Abstract
Leukocyte emigration from blood to sites of inflammation involves sequential interaction of specific adhesion molecules expressed by both leukocytes and endothelial cells. The central steps in leukocyte-endothelial adhesive interactions are leukocyte rolling, sticking, and transmigration. This study investigated the effect of monoclonal antibodies against CD54 (ICAM-1) and CD11b (αM-chain of MAC-1) on intestinal inflammation. Anti-CD54 and anti-CD11b were tested in rats with indomethacin-induced chronic ileitis. Macroscopic changes were assessed by a modified version of the Wallace et al. score. Leukocyte rolling and sticking were investigated by intravital microscopy. Results show that indomethacin administration led to a chronic inflammatory response characterized by significant increase (P<0.05) in rolling (from 5.41±2.87 to 32.41±15.03 100 µm–1 s–1) and sticking (from 0.16±0.18 to 9.11±5.3 100 µm–1 s–1) leukocytes. After antibody treatment only the anti-CD11b group showed significant (P<0.05) reduction in rolling (from 32.41±15.03 to 6.6±2.7 100 µm–1 s–1) and sticking (from 9.11±5.3 to 0.07±0.09 100 µm–1 s–1) leukocytes. This was also the case for macroscopic changes. Indomethacin led to a rise in the Wallace score from 0 to 4.29±0.76 points (P<0.05) and anti-CD11b to a reduction from 4.29±0.76 to 1.29±1.11 points (P<0.05). Anti-CD54 and combined anti-CD11b/CD54 administration was not followed by significant changes. Therefore we suggest that leukocyte-based CD11b but not endothelial-based CD54 contributes most to leukocyte adhesion in the setting of indomethacin-induced ileitis in rats.
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Accepted: 7 September 1999
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Krieglstein, C., Anthoni, C., Laukötter, M. et al. Effect of anti-CD11b (αM-MAC-1) and anti-CD54 (ICAM-1) monoclonal antibodies on indomethacin induced chronic ileitis in rats. Int J Colorect Dis 14, 219–223 (1999). https://doi.org/10.1007/s003840050214
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DOI: https://doi.org/10.1007/s003840050214