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Carbon investment into mobilization of mineral and organic phosphorus by arbuscular mycorrhiza

Abstract

To overcome phosphorus (P) deficiency, about 80% of plant species establish symbiosis with arbuscular mycorrhizal fungi (AMF), which in return constitute a major sink of photosynthates. Information on whether plant carbon (C) allocation towards AMF increases with declining availability of the P source is limited. We offered orthophosphate (OP), apatite (AP), or phytic acid (PA) as the only P source available to arbuscular mycorrhiza (AM) (Solanum lycopersicum x Rhizophagus irregularis) in a mesocosm experiment, where the fungi had exclusive access to each P source. After exposure, we determined P contents in the plant, related these to the overall C budget of the system, including the organic C (OC) contents, the respired CO2, the phospholipid fatty acid (PLFA) 16:1ω5c (extraradical mycelium), and the neutral fatty acid (NLFA) 16:1ω5c (energy storage) at the fungal compartment. Arbuscular mycorrhizal (AM) plants incorporated P derived from the three P sources through the mycorrhizal pathway, but did this with differing C-P trading costs. The mobilization of PA and AP by the AM plant entailed larger mycelium infrastructure and significantly larger respiratory losses of CO2, in comparison with the utilization of the readily soluble OP. Our study thus suggests that AM plants invest larger C amounts into their fungal partners at lower P availability. This larger C flux to the AM fungi might also lead to larger soil organic C contents, in the course of forming larger AM biomass under P-limiting conditions.

Introduction

Phosphorus is the most limiting nutrient for plant growth after nitrogen (N), especially in soils from tropical ecosystems, where secondary minerals immobilize P (Johnston et al. 2019). As a consequence, almost 80% of plant species establish symbiotic associations with arbuscular mycorrhizal fungi (AMF) (Smith and Read 2008). This symbiotic relationship, being several hundreds of million years old (Lambers et al. 2009), explores a large volume of soil and does this at a lower cost as compared with roots. Such low-cost modes of P acquisition are expected to be favored by natural selection and during the assembly of plant communities by competition (Raven et al. 2018). One reason for the better cost to benefit ratio is that AMF hyphae represent the lower construction and maintenance costs for infrastructure (2–20-μm diameter), compared with root hairs (100–500-μm diameter) (Hodge 2017). The AMF are able to mobilize the otherwise poorly accessible phosphate ions beyond the P depletion zone (Osorio et al. 2017), and are responsible for nearly 80% of the plant P uptake (Douds and Johnson 2007).

Mycorrhizal benefits are greater under P-limiting soil conditions than in P-rich ones. In exchange for the P acquisition, AMF represent a major sink for recently fixed plant photosynthates (Nottingham et al. 2010), investing the host plant C into the fungal growth, respiration, and biological maintenance (Jansa et al. 2011). Photosynthetic rates are often higher in mycorrhizal than in non-mycorrhizal plants (Augé et al. 2016), as a result of improved plant nutrition, especially of P, in mycorrhizal plants (Gavito et al. 2019). The AM plants allocate 4–20% of the total C fixed into the AMF structures (Parniske 2008), and up to 5% of net photosynthesis is lost by hyphal respiration (Moyano et al. 2007). AMF may also act as a gateway for direct delivery of recent plant photosynthates to the hyphospheric microbes within hours (Kaiser et al. 2015), exhibiting an intimate relationship and suggesting a synergistic cooperation (Zhang et al. 2016).

Plant P acquisition strategies require photoassimilates as energy form (Li et al. 2019). In this regard, Turner (2008) hypothesized that the different soil P forms can be placed along a gradient of increasing energy investment required for their acquisition, since insoluble organic form (e.g., phytic acid, PA) and P-containing primary minerals (e.g., apatite, AP) have comparatively high pre-processing costs, even at high concentrations, and these costs may steeply rise, as concentration declines (Raven et al. 2018).

Apatite is the most important primary mineral containing P (Nezat et al. 2008). It accounts for over 95% of all P in the Earth’s crust, thus playing a vital role in providing P to all ecosystems (Ptáček 2016). The AMF may tap into the crystalline structure of the AP through the exudation of acids and mobilizing the released P (Ness and Vlek 2000), but there is not a single report on the acquisition costs.

Organic P constitutes 20–80% of the total P in the soil, with PA representing > 50% of the organic P (Shen et al. 2011). Turner (2008) identified PA as the P source most resistant to hydrolysis, and the metabolically most expensive source among the soil P compounds. In plants establishing an ectomycorrhizal association (ECM), C allocation into the extraradical hyphae increases proportionally at the absence of readily available P and/or under low P conditions (Smits et al. 2012); furthermore, ECM might play a critical role in the decomposition of organic P, essential to meet host plant P requirements and maintain the balance of soil labile P (Fan et al. 2018). We recently confirmed the ability of the cosmopolitan AMF R. irregularis DAOM 197198 to mobilize free PA and PA bound to the iron oxide goethite. Their mobilization proved to be the most expensive for the mycorrhized plant, in terms of C investment (Andrino et al. 2019). On the one hand, AMF may exudate their own acid phosphomonoesterase, to mineralize organic P forms and access P sources that non-mycorrhizal plants are unable to mobilize (Sato et al. 2015). On the other one, soil microbes may cooperate intimately with the AMF by providing inorganic P released by the decomposition of organic sources, in exchange for C exuded by the hyphae. But the mechanisms by which these exudates trigger the process of P mineralization in bacteria are still unknown (Zhang et al. 2018).

In the present study, we aim at identifying some factors controlling the interrelation of the energy investment (C) by the model tomato plant (Solanum lycopersicum L.) to mobilize the differently accessible P pools in the soil, by a cosmopolitan AMF such as R. irregularis. For that, we will employ the same dual-compartment system, where exclusively the AMF hyphae have access to the different P sources in a fungal compartment (FC) as in the experiment of Andrino et al. (2019). In the present study, we will quantify the full C budget of the mycorrhizal symbiosis by measuring the respired CO2, total organic C accumulated, and fungal biomass at the FC, and assess the C-P stoichiometry to acquire P from primary minerals such as AP, as opposed to the mobilization of free orthophosphate (OP) and PA. Furthermore, we will investigate the fate of the newly assimilated photosynthates in the plant-AMF-microbiota continuum, attempting to understand whether plant and fungus react in a coordinated manner, when mobilizing P sources with differing accessibility. We hypothesize that less accessible P sources, namely PA and AP, will cause larger photoassimilate investments by the host plant and will result in differing trading costs per P unit in comparison with easily accessible P sources such OP.

Material and methods

To compare the acquisition costs of incorporating P from differently accessible sources through the mycorrhizal pathway, we conducted two experiments. Experiment 1 allowed us to test the ability of the AMF to mobilize P from the different sources offered at the FC during a time-course experiment over 112 days, but more importantly, to estimate whether there is a differentiated plant C investment into the AMF. Experiment 2 was carried out to compare the current acquisition costs in the form of recent photoassimilates using stable isotope 13C at the specific time point elucidated in the experiment 1. This was done only at treatments, where it was possible to quantify the incorporation of the different P sources.

