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Phospholipids reduce the intraperitoneal adhesion of colonic tumor cells in rats and adhesion on extracellular matrix in vitro

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Objective

To evaluate the influence of intraperitoneal treatment with phospholipids on the formation of peritoneal carcinosis after inoculation of colonic tumor cells in rats.

Summary background data

The presence of tumor cells in the peritoneal cavity serves as a prognostic marker for postoperative survival after resection of gastrointestinal cancer. Intraperitoneal tumor cell attachment is a pivotal step in developing peritoneal carcinosis. Intraabdominal application of phospholipids resulted in a significant decrease of adhesion formation, especially at sites of peritoneal lesions.

Methods

2×106 colonic tumor cells (DHD/K12/TRb) were injected intraperitonely in female BD-IX rats. A total of 90 rats were divided into three groups with treatments of phospholipids at 75 mg/kg or 150 mg/kg bodyweight or sodium chloride at 0.9% in the control group. The treatment groups were subdivided into animals with defined peritoneal lesions and animals without lesions. After 30 days, the extent of peritoneal carcinosis was determined by measuring the tumor volume, the area of tumor attachment and the Peritoneal Cancer Index. Over a 90-day observation period, the survival rate was analyzed. In vitro, we examined the reduction of tumor cell adhesion on extracellular matrix components after treatment with phospholipids. Microtiter plates were coated with laminin, fibronectin or collagen IV for adhesion experiments.

Results

In our study, we found a significant reduction of peritoneal dissemination with respect to all evaluation methods after treatment with phospholipids at 150 mg/kg in animals without peritoneal lesions. This could not be achieved using the lower concentration of phospholipids (75 mg/kg). In vitro, the maximum reductions of tumor cell adhesion by phospholipids compared with the control values for laminin and fibronectin were 46% and 37%, respectively, whereas for collagen IV the reduction was only 24% (p<0.0001).

Conclusions

A new method of prevention of intraperitoneal tumor cell adhesion, possibly leading to a reduced incidence of peritoneal carcinosis after surgery of gastrointestinal tumors, is introduced.

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Acknowledgments

This work was supported by Fresenius Kabi, Bad Homburg, Germany. Permission of local authorities: file number AZ50.203.2-AC 18, 44/00.

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Authors and Affiliations

  1. Department of Surgery, University Clinic Aachen, Pauwelsstrasse 30, 52057, Aachen, Germany

    Marc Jansen, Karl-Heinz Treutner, Jens Otto, Britta Schmitz, Stefan Mueller & Volker Schumpelick

  2. Interdisciplinary Center of Clinical Research (IZKF) BIOMAT, University Clinic Aachen, Pauwelsstrasse 30, 52057, Aachen, Germany

    Petra Lynen Jansen

  3. Institute of Biometry, University Clinic Aachen, Pauwelsstrasse 30, 52057, Aachen, Germany

    Claudia Weiss

  4. Institute of Pathology, University Clinic Aachen, Pauwelsstrasse 30, 52057, Aachen, Germany

    Lothar Tietze

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  1. Marc Jansen
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Correspondence to Marc Jansen.

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Jansen, M., Treutner, KH., Lynen Jansen, P. et al. Phospholipids reduce the intraperitoneal adhesion of colonic tumor cells in rats and adhesion on extracellular matrix in vitro. Int J Colorectal Dis 19, 525–532 (2004). https://doi.org/10.1007/s00384-004-0611-7

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  • DOI: https://doi.org/10.1007/s00384-004-0611-7

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