Abstract
An automated laboratory soil incubation system enabled the effects on gaseous emissions from a soil to be quantified accurately, when amended with slurry plus a nitrification inhibitor: dicyandiamide (DCD), or 3,4-dimethylpyrazole phosphate (DMPP). Nitrification inhibitors applied with slurry under simulated Portuguese conditions were very efficient in reducing N2O emission, and did not increase CH4 emissions significantly, when the soil was predominantly aerobic. The inhibitors were also indirectly effective in reducing N2O emissions due to denitrification during a subsequent anaerobic phase. All gaseous emissions followed strong diurnal patterns that were positively correlated with soil temperature and obeyed a Q10=2 relationship. The widespread use of DCD and DMPP inhibitors with slurry applied to Portuguese soils could have the potential to reduce N2O emissions from this source by ten- to 20-fold.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bol R, Toyoda S, Yamulki S, Hawkins JMB, Cardenas LM, Yoshida N (2003) Dual isotope and isotopomer ratios of N2O emitted from a temperate grassland soil after fertiliser application. Rapid Commun Mass Spectrom 17:2550–2556
Bouwman AF, Fung I, Matthews E, John J (1993) Global analysis of the potential for N2O production in natural soils. Global Biogeochem Cycles 7:557–597
Cardenas LM, Hawkins JMB, Chadwick D, Scholefield D (2003) Biogenic gas emissions from soils measured using a new automated laboratory system. Soil Biol Biochem 35:867–870
Chadwick D, Pain BF, Brookman SKE (2000) Nitrous oxide emissions following application of animal manures to grassland. J Environ Qual 29:277–287
Christensen S (1983) Nitrous oxide emission from soil under permanent grass: seasonal and diurnal fluctuations as influenced by manuring and fertilization. Soil Biol Biochem 15:531–536
Darrah PR, Nye PH, White RE (1985) Modelling growth responses of soil nitrifiers to additions of ammonium sulphate and ammonium chloride. Plant Soil 86:425–39
Dittert K, Bol R, King R, Chadwick D, Hatch D (2001) Use of a novel nitrification inhibitor to reduce nitrous oxide emission from 15N-labelled dairy slurry injected into soil. Rapid Commun Mass Spectrom 15:1291–1296
Erickson HE, Keller M (1997) Tropical land use change and soil emissions of nitrogen oxides. Soil Use Manage 13:278–287
Fettweis U, Mittelstaedt W, Schimansky C, Fuhr F (2001) Lysimeter studies on the translocation of the 14C-labelled nitrification inhibitor DMPP in a gleyic cambisol. Biol Fertil Soils 34:126–130
Hobbs PJ, Johnson R, Chadwick D (1999) A novel technique to determine organic processes in pig wastes. J Sci Food Agric 79:199–205
IPCC (Intergovernmental Panel on Climate Change) (1997) Greenhouse gas emissions from agricultural soils. In: Houghton JT, et al. (eds) Greenhouse gas inventory reference manual. Revised 1996 IPCC guidelines for national greenhouse gas inventories. IPCC, OECD, IES, UK Meteorological Office, Bracknell, Hertfordshire
Jarvis SC, Hatch DJ, Pain BF, Klarenbeek JV (1994) Denitrification and the evolution of nitrous oxide after the application of cattle slurry to a peat soil. Plant Soil 166:231–241
Kamphake LJ, Hannah SA, Cohen JM (1967) Automated analysis for nitrate by hydrazine reduction. Water Res 1:205–216
Macadam XMB, del Prado A, Merino P, Estavillo JM, Pinto M, Gonzales-Murua C (2003) Dicyandiamide and 3,4-dimethyl pyrazole phosphate decrease N2O emissions from grassland but dicyandiamide produces deleterious effects on clover. J Plant Physiol 160:1517–1523
Majumdar D, Pathak H, Kumar S, Jain MC (2002) Nitrous oxide emission from a sandy loam Incepticol under irrigated wheat in India as influenced by different nitrification inhibitors. Agric Ecosyst Environ 91:283–293
Merino P, Estavillo JM, Besga G, Pinto M, González-Mura C (2001) Nitrification and denitrification derived N2O production from grassland soil under application of DCD and Actilith F2. Nutr Cycl Agroecosyst 60:9–14
Merino P, Estavillo JM, Graciolli LA, Pinto M, Lacuesta M, Munoz-Rueda A, Gonzalez-Murua C (2002) Mitigation of N2O emissions from grassland by nitrification inhibitor and Actilith F2 applied with fertilizer and cattle slurry. Soil Use Manage 18:135–141