Experiment 1

Solanum lycopersicum L. inoculation with Rhizophagus irregularis DAOM 197198

A system of one plant and one fungus was selected to test our hypotheses. One-one models are limited to tackle with the diversity of possible responses that a natural system can offer, while they are excellently suited for elucidating processes and understanding the underlying mechanisms developed by single AMF species (Zou et al. 2015). Tomato seeds var. Moneymaker (Volmary GmbH, Münster, Germany) were surface-sterilized with H2O2 (5%, 10 min), rinsed with sterile distilled water (3×) and germinated on the surface of a moistened filter paper inside a petri dish (3 days, 27 °C). The R. irregularis DAOM 197198 model organism (Daubois et al. 2016) was selected as mycorrhizal symbiont due to its ubiquitous occurrence and adaptation to agricultural practices (Köhl et al. 2016). The inoculum of the strain DAOM 197198 consisting of spores and root fragments (Symplanta GmbH & Co. KG. Darmstadt, Germany) was reproduced in a trap plant culture (Brundrett et al. 1996). Germinated tomato seeds were transplanted into 75-ml pots QP96 (HerkuPlast Kubern GmbH, Ering, Germany) with 0.4 g containing 35–50 AMF propagules (roots, spores, hyphae) and 70-ml autoclaved and acid washed quartz sand, while non-mycorrhizal controls (M−) received 0.4 g of non-inoculated chopped roots. The quartz sand was used as plant and fungal growth substrate due to its suitability for the AM mycelium development (Olsson and Johansen 2000). Mycorrhizal and control plants were grown at a greenhouse for 4 weeks (photoperiod 16/8 h light/dark, temperature 24/20 °C light/dark, 50–60% relative air humidity, photon flux density of 175–230 μmol m−2 s−1). Plants were watered every day with 10 ml deionized water, and on alternate days the pots were fertilized with 5 ml low P (0.32 mM) modified Long Ashton nutrient solution pH 6.5 (Hewitt 1966). A quality control was carried out before the transplantation into the mesocosm, to monitor whether the AM plants were mycorrhized. For this, tomato mycorrhizal roots from AM plants and controls were stained using the ink and vinegar staining technique for visualizing the AMF colonization (Vierheilig et al. 1998). The percentage of mycorrhization was higher than 60% for plants transplanted into the mesocosms.

Temporal dynamics

Four-week-old mycorrhized tomato seedlings were planted into each mesocosm and placed at a greenhouse (photoperiod 16/8 h light/dark; temperature 24/20 °C light/dark; 50–60% relative humidity; photon flux density of 175–230 μmol m−2 s−1). Once a week, mesocosms were rotated within the greenhouse to achieve homogeneous growth conditions. To observe the temporal dynamics on the P acquisition by the AM plants and the controls, they were sampled five times within 112 days. The first sampling time was at the day of transplanting (day 0) to determine initial plant P content (n = 5), followed by four sampling times at days 35, 56, 86, and 112. At each sampling time, three biological replicates of each treatment were sampled.

The mesocosms consisted of a plant compartment (PC) and a FC, into which only the AMF hyphae were able to grow and mobilize the P sources offered there (Fig. 1a). Between both compartments, two barriers separated mycorrhizal roots and mycelium: a 20-μm pore size polyamide mesh (Franz Eckert GmbH, Waldkirch, Germany) (Watkins et al. 1996; Fitter et al. 1998) and a polytetrafluoroethylene (PTFE) membrane with a pore size of 5–10-μm (Pieper Filter GmbH, Bad Zwischenahn, Germany). AMF hyphae exhibit a high degree of plasticity in terms of its architecture (Püschel et al. 2020). AM arterial hyphae are much finer than roots at 20–30 μm, and their fine distal absorptive hyphae are only about 2–7-μm in diameter according to Leake and Read (2017). The PTFE membrane allowed the AM hyphae to cross but did not allow mass flow and diffusion of ions to the PC; thus, sources were only accessible to the AM plant via the hyphae (Mäder et al. 1993).

Fig. 1
figure 1

Scheme of mesocosms containing an arbuscular mycorrhizal plant in the left compartment and a fungus in the right compartment, also containing one of the three different P forms: orthophosphate in solution as readily available P form, apatite as mineral P source, and hytate as organic P source or a blank with no P. The plant compartment is separated from the fungal compartment by a double-mesh system, so that only the mycelium is able to access to the different P sources. (a) The fungal compartment of the mesocosms is connected to the EGM-4 infrared gas analyzer for CO2 measurement (experiment 1). (b) Scheme of the mesocosms setup used to measure the CO2 content and the δ13CO2 ratio respired at the fungal compartment. The gas bags were filled with the air respired from the fungal compartment and then were measured in the Picarro G1101-I (experiment 2). Abbreviations are OP, orthophosphate; AP, apatite; PA, phytic acid; M+, mycorrhizal plant with no P

Phosphorus sources offered at the fungal compartment

Three P sources were offered at the fungal compartment. Orthophosphate (OP) was used as inorganic and readily available source in the form of KH2PO4. Phytic acid (PA) (Sigma-Aldrich, Taufkirchen, Germany) was chosen as the organic source. A total content of 10 mg P was applied in the form of OP or PA and dissolved in 16 ml autoclaved MilliQ water and mixed with 60 g autoclaved acid washed quartz sand placed inside each fungal compartment. Ten milligram P content was decided on the basis to sustain the plant P necessities during the next 3 months after planting the seedlings into the mesocosms. As third P source, we have chosen AP as a primary mineral source (2-mm diameter) (Krantz Company, Bonn, Germany). An average P content of 18.72% within the AP was characterized using a field emission scanning electron microscope equipped with an energy-dispersive spectrometer (Phillips/FEI XL-30, Hillsboro, Oregon, USA). Easily available P in water was determined for the AP by shaking 1 g of the mineral with 30 ml of autoclaved MilliQ water pH 7 (n = 3) in a 50-ml falcon test tube. The tubes were placed on an orbital shaker at 250 rpm for 7 days to determine maximum amount of P released from the AP in MilliQ water. The centrifuged supernatant (3000×g, 15 min) was filtered through a 0.45-μm syringe filter (PVDF) and 1 ml was mixed with 1 ml 30% HNO3, and the mixture was filled up to 10 ml with MilliQ water. Total P concentration was measured by ICP-MS Agilent 7500C (Agilent Technologies Ireland Ltd., Cork, Ireland) and resulted in 0.03 mg ± 0.004 mg P g−1 AP in the MilliQ water. An amount of 3 g AP containing 562 mg total P and 0.09 mg easily available P was added to 60 g quartz sand and 16 ml autoclaved MilliQ water. Apatite was added in a greater amount as compared with OP and PA, to know whether AMF could exclusively mobilize more P than the easily available fraction. Apatites are mostly of sedimentary origin (Arif et al. 2017), and the different formation processes have an impact in their physical, chemical and crystallographic properties and consequently in their effectiveness as a P source (Arcand and Schneider 2006). For this reason, it was decided that AP should be in excess, thus preventing P from being a limiting nutrient since the water available fraction was already very low (0.03 mg P/g AP). Furthermore, the other two P sources OP and PA were homogeneously distributed throughout the FC, having been dissolved in ultra-pure water, while 2-mm AP particles were mixed with the quartz sand. Thus, AP was also supplied in excess, to increase the probability of the hyphae exploiting the 2-mm granules mixed in the FC.

The water content at the fungal compartments was checked once a week and maintained with autoclaved MilliQ water at a volumetric water content of 19% (equivalent to field capacity) with the aid of a time-domain reflectometry probe Trime Pico connected to a Trime-FM version P2 (Imko Micromodultechnik GmbH, Ettlingen, Germany).

