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Electrogenic transport, oxygen consumption, and sensitivity to acute hypoxia of human colonic epithelium

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Abstract

Introduction

It is recognized that epithelial ion transport depends on oxygen supply, but this dependence has not been characterized in the human colon in vitro despite its surgical and clinical implications.

Purposes

The aim of this study is to measure the oxygen consumption of colonic epithelium under conditions which preserve vectorial ion transport and to assess the sensitivity of the human colonic epithelium short-circuit current (I sc) to acute hypoxia induced in vitro.

Methods

Isolated mucosa preparations from human sigmoid colon were placed in a modified Ussing chamber which allows simultaneous measurement of short-circuit current (I sc) and oxygen consumption (QO2). In separate experiments, the sensitivity to acute hypoxia induced in a conventional Ussing chamber was assessed.

Results

Basal mean ± SEM values (n = 8) were I sc = 3.3 ± 0.5 μEq h−1 cm−2 and QO2 = 8.09 ± 0.55 μmol h−1 cm−2. The contribution of the serosal side to the oxygen supply was higher than that of the mucosal side (p = 0.0023). Ouabain reduced I sc by 70% (P < 0.0001) and QO2 by 26% (n = 8; P = 0.0009), suggesting that a large fraction of QO2 is needed to support ouabain-sensitive electrogenic transport. Induction of hypoxia at both sides of the Ussing chamber caused a rapid decrease in I sc after 2 min. I sc was also significantly depressed when hypoxia was induced by 5 min in the serosal side (n = 6, P < 0.0001), but was unaffected by hypoxia induced in the mucosal side.

Conclusion

The present results allow a better understanding of the clinical consequences of acute hypoxia on intestinal ion transport.

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Abbreviations

I sc :

Short-circuit current

PD:

Transepithelial potential difference

QO2 :

Epithelial oxygen consumption

R te :

Transepithelial resistivity

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Acknowledgments

The authors thank the collaboration and advice of Dr. Rolando Valent, Dr. Héctor Viotti, and Dr. Dino Sánchez De Simone from the Hospital Español and of Prof. Daniel Matus and Dr. Francisco Bettalemi from the Hospital Militar of Mendoza. This work was funded by a grant from the National University of Cuyo (Secretaría de Ciencia, Técnica y Posgrado).

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Authors and Affiliations

  1. Area of Biological Physics, Department of Morphology and Physiology, Faculty of Medical Sciences, National University of Cuyo, Mendoza, MZ, 5500, Argentina

    Graciela E. Carra, Jorge E. Ibáñez & Fernando D. Saraví

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  1. Graciela E. Carra
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  2. Jorge E. Ibáñez
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  3. Fernando D. Saraví
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Correspondence to Fernando D. Saraví.

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Carra, G.E., Ibáñez, J.E. & Saraví, F.D. Electrogenic transport, oxygen consumption, and sensitivity to acute hypoxia of human colonic epithelium. Int J Colorectal Dis 26, 1205–1210 (2011). https://doi.org/10.1007/s00384-011-1215-7

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