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Effects of using different host plants and long-term fertilization systems on population sizes of infective arbuscular mycorrhizal fungi

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Abstract

The effect of cultivation of mycorrhizal and non-mycorrhizal plants and mineral fertilization on the arbuscular mycorrhizal fungal (AMF) community structure of maize (Zea mays L.) plants was studied. Soil samples were collected from two field experiments treated for 5 years with three fertilization systems (Control – no fertilization; Mineral – NPK fertilization; and Organic – Farmyard manure fertilization). Soil samples containing soil and root fragments of rapeseed (Brassica napus L., non-mycorrhizal plant) and wheat (Triticum aestivum L., mycorrhizal plant) collected from the field plots were used as native microbial inoculum sources to maize plants. Maize plants were sown in pots containing these inoculum sources for four months under glasshouse conditions. Colonization of wheat roots by AMF, AMF community structure, AMF diversity (Shannon’s index), AMF dominance (Simpson’s index) and growth of maize were investigated. Sixteen AMF species were identified from rhizosphere soil samples as different species of genera Acaulospora, Claroideoglomus, Dentiscutata, Funneliformis, Gigaspora, Quatunica, Racocetra, and Rhizoglomus. Maize plants grown in manure-fertilized soils had a distinct AMF community structure from plants either fertilized with mineral NPK-fertilizer or non-fertilized. The results also showed that inoculum from non-mycorrhizal plants combined with mineral fertilization decreased AMF diversity (Shannon’s index), AMF dominance (Simpson’s index) and growth of maize. Our findings suggest that non-mycorrhizal plants, such as B. napus, can negatively affect the presence and the effects of soil inoculation on maize growth. Also, our results highlight the importance of considering the long-term effect of rapeseed cultivation system on the reduction of population sizes of infective AMF, and its effect on succeeding annual crops.

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Acknowledgements

Special thanks to Joana Costa for the valuable discussions and checking of English grammar. The authors also thank the two anonymous reviewers for the helpful comments, which greatly improved a previous version of the manuscript.

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  1. Department of Soils and Rural Engineering, Agrarian Sciences Centre, Federal University of Paraiba, Areia, Paraiba, 58397-000, Brazil

    Tancredo Augusto Feitosa de Souza & Djail Santos

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de Souza, T.A.F., Santos, D. Effects of using different host plants and long-term fertilization systems on population sizes of infective arbuscular mycorrhizal fungi. Symbiosis 76, 139–149 (2018). https://doi.org/10.1007/s13199-018-0546-3

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