Plant P content

At each of the five sampling times, shoots and roots were air-dried (70 °C, 48 h), weighed, and ball-milled (Retsch GmbH, Haan, Germany). An aliquot of the grinded shoot and root samples was incinerated at 480 °C for 8 h, digested with 1 ml 30% HNO3, filtered through a 0.45-μm syringe filter (PVDF) and analyzed by ICP-MS (Agilent 7500C, Agilent Technologies Ireland Ltd., Cork, Ireland). The amount of total P incorporated by the AM plant over time was calculated as the difference of the total P content at each sampling time and the average total P content initially present in the transplanted plants at day 0 (n = 5).

Carbon dioxide production and organic C in the fungal compartment

At each sampling time, 2 g from each fungal compartment were air-dried (70 °C, 48 h), weighed, and ball-milled. The total C content was determined on an Elementar vario Isotope cube C/N analyzer (Elementar GmbH, Hanau, Germany) and was considered organic C (OC), as there was no inorganic C in the fungal compartment. For the calculation of the total OC in the fungal compartment, the total C content (mg g−1) was multiplied by the total weight of substrate in the fungal compartment. The CO2 production rate (mmol CO2 m2 h−1) was measured in the fungal compartment with an EGM-4 infrared gas analyzer (PP-systems, Hitchin, UK), a closed dynamic system (Vermue et al. 2008), during the whole experiment at 5 days per week (Fig. 1a). The cumulative CO2-C production was interpolated using a cubic spline function and calculated in milligram at each fungal compartment for each sampling day (Gentsch et al. 2018). The cumulative CO2-C/OC ratio was used as a proxy of the metabolic activity at the fungal compartment (Eq. 1). In the case of those AM plants mobilizing PA, when calculating the CO2-C to OC ratio, 3.84 mg C was subtracted from the total OC found in the FC, as that is the amount of C present in the 37.72 mg of PA containing 10 mg P.

$$ \mathrm{C}{\mathrm{O}}_2-\mathrm{C}/\mathrm{OC}=\frac{\mathrm{cumulative}\ \mathrm{C}{\mathrm{O}}_2-\mathrm{C}\ \left(\mathrm{mg}\right)}{\mathrm{organic}\ \mathrm{C}\ \left(\mathrm{mg}\right)} $$
(1)

R. irregularis: biomass and energy storage estimation

The fatty acid composition of R. irregularis DAOM 197198 includes molecules from C16:0 to C22:2 with 16:1ω5 as major fatty acid (Wewer et al. 2014). The PLFA 16:1ω5c is a biomarker to estimate the amount of extraradical AMF mycelia (Olsson and Johansen 2000). The NLFA 16:1ω5c biomarker estimates the amount of energy the fungus allocates for storage and is found in vesicles, spores, and extraradical mycelium (Bååth 2003). It is metabolized in the mycelium through the glyoxalate cycle providing the major fungal respiratory substrate (Van Aarle and Olsson 2003).

Lipids were extracted twice from 16 g of the different fungal compartments, belonging to different treatments and harvesting points, using a chloroform-methanol-citrate buffer 0.15 M pH 4 (1:2:0.8 v:v:v). Extracts were fractionated by solid-phase extraction with activated silica gel (Sigma-Aldrich, pore size 60 Å, 70–230 mesh) into neutral lipid fatty acids (NLFA), glycolipids, and phospholipid fatty acids (PLFA) by elution with 5 ml of chloroform, 20 ml of acetone, and 20 ml of methanol, respectively. The PLFA and NLFA samples were exposed to mild alkaline methanolysis. Transformation of phospholipids and neutral lipids into free fatty acid methyl esters (FAMEs) was based on Frostegård et al. (1991) with modifications by Bischoff et al. (2016). FAMEs were separated by gas chromatography using an Agilent 7890A GC system (Agilent Technologies Ireland Ltd., Cork, Ireland) equipped with a 60-m Zebron capillary GC column (0.25-mm diameter and 0.25-μm film thickness; Phenomenex, Torrance, California, USA) and quantified with a flame ionization detector, using He as carrier gas. Nonadecanoic acid (25 μg) and glyceryl tridodecanoate (25 μg) were used as internal standards during the extraction, and tridecanoic acid methyl ester (15 μg) was added to each sample and standard before GC analysis as a recovery standard. For the fatty acid identification, the relative retention times were compared with that of the internal standard using the Agilent GC ChemStation (B.03.02.341) software. In our study, PLFA and NLFA 16:1ω5c were measured to estimate the biomass and energy storage of R. irregularis in the fungal compartment, respectively (Stumpe et al. 2005). Three ratios were calculated to evaluate the C-P trading costs between the AMF and the host plant to acquire the different P sources. The ratios between 16:1ω5c PLFA to plant P uptake (Eq. 2) and NLFA to plant P uptake (Eq. 3) served to estimate the investment made by the tomato plants into their fungal partner in either biomass or energy storage to obtain P from each source, respectively. The ratio between NLFA 16:1ω5c and PLFA 16:1ω5c (Eq. 4) was used as an index for the R. irregularis growth strategy of (Rinnan and Bååth 2009). A high NLFA 16:1ω5c to PLFA 16:1ω5c ratio denotes C allocation to storage products in form of neutral lipids, in order to meet the catabolic needs of the fungus. Additionally, this ratio allows to discriminate between bacterial and fungal origin of the 16:1ω5c fatty acid. When the NLFA 16:1ω5c to PLFA 16:1ω5c was > 1, the fatty acid originated from AM fungi and not from bacteria (Cozzolino et al. 2016).

$$ \mathrm{PLFA}\ 16:1\upomega 5\mathrm{c}/\mathrm{P}=\frac{\mathrm{P}\mathrm{LFA}\ 16:1\upomega 5\mathrm{c}\ \left(\mathrm{total}\ \upmu \mathrm{g}\ \mathrm{per}\ \mathrm{fungal}\ \mathrm{compartment}\right)}{\mathrm{P}\ \mathrm{incorporated}\ \left(\mathrm{mg}\ \mathrm{per}\ \mathrm{plant}\right)} $$
(2)
$$ \mathrm{NLFA}\ 16:1\upomega 5\mathrm{c}/\mathrm{P}=\frac{\mathrm{NLFA}\ 16:1\upomega 5\mathrm{c}\ \left(\mathrm{total}\ \upmu \mathrm{g}\ \mathrm{per}\ \mathrm{fungal}\ \mathrm{compartment}\right)}{\mathrm{P}\ \mathrm{incorporated}\ \left(\mathrm{mg}\ \mathrm{per}\ \mathrm{plant}\right)} $$
(3)
$$ \mathrm{NLFA}/\mathrm{PLFA}=\frac{\mathrm{NLFA}\ 16:1\upomega 5\mathrm{c}\ \left(\mathrm{total}\ \upmu \mathrm{g}\ \mathrm{per}\ \mathrm{fungal}\ \mathrm{compartment}\right)}{\mathrm{PLFA}\ 16:1\upomega 5\mathrm{c}\ \left(\mathrm{total}\ \upmu \mathrm{g}\ \mathrm{per}\ \mathrm{fungal}\ \mathrm{compartment}\right)} $$
(4)

Experiment 2

The decision on the sampling day for experiment 2 was made on the basis of the results from experiment 1. The ideal labeling point for experiment 2 was defined at the moment when all treatments showed the maximum P incorporation from the different P sources. The same growing conditions described in experiment 1 were replicated for two identical groups of plants, which were grown until the date determined at experiment 1. The first group of plants was pulse-labeled with 13C stable isotope and the second one was not labeled as control. Each identical group contained three biological replications for each P source and a mycorrhized control plant without P. The labeling experiment aimed at determining the amount of freshly assimilated C invested on the one hand, into leaves, shoots, roots, substrates of the plant compartment, and on the other one, into the respired CO2 and the different microbial communities at the fungal compartment.

