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Monitoring the growth effect of xenotransplanted human medulloblastoma in an immunocompromised mouse model using in vitro and ex vivo green fluorescent protein imaging

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Abstract

Introduction

Medulloblastoma (MB) is one of the most common malignant brain tumors in children. It is a radiosensitive tumor. At 5 years after radical surgical excision and craniospinal axis irradiation, the tumor-free survival rate is from 50 to 70% [Halperin EC, Constine LS, Tarbell NJ, Kun LE. Pediatric radiation oncology (2005)].

Case report

In this study, we established xenotransplanted human MB (hMB) cells — isochromosome 17q — in a severe combined immunodeficiency (SCID) mouse model. We further transduced green fluorescent protein (GFP) into hMB cells to evaluate these hMB cells grafted in SCID mice.

Results

The result of an ex vivo GFP imaging system showed that a small lesion of the third-week-hMB-transplanted graft presented “green” signals with a clear tumor margin before any tumor-related symptoms were noted. We also demonstrated that the tumor progression could be monitored by GFP imaging for up to 12 weeks post-transplantation.

Conclusions

This novel approach of GFP imaging assessment provides more accurate information of tumor status for experimental brain tumor studies. Because MB is sensitive to radiation and also response to chemotherapy, this SCID mouse model will be helpful for preclinical studies in the future.

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Acknowledgements

This study was supported by Stem Cell Project (94-396-4/5/6) of Taipei Veterans General Hospital, the Joint Projects of UTVGH (94P1-04/06/10), Yen-Tjing Ling Medical Foundation, and Molecular & Genetic Imaging Core/National Research Program for Genomic Research (NSC91-3112-P-075-001-Y).

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Authors and Affiliations

  1. Department of Education and Research, The Neurological Institute, Taiwan, Republic of China

    Shih-Hwa Chiou & Han-Tso Lin

  2. Department of Physical Medicine & Rehabilitation, The Neurological Institute, Taiwan, Republic of China

    Chung-Lan Kao

  3. Division of Pediatric Neurosurgery, The Neurological Institute, Taipei Veterans General Hospital and National Yang-Ming University, Taiwan, Republic of China

    Wen-Ser Tseng & Tai-Tong Wong

  4. Department of Nuclear Medicine, Taipei Veterans General Hospital and National Yang-Ming University, Taiwan, Republic of China

    Ren-Shyan Liu

  5. Institute of Anatomy and Cell Biology, National Yang-Ming University, Taiwan, Republic of China

    Han-Tso Lin, Chen-Fun Chung & Hung-Hai Ku

  6. Institute of Neuroscience, National Yang-Ming University, Taiwan, Republic of China

    Ching-Po Lin

  7. Institute of Clinical Medicine, National Yang-Ming University, Taiwan, Republic of China

    Shih-Hwa Chiou

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  1. Shih-Hwa Chiou
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  2. Chung-Lan Kao
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  3. Han-Tso Lin
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  4. Wen-Ser Tseng
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  5. Ren-Shyan Liu
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  6. Chen-Fun Chung
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  7. Hung-Hai Ku
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  8. Ching-Po Lin
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  9. Tai-Tong Wong
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Corresponding author

Correspondence to Tai-Tong Wong.

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Chiou, SH., Kao, CL., Lin, HT. et al. Monitoring the growth effect of xenotransplanted human medulloblastoma in an immunocompromised mouse model using in vitro and ex vivo green fluorescent protein imaging. Childs Nerv Syst 22, 475–480 (2006). https://doi.org/10.1007/s00381-005-0026-y

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  • DOI: https://doi.org/10.1007/s00381-005-0026-y

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