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Beneficial effects of Etanercept on experimental necrotizing enterocolitis

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Abstract

Purpose

Tissue damage in necrotizing enterocolitis (NEC) of infants occurs as a result of an uncontrolled inflammatory response. The aim of this study was to investigate any potential anti-inflammatory effects that Etanercept may have on the inflammatory response in an experimental NEC model in newborn rats.

Methods

Newborn pups were randomized into three groups immediately after birth (Control, NEC + Placebo and NEC + Etanercept). Pups in the NEC + Placebo and NEC + Etanercept groups were subjected to an NEC-inducing protocol (hypercarbia, hypothermia and hyperoxia) twice a day for 3 days. Pups in the NEC + Etanercept group were given an intraperitoneal injection of Etanercept. Rats were harvested for biochemical and histopathological examinations.

Results

The histopathological injury score of rats in the NEC + Placebo group was significantly higher compared to the NEC + Etanercept and Control groups (p < 0.05 for both comparisons). Tissue levels of tumor necrosis factor-α, interleukin-1β, and malondialdehyde were higher in the placebo group compared to the Etanercept group.

Conclusion

Our results suggest that Etanercept attenuates intestinal tissue damage in NEC by reducing inflammation and blocking the production of free-oxygen radicals, while also reducing tissue levels of tumor necrosis factor-α and interleukin-1β.

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Acknowledgments

No financial assistance was received to support this study.

Conflict of interest

There is no any conflicts of interest between the authors.

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Authors and Affiliations

  1. Neonatal Intensive Care Unit, Zekai Tahir Burak Maternity Teaching Hospital, 06110, Hamamönü/Ankara, Turkey

    Sadık Yurttutan, Fuat Emre Canpolat, Mehmet Yekta Oncel & Ugur Dilmen

  2. Division of Neonatology, Department of Pediatrics, Inönü University School of Medicine, Malatya, Turkey

    Ramazan Ozdemir

  3. Department of Pathology, Ankara Etlik Ihtisas Training and Research Hospital, Ankara, Turkey

    Hatice Germen Unverdi

  4. Department of Physiology, Gulhane Military Medical Academy, Ankara, Turkey

    Bülent Uysal

  5. Division of Neonatology, Department of Pediatrics, Ankara University School of Medicine, Ankara, Turkey

    Ömer Erdeve

  6. Department of Pediatrics, Yıldırım Beyazıt University School of Medicine, Ankara, Turkey

    Ugur Dilmen

Authors
  1. Sadık Yurttutan
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  2. Ramazan Ozdemir
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  3. Fuat Emre Canpolat
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  4. Mehmet Yekta Oncel
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  6. Bülent Uysal
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Correspondence to Sadık Yurttutan.

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Yurttutan, S., Ozdemir, R., Canpolat, F.E. et al. Beneficial effects of Etanercept on experimental necrotizing enterocolitis. Pediatr Surg Int 30, 71–77 (2014). https://doi.org/10.1007/s00383-013-3415-4

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  • DOI: https://doi.org/10.1007/s00383-013-3415-4

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