Photosynthetic rates

The photosynthetic capacity of the tomato plants was measured with the LI-6400 (Licor, Lincoln, NE, USA) on recently fully expanded third or fourth leaves from top, 1 day before the 13CO2 pulse labeling. The photosynthetic rate was used to test the effect of the different P sources on the host's carbohydrate metabolism, as P deficiency may cause a rapid decrease in photosynthetic rates (Maathuis 2009). Values were recorded at 22 °C in the leaf cuvette, a photon flux density of 1000 μmol m−2 s−1, approx. 50% relative humidity, airflow rate of 400 μmol s−1, an external CO2 concentration of 360 ppm, and the CO2 mixer to reference 400 ppm.

13C pulse labeling: 13C incorporation into respired CO2, plant tissues, and substrates

Mesocosms were placed into a phyto-chamber (20–22 °C, 50–60% relative air humidity, photon flux density of 195 μmol/m/s) and connected to a multi-valve system through a gas inlet and outlet at the fungal compartment to test for air leaks. Purified air with a CO2 concentration of 415 ppm and a δ13C ratio of − 11‰ (Zero Air generator UHP-35ZA-S, Parker Hannifin, Cleveland, USA) was then pumped for 120 min through the fungal compartment (4 ml min−1), and the stability of the flow rate was checked every 10 min. Once the absence of leaks in the gas circuit between the mesocosms and the valve system has been verified, 12 sampling bags (three bags per treatment) were connected to the programmable valve system and began filling for the next 120 min (Fig. 1b and S2). After all bags were filled, they were measured sequentially at the Picarro G1101-I cavity ring-down spectrometer (Picarro Inc., Sunnyvale, USA) to determine the 13CO2 abundance in the respired CO2 of each mesocosm. As these samples were taken before labeling, the corresponding values were defined as the background.

Thereafter, all mesocosms were brought to a labeling box (60 × 120 × 50 cm, H × W × D) with forced ventilation (Fig. S2) to promote homogeneous 13CO2 distribution during the pulse labeling. At the moment of closing the box, the internal CO2 concentration was 452 ppm had a δ13C ratio of − 12.23‰. Another identical set of mesocosms was reserved to determine the natural abundances in the plant tissues and the substrates of both compartments during the course of the experiment. The labeling box was placed inside a greenhouse (20 °C, 50–60% relative air humidity, photon flux density of 195 μmol m−2 s−1). The 13CO2 tracer used for pulse labeling consisted of 2 g Ca13CO3 (99 atom% 13C) (Sigma-Aldrich, Steinheim, Germany). The labeling box was closed at 10:00 h, and the tracer was dissolved in 1 M H2SO4 injected through the wall of the chamber. At 12:00 h, the labeling was stopped by opening the box and moving the labeled mesocosms to another room.

The CO2 concentration and its 13C signature in the fungal compartment was determined by a Picarro G1101-I (Picarro Inc., Sunnyvale, USA) (Fig. 1b and S2). To allow for a higher sampling intensity, a programmable valve system was developed to simultaneously collect a set of samples and then measure them consecutively. Gas samples were collected in 0.6-l gas sampling bags Tedlar® PLV with Thermogreen® LB-2 Septa (Sigma-Aldrich, Steinheim, Germany). Different pre-tests were carried out to find out the optimum sample volume for the Picarro device to obtain a steady measurement. It was found that at least 300 ml per bag were sufficient for a 10-min continuous measurement. The mesocosms were connected to the programmable valve system, and the airflow was adjusted to 4 ml min−1 for each of the 12 mesocosms. Once the airflow through each mesocosm was steady, the first batch of gas bags was connected from 13:00 to15:00 h (Fig. S2) as described for background sampling, followed by six other sampling times, covering a total of 25.5 h since the end of the 13CO2 pulse labeling (Fig. S2).

Leaves, shoots, roots, plant, and fungal compartment substrates were sampled 27 h after the labeling finished. The samples were freeze-dried and ball-milled, and aliquots were weighed into tin capsules for isotope ratio mass spectrometry analysis. The content of total C and the δ13C ratio in the samples was determined with the elemental analyzer Isotope cube (Elementar GmbH, Hanau, Germany) connected to an Isoprime 100 isotope ratio mass spectrometer (Elementar GmbH, Hanau, Germany) via a continuous flow inlet using helium (99.999% purity; Linde, Munich, Germany) as carrier gas. Calibration was carried out with certified solid standards from the International Atomic Energy Agency (IAEA, Vienna, Austria) sucrose (IAEA-CH-6), cellulose (IAEA-CH-3), and caffeine (IAEA-600). The enrichment of 13C was reported as atom % of 13C (at%13C), which was calculated with Eq. 5 for all plant, substrate, and gas samples,

$$ \mathrm{at}\%13\mathrm{C}\kern0.5em =\frac{\left(100\ \mathrm{x}\ \mathrm{AR}\ \mathrm{x}\ \left(\frac{\updelta 13\mathrm{C}}{1000+1}\right)\right)}{1+\left(\mathrm{AR}\ \mathrm{x}\ \left(\frac{\updelta 13\mathrm{C}}{1000+1}\right)\right)} $$
(5)

where AR represents the absolute ratio of 13C:12C relative to the Vienna PeeDee Belemnite standard (0.011182) and δ13C is the value in calibrated ‰ (Slater et al. 2001).

The proportion of freshly assimilated C (%CF) in the different pools (plant tissues, substrates, and respired CO2) was calculated based on a two-pool mixing model as given in Eq. 6. Here, at%13C is the 13C enrichment in the labeled (L) or unlabeled (UL) treatment, or the tracer (T), respectively.

$$ \%\mathrm{CF}\kern0.5em =\left(\frac{\mathrm{at}\%13\mathrm{CL}-\mathrm{at}\%13\mathrm{CUL}\ }{\mathrm{at}\%13\mathrm{CT}-\mathrm{at}\%13\mathrm{CUL}}\right)\times 100 $$
(6)

To calculate the content of freshly assimilated C (CF) in each sample (plant, substrate, gas), the %CF (calculated with Eq. 6) was divided by 100 and the result was multiplied by the total C content of the labeled sample (L) (Eq. 7):

$$ \mathrm{CF}\ \left(\mathrm{mg}\ \mathrm{or}\ \upmu \mathrm{g}\right)=\left(\frac{\mathrm{at}\%13\mathrm{CF}}{100}\right)\times \mathrm{CL} $$
(7)

All the freshly assimilated C contents (CF) were summed up, and the relative allocation of fresh C to the different pools (plant, substrate, gas) was calculated for each treatment.

13C PLFA and 13C NLFA analysis

Phospholipids and neutral lipids were extracted, saponified to the respective free fatty acids, and derivatized following the protocol described for experiment 1. A set of external standards (multi-standard) consisting of 17 fatty acids belonging to different microbial groups (Table 1) and internal standards (Sigma-Aldrich, Taufkirchen, Germany) were derivatized and measured together with the PLFA and NLFA fungal compartment samples. The 13C enrichment and the concentration in each FAME were measured in triplicate with an Agilent 7890A GC system (Agilent Technologies Ireland Ltd., Cork, Ireland) coupled with an Isoprime 100 IRMS (Elementar GmbH, Hanau, Germany) via a combustion interface and a continuous flow inlet using helium (99.999% purity Linde, Germany) as gas carrier. The area below each chromatogram peak and the δ13C values of the 17 substances plus internal standards were processed with the IonOS 2.0 software (Isoprime, Elementar GmbH, Hanau, Germany) to calculate the concentrations and the at%13C, respectively, of the different PLFA and NLFA biomarkers within the multi-standard solution and the samples.

