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BMPR1A-mediated BMP1 signalling is disrupted in the cadmium-induced omphalocele in the chick model

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Abstract

Purpose

In the chick embryo, administration of cadmium (Cd) induces omphalocele phenotype. The earliest histological changes in this model are observed commencing at 4-h post treatment (4H). The molecular mechanisms by which Cd acts during the critical period of embryogenesis remain unclear. Bone morphogenetic proteins (BMPs) and their receptors (BMPRs) regulate many fundamental biological processes and are involved in various embryonic morphogenesis, including ventral body wall (VBW) formation. Homozygous BMP1 mutant mice cause VBW defects. It has been reported that BMPR1A conditional knockouts also exhibit omphalocele phenotype. We designed this study to test the hypothesis that gene expression levels of BMP1 and BMPR1A are downregulated during the critical period of embryogenesis in the Cd chick model.

Methods

After 60-h incubation, chick embryos were exposed to either Cd or saline and then harvested 1H, 4H and 8H. Chicks were divided into control (n = 24) and Cd (n = 24). RT-PCR was performed and differences between the two groups were tested statistically (significance was accepted at p < 0.05). Immunohistochemical study was also performed to evaluate those proteins expression/distribution.

Results

The gene expression levels of BMP1 and BMPR1A at 4H were significantly downregulated in Cd group compared to controls. Immunoreactivity of BMP1 and BMPR1A was also markedly decreased in Cd-treated embryos compared to controls.

Conclusion

Disruption of BMPR1A-mediated BMP1 signalling during the narrow window of early embryogenesis may interfere with normal VBW formation, causing omphalocele phenotype in the Cd chick model.

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Authors and Affiliations

  1. National Children’s Research Centre, Our Lady’s Children’s Hospital, Dublin 12, Ireland

    Takashi Doi & Prem Puri

  2. School of Medicine and Medical Science, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland

    Takashi Doi, Prem Puri, John Bannigan & Jennifer Thompson

Authors
  1. Takashi Doi
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  2. Prem Puri
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  3. John Bannigan
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  4. Jennifer Thompson
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Correspondence to Prem Puri.

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Doi, T., Puri, P., Bannigan, J. et al. BMPR1A-mediated BMP1 signalling is disrupted in the cadmium-induced omphalocele in the chick model. Pediatr Surg Int 27, 617–621 (2011). https://doi.org/10.1007/s00383-010-2842-8

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  • DOI: https://doi.org/10.1007/s00383-010-2842-8

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