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Does Japanese medaka (Oryzias latipes) exhibit a gill Na+/K+-ATPase isoform switch during salinity change?

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Abstract

Some euryhaline teleosts exhibit a switch in gill Na+/K+-ATPase (Nka) α isoform when moving between fresh water (FW) and seawater (SW). The present study tested the hypothesis that a similar mechanism is present in Japanese medaka and whether salinity affects ouabain, Mg2+, Na+ and K+ affinity of the gill enzyme. Phylogenetic analysis classified six separate medaka Nka α isoforms (α1a, α1b, α1c, α2, α3a and α3b). Medaka acclimated long-term (>30 days) to either FW or SW had similar gill expression of α1c, α2, α3a and α3b, while both α1a and α1b were elevated in SW. Since a potential isoform shift may rely on early changes in transcript abundance, we conducted two short-term (1–3 days) salinity transfer experiments. FW to SW acclimation induced an elevation of α1b and α1a after 1 and 3 days. SW to FW acclimation reduced α1b after 3 days with no other α isoforms affected. To verify that the responses were typical, additional transport proteins were examined. Gill ncc and nhe3 expression were elevated in FW, while cftr and nkcc1a were up-regulated in SW. This is in accordance with putative roles in ion-uptake and secretion. SW-acclimated medaka had higher gill Nka V max and lower apparent K m for Na+ compared to FW fish, while apparent affinities for K+, Mg2+ and ouabain were unchanged. The present study showed that the Japanese medaka does not exhibit a salinity-induced α isoform switch and therefore suggests that Na+ affinity changes involve altered posttranslational modification or intermolecular interactions.

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Acknowledgments

This work was supported by a grant from the National Science Foundation [IBN 12-51616] and the Arkansas Biosciences Institute to C. K. T. The Fulbright Commission supported S. S. M. with a visiting professor scholarship. The Cell and Molecular Biology program at the University of Arkansas supported R. J. B. with a research stipend. The authors would like to thank Ms. R. T. Trubitt for technical help.

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  1. Department of Biological Sciences, University of Arkansas, SCEN 601, Fayetteville, AR, 72701, USA

    Rebecca J. Bollinger, Steffen S. Madsen, Maryline C. Bossus & Christian K. Tipsmark

  2. Department of Biology, University of Southern Denmark, Campusvej 55, 5230, Odense M, Denmark

    Steffen S. Madsen

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  1. Rebecca J. Bollinger
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Correspondence to Christian K. Tipsmark.

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Communicated by I.D. Hume.

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Bollinger, R.J., Madsen, S.S., Bossus, M.C. et al. Does Japanese medaka (Oryzias latipes) exhibit a gill Na+/K+-ATPase isoform switch during salinity change?. J Comp Physiol B 186, 485–501 (2016). https://doi.org/10.1007/s00360-016-0972-6

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