Table 1 List of analyzed fatty acid biomarkers and their associated microbial group

The corrected PLFA and NLFA δ13C values were calculated to at%13C using Eq. 5. The proportion of freshly assimilated C into each PLFA or NLFA sample (%CF_PLFA or %CF_NLFA) was calculated using a two-pool mixing model explained in Eq. 6. To calculate the content of freshly assimilated C into PLFA or NLFA biomarkers (CPLFA or CPLFA) of each sample, the %CF_PLFA or %CF_NLFA (calculated with Eq. 6) was divided by 100, and the result was multiplied by the total PLFA or NLFA content of the labeled sample (L) and the total C content within each PLFA or NLFA molecule (Eq. 8),

$$ \mathrm{CPLFA}\ \left(\upmu \mathrm{g}\ \mathrm{FC}\right)=\left(\frac{\%\mathrm{CF}\_\mathrm{PLFA}}{100}\right)\times \left(\frac{\mathrm{M}\left(\upmu \mathrm{g}\ \mathrm{PLFA}\ \mathrm{FC}-\mathrm{C}\right)}{\mathrm{M}\left(\upmu \mathrm{g}\ \mathrm{PLFA}\ \mathrm{FC}\right)}\times \upmu \mathrm{g}\ \mathrm{PLFA}\mathrm{L}\right) $$
(8)

where 13CPLFA (μg FC) is the freshly assimilated C into PLFA or NLFA biomarkers, M(PLFA-C) is the molar mass of the C in the PLFA or NLFA molecule, and M(PLFA) is the molar mass of the PLFA or NLFA molecule. Once the freshly assimilated C contents were obtained in each of the biomarkers for each treatment, they were grouped according to the microbial group to which they belong (Table 1).

Leaf areas

The total leaf area (cm2) for each plant was measured with a LI-3100C (Licor, Lincoln, NE, USA) after finishing the pulse-chase labeling experiment. Deficiency in P nutrition has been recognized as an important factor reducing the size of the leaf area and consequently limiting light interception and the photosynthetic capacity of leaves (Rodríguez et al. 1998).

Data analysis

For both experiments, normality of the data was verified with the Shapiro-Wilk’s test and homogeneity of variances using the Levene’s test. One-way ANOVA analysis and the Duncan post hoc test was employed for mean separation (p < 0.05) between the different P sources offered at the fungal compartment for the different variables at each time point. Data analysis was performed using SPSS v.24 for Windows (IBM Corporation 2016).

Results

Experiment 1

P derived from the all three sources was incorporated into the different AM plants, starting from day 35 and lasting until the end of the experiment at day 112 (Fig. 2). Control mycorrhizal plants without access to a P source (M+) did not show any P incorporation. No significant differences were observed in P incorporation on day 35, but there were significant differences on day 56 for AM plants accessing PA. At day 84, all AM plants with access to a P source exhibited the largest P incorporation without a significant difference between the treatments regarding P uptake, and the mineral P source yielded greater amount than the 0.09 mg P available by the pure dissolution of AP. At day 112, the inorganic P forms OP and AP facilitated significantly more P for plant growth than for PA (Fig. 2). Furthermore, shoot and root biomass (Fig. S1) exhibited that control plants (M+) show a tendency to increase root biomass, contrary to the shoots at days 84 and 112, compared with the other three treatments with access to a P source.

Fig. 2
figure 2

Phosphorus incorporated into the arbuscular mycorrhizal plant along the time-course experiment (mean values ± standard error, n = 3). No P was incorporated in the M+ treatment. Within each P source, treatments with significant differences are indicated by different small letters (p < 0.05) as analyzed by one-way ANOVA. Abbreviations are OP, orthophosphate; AP, apatite; PA, phytic acid; M+, mycorrhizal plant with no P

The ratio of cumulative CO2-C production (mg) to OC content (mg) in the fungal chamber was significantly larger for those AM plants that had access to AP during the experiment, as compared with that of OP, PA, and the M+ control (Fig. 3).

Fig. 3
figure 3

Ratio of respired cumulative CO2-C (mg) and the accumulated organic C (mg) for the fungal compartment containing the different P sources (mean values ± standard error, n = 3). Within each P source, treatments with significant differences are indicated by different small letters (p < 0.05) as analyzed by one-way ANOVA. Abbreviations are OP, orthophosphate; AP, apatite; PA, phytic acid

Those AM plants that incorporated P from PA showed higher PLFA and NLFA 16:1ω5c to P ratios than the OP and AP treatments at days 35 and 84 (Fig. 4a and b). At day 112, only the PLFA 16:1ω5c to P ratio was significantly larger for AP and PA as compared with the OP. With incubation time, at days 84 and 112, the NLFA 16:1ω5c to PLFA 16:1ω5c ratio increased for all AM plants mobilizing a P source in the fungal compartment in contrast to the M+ control (Fig. 4c).

Fig. 4
figure 4

Ratios of PLFA 16:1ω5c to P incorporated within the tomato plant (a), of NLFA16:1ω5c to P incorporated (logarithmic scale) within the tomato plant (b), and of NLFA 16:1ω5c to PLFA 16:1ω5c (logarithmic scale) from the different P sources along the time-course experiment (mean values ± standard error, n = 3). Within each P source and day, treatments with significant differences are indicated by different small letters (p < 0.05) as analyzed by one-way ANOVA. Abbreviations are OP, orthophosphate; AP, apatite; and PA, phytic acid

Experiment 2

Since AM plants with access to a P source exhibited the largest P incorporation and did not show any significant differences in the amount of P incorporated from each source at day 84, a new set of plants was labeled on the same day for comparing the current costs of P acquisition from the different sources.

In case of respired 13CO2 derived from freshly assimilated C (μg) measured in the fungal compartment (Fig. 5), the M- control, without mycorrhiza and without P, showed a non-constant 13CO2 respiration over the 25.5 h pulse-chase experiment. In contrast, the OP and M+ treatments exhibited an increasing content of 13C (μg) in the respired CO2 8 h after the pulse labeling. The treatment containing AP exhibited significantly larger respired 13CO2 (μg) as compared with OP, M+, and M− from the ninth hour onwards. In the case of PA treatment, there were significant differences to the other treatments after the first 3 h of the pulse-chase experiment. The AP and PA treatments kept a steadily increasing trend regarding the respired 13CO2 (μg) which lasted until the end of the experiment (Fig. 5). Moreover, the sum of 13CO2 respired over 25.5 h monitored was significantly largest in case of PA, in comparison with treatments containing OP, AP, or M+ (Fig. 6).

