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Vitamin B2, vitamin B12 and total homocysteine status in children and their associations with dietary intake of B-vitamins from different food groups: the Healthy Growth Study

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Abstract

Purpose

To examine the associations between the dietary intakes of certain B-vitamins from different food sources with the relevant plasma status indices in children.

Methods

A representative subsample of 600 children aged 9–13 years from the Healthy Growth Study was selected. Dietary intakes of vitamins B2, B12, B6 and folate derived from different food sources were estimated. Plasma levels of vitamin B2 (or riboflavin), methylmalonic acid (MMA) and total homocysteine (tHcy) were also measured.

Results

Plasma concentrations of vitamin B2 below 3 μg/L were found in 22.8 % of the children. Children in the lower quartile of dietary vitamin B2 intake were found to have the lowest plasma vitamin B2 levels compared to children in the upper three quartiles (5.06 ± 7.63 vs. 6.48 ± 7.88, 6.34 ± 7.63 and 6.05 ± 4.94 μg/L respectively; P = 0.003). Regarding vitamin B12 children in the lower quartile of dietary intake had higher mean plasma tHcy levels compared to children in the upper two quartiles, respectively (6.00 ± 1.79 vs. 5.41 ± 1.43 and 5.46 ± 1.64 μmol/L; P = 0.012). Positive linear associations were observed between plasma vitamin B2 levels and dietary vitamin B2 derived from milk and fruits (β = 0.133; P = 0.001 and β = 0.086; P = 0.037). Additionally, nonlinear associations were also observed between plasma vitamin B2 levels and vitamin B2 derived from red meat, as well as between tHcy levels and vitamins B12 and B6 derived from milk; vitamins B12, B6 and folate derived from cereal products and folate derived from fruits.

Conclusion

A considerably high prevalence of poor plasma vitamin B2 status was observed in children. The intake of milk, fruits and cereals was associated with more favorable tHcy levels, while the intake of milk and fruits with more favorable plasma B2 levels. However, these findings need to be further confirmed from controlled dietary intervention studies examining the modulation of biomarkers of B-vitamins.

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Acknowledgments

The biochemical analyses of vitamin B2, MMA and tHcy levels were supported by a research grant from FrieslandCampina. The authors are indebted to the research team members of the Healthy Growth Study, as well as to the parents and children for their willingness to participate in the study.

Funding

The biochemical analyses of vitamin B2, MMA and tHcy levels were supported by a research grant from FrieslandCampina. FrieslandCampina was not involved in the recruitment of participants, data collection, management and analysis, as well as in the final set of results of the current study.

Author contributions

YM and EvdH contributed to the study design. YM, GM, CM and EG were responsible for data collection, management and statistical analyses. All authors contributed to the writing and revising of the manuscript.

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Correspondence to Yannis Manios.

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Conflict of interest

EvdH and RD are employees at FrieslandCampina. None of the other authors have any potential conflict of interest. Any opinions, findings, conclusions or recommendations expressed in the current study are those of the authors and do not necessarily reflect the views of FrieslandCampina.

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Manios, Y., Moschonis, G., Dekkers, R. et al. Vitamin B2, vitamin B12 and total homocysteine status in children and their associations with dietary intake of B-vitamins from different food groups: the Healthy Growth Study. Eur J Nutr 56, 321–331 (2017). https://doi.org/10.1007/s00394-015-1082-z

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