Abstract
Here, we studied the life-long monogamous zebra finch, to examine the relationship between circulating sex steroid profiles and pair-maintenance behavior in pairs of wild-caught zebra finches (paired in the laboratory for >1 month). We used liquid chromatography–tandem mass spectrometry to examine a total of eight androgens and progestins [pregnenolone, progesterone, dehydroepiandrosterone (DHEA), androstenediol, pregnan-3,17-diol-20-one, androsterone, androstanediol, and testosterone]. In the plasma, only pregnenolone, progesterone, DHEA, and testosterone were above the limit of quantification. Sex steroid profiles were similar between males and females, with only circulating progesterone levels significantly different between the sexes (female > male). Circulating pregnenolone levels were high in both sexes, suggesting that pregnenolone might serve as a circulating prohormone for local steroid synthesis in zebra finches. Furthermore, circulating testosterone levels were extremely low in both sexes. Additionally, we found no correlations between circulating steroid levels and pair-maintenance behavior. Taken together, our data raise several interesting questions about the neuroendocrinology of zebra finches.
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Acknowledgments
This research was made possible by an impressive support staff at Fowlers Gap Semi-Arid Zone Research Station, University of New South Wales Australia. Specifically, thank you to: Keith Leggett, Garry Dowling, Mark Tilley and Vicki Dowling for logistical support at Fowlers Gap. For help with the aviary set up, thank you to Dr. Emilie Perez, Ingrid Boucaud, and Dr. Clementine Vignal. For help with R Statistics and statistical analyses, thank you to Dr. Laura Weir and Dr. Jeffery Joy. For comments on the manuscript, thank you to Dr. Benjamin Sandkam, Dr. Matthew Taves, and three anonymous reviewers. Funding sources included: Canadian Institutes of Health Research (CIHR) Operating Grant 133606 to KKS; National Science Foundation graduate research fellowship (NSF GRFP) to NHP; and Australian Research Council Grant DP130100417 to SCG and KLB.
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Prior, N.H., Yap, K.N., Adomat, H.H. et al. Sex steroid profiles and pair-maintenance behavior of captive wild-caught zebra finches (Taeniopygia guttata). J Comp Physiol A 202, 35–44 (2016). https://doi.org/10.1007/s00359-015-1050-3
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DOI: https://doi.org/10.1007/s00359-015-1050-3