Fig. 5
figure 5

Cumulative CO2 respiration from the fungal compartment (μg) derived from the freshly assimilated C after the 13C pulse labeling. Mean values and standard errors of the respired CO2 (μg) of three independent mesocosms at different time points for each treatment. Within each P source, treatments with significant differences are indicated by different small letters (p < 0.05) as analyzed by one-way ANOVA. Abbreviations are OP, orthophosphate; AP, apatite; PA, phytic acid; M+, mycorrhizal plant with no P; M−, non-mycorrhizal plant

Fig. 6
figure 6

Scheme containing the results of parameters measured before (photosynthetic capacity), during (freshly assimilated C), and after (leaf area) the 13C pulse labeling experiment of the different mesocosms. The photosynthetic capacity of the plants that accessed the different P sources immediately before the 13C pulse labeling is shown on the lower left side, the leaf area for the different treatments is shown on the upper left side, and the freshly assimilated C at the different plant organs, fungal compartment (Fc), and the cumulative CO2 respiration from the fungal compartment for the different treatments 24 h after the 13C pulse-chase labeling is shown on the right side. In bold letters inside each colored cell, the mean value and the standard error is shown (n = 3) for each parameter. Between brackets, the mean percentage of freshly assimilated C into the plant organs, the fungal and plant compartments (Pc), and the cumulative CO2 respiration is shown. Within each P source, treatments with significant differences are indicated by different small letters (p < 0.05) as analyzed by one-way ANOVA. Abbreviations are OP, orthophosphate; AP, apatite; PA, phytic acid; M+, mycorrhizal plant without P

Twenty-seven hours after labeling, the largest 13C content (mg) in the leaves was found in the AM plants accessing a P source in comparison with the M+ control. AM plants mobilizing OP and PA showed the largest 13C content (mg) in leaves, followed by AM plants with access to AP in the fungal compartment. Furthermore, the 13C content (mg) in the leaves represented around 60% of the total freshly assimilated C in case of AM plants mobilizing OP, which is more than for all others. In addition, AM plants with access to OP exhibited larger leaf areas and photosynthesis rates than the PA, AP, or M+ treatments. In the shoot tissues, the largest 13C content (mg) was found in the AM plants that accessed PA in comparison with treatments containing OP, AP, or M+ (Fig. 6). In case of the root samples, AM plants mobilizing AP over their mycorrhizal partner exhibited a larger 13C content (mg) as compared with those mobilizing PA, OP, or M+. The 13C content (mg) in the plant and fungal compartment substrates derived from freshly assimilated C was larger in all the AM plants that accessed a P source in comparison to the M+ control (Fig. 6).

Among the 17 studied PLFA biomarkers, only 11 were labeled with 13C (14:0, a15:0, i15:0, 15:0, 16:1w7c, 16:1w5c, 17:0, 18:2w6, 9, 18:1w9c, 18:1w7c, 18:0). The PLFA AMF biomarker contained more than 96% of 13C derived from freshly assimilated C within the PLFA biomarkers for all AM plants accessing a P source, in contrast to the M+ test with only 84% assimilated 13C into the AMF biomarkers (Fig. 7a). For the NLFA samples, only the AMF biomarker (16:1w5c) showed 13C enrichment. The 13C contents (μg) in the AMF biomarkers differed between the treatments; those treatments with access to PA, AP, and OP showed larger 13C contents (μg) compared with the M+ treatment (Fig. 7a). The relative amounts of recently assimilated C in the general biomarkers of bacteria (B) were not plotted in Fig. 7b, as they were up to an order of magnitude lower than the rest of the bacterial markers. The Gram-positive (B+) and Gram-negative (B−) PLFA biomarkers showed a minor percentage of 13C (< 0.3% B+; < 3.4% B−) derived from freshly assimilated C for all treatments containing a P source when compared with the M+ treatment (7.4% B+; 8.8% B−) (Fig. 7b). The NLFA AMF biomarkers exhibited the largest content of 13C (μg) derived from freshly assimilated C for the PA treatment followed by AP and OP in contrast to the M+ control (Fig. 7c).

Fig. 7
figure 7

Set of graphics displaying recently assimilated C results within different fractions of the microbial populations. (a) Box-plot showing the freshly assimilated C (μg) into AMF PLFA biomarkers within the fungal compartment for the different P sources (mean values ± standard error, n = 3). (b) Box-plot showing the freshly assimilated C (μg) into bacterial PLFA biomarkers within the fungal compartment for the different phosphorus sources (mean values ± standard error, n = 3). On the right side, the B+ stands for Gram-positive PLFA bacterial biomarker, and on the right side, B− for Gram-negative bacteria. (c) Box-plot showing the freshly assimilated C (μg) into AMF NLFA biomarkers in the fungal compartments for the different P sources (mean values ± standard error, n = 3). Within each P source on top of each box-plot significant differences between the content of freshly assimilated C (μg) into PLFA or NLFA biomarkers are shown with different small letters, as result of a one-way ANOVA (p < 0.05). Treatments with significant differences between the percentages of freshly assimilated C into PLFA or NLFA biomarkers are indicated by different capital letters (p < 0.05) as analyzed by one-way ANOVA. Abbreviations are OP, orthophosphate; AP, apatite; PA, phytic acid; M+, mycorrhizal plant with no P

Discussion

We tested the ability of R. irregularis taking up P from three different sources of different availability. To that end, we performed two consecutive experiments; in experiment 1, we investigated whether a C for P trading by the AM plants exists at mobilization of P derived from sources with different availability. In experiment 2, we explored the fate of freshly assimilated C derived to nourish the AMF mobilizing the different P sources. We decided on the 84th day of the sampling, because AM plants exhibited the larger P incorporation and not exhibited any significant differences in the amount of P incorporated from each source.

Experiment 1

Phosphorus uptake from different accessible sources by the arbuscular mycorrhiza symbiosis

The P sources were exclusively mobilized by the AMF and incorporated in different amounts into the plant tissues (Fig. 2). Additionally, the results of higher shoot to root biomass (Fig. S1) in those treatments mobilizing a P source are consistent with those ones previously discussed by Veresoglou et al. (2012), since fungal symbionts are more efficient scavengers for soil nutrients than plant roots; thus, root dry weight allocation would be lower with mycorrhizal fungi, as they would substitute for root uptake. Hence, it is expected that alleviation of host nutrient limitation due to inoculation by AMF may decrease plant root-to-shoot ratio, compared with non-mycorrhizal plants due to improved plant nutrient status (Holste et al. 2016). The AM fungal extraradical mycelium can directly acquire phosphate ions in the form of OP beyond the P depletion zone (Hodge 2017). Thus, our results demonstrated the suitability of the double-membrane system which prevented the influence of the roots on the P uptake, and therefore proved the importance of R. irregularis taking up P derived from the different sources.

The PA needs to be hydrolyzed by phytases of plant or microbial origin for its mineralization and phosphate release (Baldwin et al. 2001). R. irregularis DAOM 197198 is able to secrete a type of acid phosphatase, which contributes to the PA mineralization (Tisserant et al. 2012). Several studies have shown the ability of Rhizophagus species to hydrolyze organic P and transfer it through the mycelium and making a significant contribution to plant P uptake from sources such as lecithin, RNA, and PA (Koide and Kabir 2000; Sato et al. 2015). Utilization of organic P is thus assumed to contribute to AM plant P nutrition as inorganic P. Likewise, we did not observe significant differences between the amounts of P mobilized from PA and OP during most of the time of the experiment.

