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Investigation of maternal melatonin effect on the hippocampal formation of newborn rat model of intrauterine cortical dysplasia

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Abstract

Objectives

Cortical dysplasia is a cortical malformation resulting from any developmental defects during different periods of development. This study aims to investigate the hippocampal histopathological alterations in the neonates with cortical dysplasia due to the prenatal exposure to carmustine (1,3-bis (2-chloroethyl)-1-nitrosoure; BCNU) and the possible effects of prophylaxis with melatonin, a neuroprotective agent.

Methods

Wistar albino female rats were randomly divided into four experimental groups; control, melatonin-treated, BCNU-exposed and BCNU-exposed+melatonin-treated. Light microscopy and immunohistochemistry were carried out on the newborn hippocampus.

Results

Histopathology of hippocampus from the control and melatonin-treated groups showed continuity of migration and maturation as patognomonic signs of the normal newborn hippocampus. Hippocampal cortex from the newborns exposed in utero to BCNU showed the histology of early embryonic hippocampal formation with immunohistochemical increase in the number of nestin positive cells and decreases in the immunoreactivity of glial fibrillary acidic protein (GFAP) and synaptophysin. These findings indicate a significant delay in hippocampal maturation, migration, and synaptogenesis. Intrauterine treatment of BCNU-exposed rats with melatonin resulted in histopathological features almost similar to control group.

Conclusion

It has been concluded that cortical dysplasia induced by intrauterine BCNU administration results in delayed hippocampal maturation, which is successfully restored by intrauterine melatonin treatment.

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Acknowledgments

We wish to thank Mr Erdinç Yılmaz and Mr İsmail Zonguldak for their technical help.

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Authors and Affiliations

  1. Department of Histology and Embryology, Ege University School of Medicine, Bornova, Izmir, Turkey

    Meral Baka, Yiğit Uyanikgil & Utku Ateş

  2. Department of Paediatrics, Ege University School of Medicine, Bornova, Izmir, Turkey

    Nilgün Kültürsay

  3. Ege Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji AD, 35100, Bornova, Izmir, Turkey

    Yiğit Uyanikgil

Authors
  1. Meral Baka
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  2. Yiğit Uyanikgil
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  3. Utku Ateş
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  4. Nilgün Kültürsay
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Corresponding author

Correspondence to Yiğit Uyanikgil.

Additional information

This study was presented partially at the 9th World Congress of Perinatal Medicine that was held between 24–28 October 2009 in Berlin, Germany.

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Baka, M., Uyanikgil, Y., Ateş, U. et al. Investigation of maternal melatonin effect on the hippocampal formation of newborn rat model of intrauterine cortical dysplasia. Childs Nerv Syst 26, 1575–1581 (2010). https://doi.org/10.1007/s00381-010-1147-5

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  • DOI: https://doi.org/10.1007/s00381-010-1147-5

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