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Early effects of tillage and crop rotation on arbuscular mycorrhizal fungal propagules in an Ultisol

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Abstract

The aim of this work was to study the early influence of conventional tillage (CT) and no-tillage (NT) on arbuscular mycorrhizal fungal (AMF) propagules. A short 2-year-course crop rotation, i.e. trial consisting of a succession of wheat and oat, was studied in a typic Chilean Ultisol from the second to fourth year after the beginning of the experiment. Measurements included mycorrhizal characteristics and some soil properties in order to explain their influence on AMF propagules. Soil samples were taken yearly in autumn (fallow period) and in early spring (flowering). Significant differences in AMF hyphal length were observed between NT and CT in the first year, but such differences disappeared thereafter. No differences in metabolically active hyphae were obtained with wheat or oat under the two tillage systems. Mycorrhizal root colonization was always higher under NT than under CT. The number of AMF spores was also higher under NT than under CT, ranging from 158 to 641 spores per 100 cm3. Twenty-two AMF species including eight Glomus spp., six Acaulospora spp., four Scutellospora spp., one Archaeospora sp., one Diversispora sp., one Entrophospora sp. and one Pacispora sp. were observed in both agro-ecosystems. Higher spore number of Acaulospora spp. was found under wheat than under oat and under CT than under NT, whilst more spores of Scutellospora spp. were observed under NT than under CT. From all mycorrhizal characteristics, spore number could be visualized as an early and useful indicator of the effect of tillage systems on mycorrhizal propagules in short-term experiments.

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Acknowledgements

This research was supported by Grants C-13755-28 and Fondecyt 1020306 and 7040062 given by Fundación Andes and Comisión Nacional Científica y Tecnológica, respectively. The authors also thank Dr. Ewald Sieverding for his helpful suggestions related to the manuscript.

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  1. Universidad de La Frontera, Casilla 54-D, Temuco, Chile

    C. G. Castillo, R. Rubio, J. L. Rouanet & F. Borie

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  1. C. G. Castillo
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  3. J. L. Rouanet
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Correspondence to F. Borie.

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Castillo, C.G., Rubio, R., Rouanet, J.L. et al. Early effects of tillage and crop rotation on arbuscular mycorrhizal fungal propagules in an Ultisol. Biol Fertil Soils 43, 83–92 (2006). https://doi.org/10.1007/s00374-005-0067-0

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