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Endophytic bacteria from selenium-supplemented wheat plants could be useful for plant-growth promotion, biofortification and Gaeumannomyces graminis biocontrol in wheat production

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Abstract

In this study, we isolated putative plant-growth-promoting endophytic bacteria from selenium-supplemented wheat grown under field conditions. These bacterial strains belonged to Bacillus, Paenibacillus, Klebsiella, and Acinetobacter genera and showed genetic similarly with rhizospheric bacteria isolated in the same Andisol soil and with other endophytic strains previously reported. Strains isolated from selenium-supplemented wheat were highly tolerant to elevated selenium concentration (ranged from 60 to 180 mM), and showed potential plant-growth-promoting capabilities (auxin and siderophore production, phytate mineralization, and tricalcium phosphate solubilization). In addition, some strains like Acinetobacter sp. (strain E6.2), Bacillus sp. (strain E8.1), Bacillus sp., and Klebsiella sp. (strains E5 and E1) inhibited the growth of Gaeumannomyces graminis mycelia in vitro at 100, 50, and 30 %, respectively. These endophytic microorganisms would be useful for dual purposes: selenium biofortification of wheat plants and control of G. graminis, the principal soil-borne pathogen in volcanic soils from southern Chile.

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Acknowledgments

This study was supported by the Comisión Nacional de Investigación Científica y Tecnológica (CONICYT) postdoctoral scholarship N° 3130542 and CONICYT regular project N° 1100625 by the Chilean Government.

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Authors and Affiliations

  1. Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Avenida Francisco Salazar, 01145, Temuco, Araucanía, Chile

    Paola Durán, Jacquelinne J. Acuña, Milko A. Jorquera, Cecilia Paredes & María de la Luz Mora

  2. Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín (CSIC), Profesor Albareda n° 1, 1808, Granada, Spain

    Rosario Azcón

  3. Soil Science and Plant Nutrition, School of Earth and Environment, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia

    Zed Rengel

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  1. Paola Durán
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  2. Jacquelinne J. Acuña
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  3. Milko A. Jorquera
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  7. María de la Luz Mora
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Correspondence to María de la Luz Mora.

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Durán, P., Acuña, J.J., Jorquera, M.A. et al. Endophytic bacteria from selenium-supplemented wheat plants could be useful for plant-growth promotion, biofortification and Gaeumannomyces graminis biocontrol in wheat production. Biol Fertil Soils 50, 983–990 (2014). https://doi.org/10.1007/s00374-014-0920-0

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  • DOI: https://doi.org/10.1007/s00374-014-0920-0

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