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Development of an acute obstructive hydrocephalus model in rats using N-butyl cyanoacrylate

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Abstract

Purpose

Previous animal models of obstructive hydrocephalus that was frequently combined with subarachnoid inflammation are not suitable for investigating cerebrospinal fluid (CSF) flow between ventricles and cisterns in obstructive hydrocephalus. In this study, we attempted to develop a new animal model for obstructive hydrocephalus in rats sparing subarachnoid space using N-butyl cyanoacrylate (NBCA).

Methods

Hydrocephalus was induced in adult male Sprague–Dawley rats with NBCA (n = 15) or with kaolin (n = 10). For the NBCA model, a silicone tube was inserted through the foramen of Magendie into the fourth ventricle, into which 20 μL of a mixture of NBCA and ethiodized oil was injected. For the kaolin model, 100 μL of 20% kaolin solution was injected into the cistern magna. The rats in the NBCA and kaolin groups were sacrificed 3 and 21 days after surgery, respectively.

Results

Eleven rats in the NBCA group developed hydrocephalus (73.3%), with a 13.3% mortality rate. The kaolin group showed hydrocephalus in eight rats (80%), with a 20% mortality rate. The mean Evans’ indices were 0.37 ± 0.02, 0.45 ± 0.04, and 0.53 ± 0.09 in the control, NBCA, and kaolin groups, respectively (p < 0.05). There was no remarkable arachnoid adhesion or inflammatory change of the ventricular wall in the NBCA group.

Conclusions

The NBCA model seems to be a useful animal model for acute obstructive hydrocephalus with preserved subarachnoid CSF pathway. This model can be useful for studying CSF flow between ventricles and cisterns.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 20100026410).

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Authors and Affiliations

  1. Department of Neurosurgery, Chung-Ang University College of Medicine, 65-207 Hangang-ro 3, Yong San gu, Seoul, South Korea

    Yong Sook Park, Seung Won Park, Jong Sik Suk & Taik Kyun Nam

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  1. Yong Sook Park
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  2. Seung Won Park
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Correspondence to Seung Won Park.

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Park, Y.S., Park, S.W., Suk, J.S. et al. Development of an acute obstructive hydrocephalus model in rats using N-butyl cyanoacrylate. Childs Nerv Syst 27, 903–910 (2011). https://doi.org/10.1007/s00381-011-1398-9

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  • DOI: https://doi.org/10.1007/s00381-011-1398-9

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