We observed that the AM plant mobilizing the AP clearly incorporated more P than was soluble in water, a fact that points out to an active biological weathering. It is widely accepted that AM plants can access the P contained in the AP by joint action of roots and hyphae (Murdoch et al. 1967). Souchie et al. (2010) observed a greater clover growth rates in those treatments mycorrhized with AMF and fed with AP, as the solely P source in comparison with the non-mycorrhized treatments. The AM hyphae appear to enter AP mineral structures, while plant roots barely can take up P from primary minerals as they are too unreactive (Koele et al. 2014). Burghelea et al. (2015) observed in a similar approach as ours, that over 124 days, AM mycorrhized plant growth was enhanced in presence of rhyolite rocks, due to an efficient P mining. Different authors have demonstrated the ability of AMF to mobilize P from AP, e.g., Rhizophagus fasciculatus and Rhizophagus tenuis (Powell and Daniel 1978), Rhizophagus manihotis (Ness and Vlek 2000), Rhizophagus clarum (Alloush and Clark 2001), and Gigaspora margarita (Ramirez et al. 2009), and Koele et al. (2014) found mineral weathering “tunnels” under both arbuscular and ectomycorrhizal associations coupled to P uptake from AP. They pointed out to the rhizosphere acidification as one likely mechanism for the AP weathering by soil mycorrhizal fungi, saprotrophic fungi, and bacteria. Two recent papers have also investigated the abilities of R. irregularis in phosphate mobilization from AP (Taktek et al. 2017; Pel et al. 2018). Both publications showed the AMF utilizing poorly soluble AP to deliver phosphate to host plants. Compared with the study of Taktek et al. (2017), we used a complete plant and not a transformed root culture. We also found another significant difference with the investigation of Pel et al. (2018). Contrastingly to our experimental design, they did not spatially separate the AP from the roots. Thus, our mesocosms offered an advantageous design, where exclusively the hyphae of R. irregularis were able to mobilize the phosphate from OP, PA, and the AP.

Metabolic activity and development of R. irregularis under variable P sources

We used the respired CO2-C to OC ratio, as a proxy for the metabolic activity in the fungal compartment. The mesocosm design guaranteed that all C measured in the fungal compartment was exclusively carried by the AMF. The sources of respired CO2 in the fungal compartment include R. irregularis autotrophic respiration, plus the heterotrophic one derived from the associated microbiota and dead hyphae/bacteria. The CO2-C to OC ratio was significantly higher for treatments containing PA and AP than OP until day 84 (Fig. 3), indicating a higher metabolic activity of AMF, in order to mobilize P from the least available sources. Thus, in our one-one system, the AMF seems to adjust its energy requirements precisely to the accessibility of the P sources. These results correspond to those already obtained in our previous study, where the less accessible P sources associated to iron oxides exhibited the highest metabolic activities (Andrino et al. 2019). Hence, it can be inferred that treatments containing less accessible P sources exhibited higher C investments in autotrophic and heterotrophic metabolic processes during P incorporation in the AM plant.

In addition to the metabolic activity, we used the ratios PLFA and NLFA 16:1ω5c to the total plant P uptake as indicators of energy investment into infrastructure and energy storage per P incorporated, respectively (Fig. 4a and b). The PLFA 16:1ω5c to P and NLFA 16:1ω5c to P ratios showed higher values in those treatments mobilizing P from PA and AP, compared with OP. One likely justification to increase the fungal infrastructure in the presence of P forms like PA and AP is the need to develop more extraradical hyphae to mobilize and transport P more efficiently to the host plant, as AMF tend to develop more extensively under low-nutrient conditions (Bryla and Eissenstat 2005). At low P availability, Raven (2018) hypothesized a greater C investment on AMF mycelium would be needed to support an increased construction and maintenance costs of the mycelium, as is likely the case in the PA and AP treatments, which need to be mineralized or weathered, respectively, before P uptake. Jansa et al. (2011) hypothesized on the existence of different mechanisms for P mobilization by AMF: scavenging of OP, solubilizing AP, and hydrolyzing PA. They assumed different turnover rates for AMF being associated to the different mobilization strategies. According to Jansa et al. (2011), long-lived hyphae represent an advantage in mobilizing hardly accessible P sources like AP or PA, once approached. Contrasting to this, they hypothesized short-lived hyphae in case of OP exploitation in soil, as here a quick exploration of larger soil volumes is key, and the process of scavenging OP from soil solution itself does not require comparable amounts of energy. Hence, our results may support the hypothesis of Jansa et al. (2011), meaning that even when the P source is reached by the AMF, it still is growing to assist the mobilization of less accessible sources, on which mineralization and/or weathering processes will take place. Finally, the NLFA 16:1ω5c to PLFA 16:1ω5c ratio (Fig. 4c), an indicator of the preferential C allocation into lipid storage (Bååth 2003), suggests that AM plants mobilizing OP and AP required less energy storage, compared with those plants mobilizing PA at day 84. The AM plants mobilizing PA exhibited a larger investment into AMF mycelium and metabolism in contrast to the ones mobilizing AP that showed a smaller investment into the AMF infrastructure but significantly higher metabolism. Our experiment revealed different P acquisition strategies that resulted in different C costs for the AM plant. This is in accordance with the hypothesis proposed by Turner (2008), in which the acquisition of the different P compounds are classified by the energy investment that must be made to access the phosphate ions. Thus, under our experimental conditions, where AM plants took up P exclusively via the mycorrhizal pathway, we can deduce different trading costs of C for P, depending on the P source.

Experiment 2

To evaluate the current C-P trading costs made by the AM plants mobilizing the different P sources, another set of 84-day-old plants were labeled with 13CO2, since no significant differences were observed during experiment 1 regarding the amount of P incorporated at day 84. The selected time point offered us a unique opportunity to compare whether the different acquisition strategies described in experiment 1 entailed different energy investments to mobilize the P by tracing the pathways of the recent plant assimilates along the trophic chain of AMF and adjacent microbiota.

Photosynthetic rates and leaf areas

We measured the photosynthetic activity of each AM plant 1 day before the 13CO2 pulse labeling. All AM plants mobilizing a P source showed significantly higher photosynthetic rates than the M+ control. Additionally, the AM plants mobilizing OP exhibited the largest photosynthetic rates. As for the photosynthesis rates, also the foliar areas were greater in the AM plants mobilizing a P source, and plants mobilizing OP showed the greatest foliar areas (Fig. 6). At least three other studies have reported similar results to ours (Parádi et al. 2003; Wu and Xia 2006; Birhane et al. 2012), where AM plants with bigger leaf areas also showed a higher photosynthetic CO2 fixation. They attributed to a larger total P content in plant tissues as the main cause for the enhanced photosynthetic function, coupled to an enhanced C fixation derived into the AMF, in comparison with non-AM plants. Thus, our results point towards AM plants mobilizing P from the different sources and increased their photosynthetic activity and leaf area sizes, to keep a larger photosynthate transfer into the AMF.

Freshly assimilated C measured in the respired CO2 and the plant tissues

We used the respired CO2 at the fungal compartment as a proxy for the current metabolic activity in the fungal compartment, as AMF receive the recently fixed photosynthates from their host plants, using it for their growth, respiration, and biological maintenance (Jansa et al. 2011). In nutrient-deficient soils, the AMF extraradical mycelium can account as a major source of CO2 efflux, thus becoming an important pathway of C flux from host plants to the atmosphere (Tomè et al. 2016). We focused our efforts on accurately registering the first respiration hours in the fungal compartment, as Grimoldi et al. (2006), Moyano et al. (2007), and Bahn et al. (2009) demonstrated that AM mycelia may provide a rapid and important pathway of C flux from plants to the soil and atmosphere, providing evidence of direct respiratory losses by AMF. Johnson et al. (2002) also highlighted the value of thorough surveys to track short-term C transfer dynamics of AM plants to soil, as many studies perform pulse labeling trials with relatively long fixation periods or have collected samples many hours after the end of labeling and consequently may have overlooked the maximum C yields from roots to AMF. Unlike experiment 1, in experiment 2, the short observation time implies that only autotrophic respiration was determined at the fungal compartment. However, as we observed in experiment 1 (Fig. 3), the results of experiment 2 also exhibited higher metabolic rates in those AM plants mobilizing PA and AP than taking up OP (Fig. 5). In line with Bahn et al. (2009), we detected that the new photoassimilates were transported and respired belowground within the first 3 h after the pulse labeling started, for the AM plants mobilizing PA. And similar to our results, Johnson et al. (2002) showed that the release of 13CO2 from AMF peaked 9–14 h after labeling and during the first 21 h. Most of the C lost by the plants was respired by the AMF and mostly declined after the first 24 h. At the end of the respiration monitoring in the fungal compartment, AM plants mobilizing P from the PA showed the significantly largest cumulative 13CO2 (Fig. 6, pointing to a greater C investment to mobilize as for the other treatments.

