases may also be a selection bias among case–control studies. In addition, the time between diagnosis and the exposure assessment is generally shorter for case–control studies; hence, it may not reflect long-term exposure because of changes in dietary patterns. On the other hand, in cohort studies participants may alter their dietary intake during the follow-up, which may bias results toward the null hypothesis of no association. One of the included cohort studies  analyzed repeated dietary measurements over time and observed stronger associations when using cumulative update date and when removing participant who had change their meat consumption.
Strength of this review is the dose–response analysis, which better takes into account the quantitative nature and heterogeneity of the exposure. In our analysis, all the information about meat consumption, including intermediate categories, contributed to the pooled associations. Another strength is the large number of cases that provided high statistical power to detect associations of moderate magnitude. Lastly, no evidence of publication bias was observed.
This meta-analysis also had potential limitations. Pooling results from epidemiological studies do not solve the problem of residual confounding, which inherently affects individual studies. All of the included studies, however, adjusted for main known risk factors for bladder cancer such as age, gender, and smoking, and some studies also adjusted for energy intake, BMI, education, and other food groups. Excluding those studies not adjusting for energy intake did not change the overall results, suggesting that energy intake may have a limited impact on developing bladder cancer. Second, red and processed meat definition varied across study and this may partially contribute to the observed heterogeneity. Different units of measurements were also used to report risk estimates for meat consumption, and we had to assume average portion sizes when meat consumption was reported as servings. Nevertheless, stratified analysis for different types of measurements and sensitivity analysis for alternative portion sizes did not find substantial differences in results. Third, it was not possible to investigate the association between different meat-cooking methods and bladder cancer because only three articles reported such information. However, none of them found an increase in bladder cancer risk with any of the cooking methods. Fourth, statistical heterogeneity was observed in our analysis as in the previous two reviews [5, 6] but was mainly explained by different study design. After stratification, moderate heterogeneity was still observed among case–control studies, while cohort studies provided more homogenous results.
In conclusion, results from this dose–response meta-analysis suggest that processed meat consumption may be positively associated with risk of bladder cancer. Positive association between red meat and risk of bladder cancer was observed only in case–control studies, while no association was observed in prospective studies.
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This work was partly supported by Young Scholar Award from the Karolinska Institutet’s Strategic Program in Epidemiology.
All authors (AC, SL, AD, AW, and NO) participated both in the study design and in writing the manuscript. AC and AD participated in the data collection. AC analyzed the data and wrote the manuscript under the supervision of NO. SL and AW interpreted the results and critically reviewed the paper. All authors read and approved the final manuscript.
Conflict of interest
Authors declare that they have no conflict of interest.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Dose–response relation between red meat consumption and risk of bladder cancer, assuming a linear-response model in random-effects meta-analysis. The dotted line represents the predicted curve arising from a restricted cubic spline model. The solid line represents the linear trend and the dashed lines its confidence limits. The median value of the lowest reference category (15 g/day) was used as referent. The relative risks are plotted on the log scale.
Dose–response relation between processed meat consumption and risk of bladder cancer, assuming a linear-response model in random-effects meta-analysis. The dotted line represents the predicted curve arising from a restricted cubic spline model. The solid line represents the linear trend and the dashed lines its confidence limits. The median value of the lowest reference category (5 g/day) was used as referent. The relative risks are plotted on the log scale.
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Crippa, A., Larsson, S.C., Discacciati, A. et al. Red and processed meat consumption and risk of bladder cancer: a dose–response meta-analysis of epidemiological studies. Eur J Nutr 57, 689–701 (2018). https://doi.org/10.1007/s00394-016-1356-0
- Red meat
- Processed meat
- Bladder cancer