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Expression of interferon-g and interleukin-4 production in CD4+ T cells in patients with chronic heart failure

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Abstract

The prevalence of inflammation is high among patients with chronic heart failure (CHF). Reduced ejection fraction was associated with frequency of CD4+ T cells of leukocytes. Therefore, we investigated inflammatory cytokines of expression markers in CD4+ T cells in patients with CHF. Blood samples were obtained from 103 patients with CHF, from 83 patients with stable angina (SA), and from 57 controls. Interferon-γ (IFN-γ)-positive CD4+ T cells and interleukin-4 (IL-4)-positive CD4+ T cells were analyzed using 3-color flow cytometry. The frequency (%) of IFN-γ-positive CD4+ T cells increased in patients with CHF compared with those with SA and controls (CHF: 28.3 ± 13.8, SA: 23.50 ± 10.38, controls: 19.00 ± 7.45, P < 0.001). There was no significant difference in the frequency of IL-4-positive CD4+ T cells among the three groups. The frequencies of CD4+ T cells that stained for IFN-γ decreased from 32.37% ± 16.40% on admission to 26.91% ± 12.53% after 2 weeks in 26 patients with CHF. B-type natriuretic peptide (pg/ml) and high-sensitivity C-reactive protein (mg/dl) levels decreased from 251.7 ± 150.4 and 0.64 ± 0.78 on admission to 208.2 ± 166.4 and 0.36 ± 0.34 after 2 weeks in the 26 patients with CHF. We have demonstrated expression of IFN-γ production of CD4+ T cells during CHF. Prevention of unwanted T cell activation could represent a new target in the treatment of CHF.

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Authors and Affiliations

  1. Department of Cardiovascular Medicine, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto, 860-8556, Japan

    Takashi Fukunaga, Hirofumi Soejima, Koichi Sugamura, Yoko Oe, Tomoko Tanaka, Sunao Kojima, Tomohiro Sakamoto, Michihiro Yoshimura & Hisao Ogawa

  2. Department of Neuroscience Immunology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan

    Atsushi Irie & Yasuharu Nishimura

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  1. Takashi Fukunaga
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Correspondence to Hirofumi Soejima.

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Fukunaga, T., Soejima, H., Irie, A. et al. Expression of interferon-g and interleukin-4 production in CD4+ T cells in patients with chronic heart failure. Heart Vessels 22, 178–183 (2007). https://doi.org/10.1007/s00380-006-0955-8

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  • DOI: https://doi.org/10.1007/s00380-006-0955-8

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