The 13C contents in the leaves, shoots, roots, and substrates from plant and fungal compartments were significantly larger for treatments mobilizing OP, PA, and AP than those for the M+ treatment. This fits to the report of Johnson (2010) that AM plants might partition more C belowground under P-limiting conditions to develop the capacity for soil P extraction. The model of Johnson (2010) predicts a mutualistic outcome of AM symbioses, depending on the stoichiometry of available N and P. There will be mutualistic benefits at no N limitation and low P availability because a non-limited N supply tends to increase the photosynthetic capacity of the host plant. In our experimental design, the N was not limited since it was applied in the nutrient solution weekly. Our results corroborate Johnson’s postulates, since all AM plants that mobilized and incorporated a P source exhibited larger photosynthetic capacity, foliar areas, and 13C contents in all the plant tissue compartments compared with that of the M+ treatment (Fig. 6), to supply more C to the AMF for mining the P sources of lower availability.

Freshly assimilated C incorporated into different microbial communities

We traced the fate of the freshly assimilated C into the microbial communities of the fungal compartments by fatty acid biomarkers (Fig. 7. Our results revealed that treatments with P application frequently showed the largest 13C content in the AMF hyphae along with the significantly lowest 13C values in the bacterial biomarkers. Only a fraction was delivered to AMF accompanying microbiota, since we inoculated the tomato plants with the strain of R. irregularis DOAM 197198 not grown in a monoxenic culture, thus carrying the microorganisms naturally associated with its hyphosphere. Zhang et al. (2016) pointed out that the AMF interactions with specific bacteria depend upon background P availability. The AMF may act as a rapid hub for recent photosynthates providing important niches to hyphae-associated soil bacterial communities which are generally composed of specific assemblages of species that differ from those in the bulk soil (Kaiser et al. 2015). The hyphae of AMF may provide an increased area for interactions with other soil microorganisms, especially bacteria, which may in turn synergistically interact with AMF and thereby promote plant growth (Taktek et al. 2015). Among the most effective bacterial phosphate solubilizers are species of the B+ genera Streptomyces, Kocuria, Arthrobacter, Nocardiodes, Microbacterium, Bacillus, and the B− genera Pseudomonas, Enterobacter, Burkholderia, Serratia, Citrobacter, Xanthomonas, Rhizobium, Azospirillum, and Klebsiella (Battini et al. 2016; Osorio et al. 2017). The mechanisms to access the more stable P forms include phosphatase synthesis (e.g., acid phosphatase, phytase), soil pH lowering, and/or ligand exchange reactions mediated by low molecular weight organic acids (Osorio et al. 2017). Although not significantly different from the M+ control, treatments with PA and AP in their fungal compartments displayed the largest absolute 13C contents in B− bacterial biomarkers 27 h after 13CO2 labeling (Fig. 7b). The results show AMF altering the diversity and structure of bacterial communities in the fungal compartment and fueling P weathering (AP) or hydrolyzation (PA). In this regard, Kim et al. (1998) and Taktek et al. (2015, 2017) suggested a synergistic interaction between the hyphobacteria able to solubilize insoluble phosphate and an AMF to mobilize P from AP. A similar reasoning was made by Selvakumar et al. (2016), Lecomte et al. (2011), and Battini et al. (2016), who showed a synergistic interaction between the AMF and some bacteria with abilities to mineralize the PA. We estimated the cost of establishing an AM by means of the NLFA 16:1ω5c biomarker (Bååth 2003), since the AM pathway seems to respond quickly to changing soil nutrient by adapting the amount of plant C channeled through the fungus (Kaiser et al. 2015). The NLFA 16:1ω5c is stored in intraradical vesicles, spores, extraradical mycelium, and metabolized in the mycelium through the glyoxalate cycle, providing the major fungal energy source as respiratory substrate (Olsson and Johnson 2005). The freshly assimilated C incorporated into AMF NLFA biomarkers clearly indicated that PA was the P source with the most expensive acquisition cost, followed by AP and OP in contrast to the M+ control (Fig. 7c). In summary, AM plants with access to PA and AP diversified the fate of recently assimilated C, incorporating it into the PLFA and NLFA 16:1ω5c. The more stable and therefore less available P sources PA and AP received greater amounts of photosynthates that were invested in further growth (PLFA 16:1ω5c) and energy accumulation (NLFA 16:1ω5c), along with a potential contribution of B− bacteria in the mobilization of phosphate from both P sources.

Conclusions

In this study, we showed that AM plants took up P derived from orthophosphate (OP), apatite (AP), and phytic acid (PA) exclusively via the mycorrhizal pathway with different C-P trading costs. We identified different P acquisition strategies to mobilize the less available P sources PA and AP. In the fungal compartment, both sources exhibited the largest investments into fungal infrastructure (PLFA) and the largest metabolic rates (CO2-C/OC). Our results suggest that the less available P sources require more long-living mycelium infrastructure with higher metabolic rates, in contrast to the OP with a faster hyphal turnover and lower requirements of freshly assimilated C. We also observed that B− bacteria may contribute to P acquisition from PA and AP, suggesting a synergistic cooperation occur between both organisms. The C investments into P mobilization from P sources with differing accessibilities suggests the existence of a nexus between AMF P mining strategies and the amounts of C accumulated in terrestrial soils.

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Acknowledgments

The authors would like to thank Dr. Axel Don and Dr. Reinhard Well for opening the doors of the Thünen Institute for developing part of the experiment. The authors are also thankful for the great help and guidance received by Dr. Norman Gentsch, Silke Bokeloh, Elke Eichmann-Prusch, Anne Katrin Herwig, Ulrike Pieper, Heike Steffen, and Michael Klatt.

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Raw and derived data supporting the findings of this study are available from the corresponding author Alberto Andrino on request.

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Open Access funding provided by Projekt DEAL. Funding was provided within the Research Training Group 1798 “Plant-Soil-Interfaces” (PSI) of the Deutsche Forschungsgemeinschaft (DFG).

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Jens Boy, Georg Guggenberger, and Alberto Andrino designed the experiment. Alberto Andrino prepared the plant and fungal material. Stefan Burkart and Alberto Andrino carried out the Picarro measurements at the Institute of Climate Smart Agriculture facilities. Alberto Andrino conducted the experiment and analyzed the data. Jens Boy, Leopold Sauheitl, and Georg Guggenberger supervised the research. Alberto Andrino wrote the paper with contributions from Jens Boy, Georg Guggenberger, Leopold Sauheitl, and Stefan Burkart. All authors read and approved the final manuscript.

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Correspondence to Alberto Andrino.

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Andrino, A., Guggenberger, G., Sauheitl, L. et al. Carbon investment into mobilization of mineral and organic phosphorus by arbuscular mycorrhiza. Biol Fertil Soils 57, 47–64 (2021). https://doi.org/10.1007/s00374-020-01505-5

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Keywords

  • Apatite
  • Organic phosphorus
  • Phytate
  • Inorganic phosphorus
  • Arbuscular mycorrhiza
  • PLFA 16:1ω5c
  • NLFA 16:1ω5c
  • Photosynthesis
  • Stable carbon isotopes