Oenema O, Velthof GL, Yamulki S, Jarvis SC (1997) Nitrous oxide emissions from grassland. Soil Use Manage 13:288–295
Pathak H, Nedwell DB (2001) Nitrous oxide emission from soil with different fertilizers, water levels and nitrification inhibitors. Water Air Soil Pollut 129:217–228
Prosser IL (1989) Autotrophic nitrification in bacteria. Adv Microb Physiol 30:125–181
Sánchez L, Díez JA, Vallejo A, Cartagena MC (2001) Denitrification losses from irrigated crops in central Spain. Soil Biol Biochem 33:1201–1209
Schmidt EL (1982) Nitrification in soil. In: Nitrogen in agricultural soils. Agronomy Monograph No 22. Am Soc Agron, Madison, WI, pp 253–288
Scholefield D, Hawkins JMB, Jackson SM (1997) Development of a helium soil incubation technique for direct measurement of nitrous oxide and dinitrogen fluxes during denitrification. Soil Biol Biochem 29:1345–1352
Searle PL (1984) The Berhlot or indophenol reaction and its use in the analysis chemistry of nitrogen. Analyst 109:549–565
Smith KA, McTaggart IP, Tsuruta H (1997) Emissions of N2O and NO associated with nitrogen fertilization in intensive agriculture, and the potential for mitigation. Soil Use Manage 13:296–304
Teske W, Matzel W (1988) Die Beeinflussung der nitrifikations-hemmenden Wirkung von Dicyandiamid durch Abbau und Verlagerung im Boden. Arch Acker Pflanzenbau Bodenkd 32:241–246
Tlustos P, Willison TW, Baker JC, Murphy DV, Pavlikova D, Goulding KWT, Powlson DS (1999) Short-term effects of nitrogen on methane oxidation in soils. Biol Fertil Soils 28:64–70
Trindade H, Coutinho J, van Beusichem ML, Scholefield D, Moreira N (1997) Nitrate leaching from sandy loam soils under a double-cropping forage system estimated from suction-probe measurements. Plant Soil 195:247–256
Vallejo A, Diez JA, Lopez-Valdivia LM, Gasco A, Jiménez C (2001) Nitrous oxide emission and denitrification nitrogen losses from soils treated with isobutylenediurea and urea plus dicyandiamide. Biol Fertil Soils 34:248–257
Weier KL, Doran JW, Power JF, Walters DT (1993) Denitrification and the dinitrogen/nitrous oxide ratio as affected by soil water, available carbon and nitrate. Soil Sci Soc Am J 57:66–72
Weiske A, Benckiser G, Herbert T, Ottow JCG (2001) Influence of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) in comparison to dicyandiamide (DCD) on nitrous oxide emissions, carbon dioxide fluxes and methane oxidation during 3 years of repeated application in field experiments. Biol Fertil Soils 34:109–117
Xu X, Boeckx P, van Cleepput O, Zhou L (2002) Urease and nitrification inhibitors to reduce emissions of CH4 and N2O in rice production. Nutr Cycl Agroecosyst 64:203–211
Yamulki S, Toyoda S, Yoshida N, Veldkamp E, Grant B, Bol R (2001) Diurnal fluxes and the isotopomer ratios of N2O in a temperate grassland following urine amendments. Rapid Commun Mass Spectrom 15:1263–1269
Zerulla W, Barth T, Dressel J, Erhardt K, von Locquenghein KH, Pasda G, Radle M, Wissemeier H (2001) 3,4-Dimethylpyrazole phosphate (DMPP)—a new nitrification inhibitor for agriculture and horticulture. Biol Fertil Soils 34:79–84
Acknowledgements
The authors would like to thank the following for their help in this work: A. Bristow, S. Brookman, E. Dixon and P. Hobbs. The DMPP and DCD inhibitors were provided by COMPO (Spain)/BASF (Germany) and Adubos de Portugal, respectively. We are grateful to the British Council (Treaty of Windsor Programme) and Conselho de Reitores das Universidades Portuguesas for providing additional funding. IGER is supported by the Biotechnology and Biological Sciences Research Council.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hatch, D., Trindade, H., Cardenas, L. et al. Laboratory study of the effects of two nitrification inhibitors on greenhouse gas emissions from a slurry-treated arable soil: impact of diurnal temperature cycle. Biol Fertil Soils 41, 225–232 (2005). https://doi.org/10.1007/s00374-005-0836-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00374-005-0836-9