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Development of longitudinal smooth muscle in the posterior mesenteric artery and purinergic regulation of its contractile responses in chickens

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Abstract

This study was designed to clarify development and the neural regulation of longitudinal smooth muscle in the chicken posterior mesenteric artery to generate new hypotheses for the roles of arterial longitudinal muscles. The existence of longitudinal muscles was examined with hematoxylin-eosin staining. A well-developed longitudinal muscle layer exists in the posterior mesenteric artery of adult female chickens but not adult male chickens. The muscle layer is poorly developed in chickens aged < 15 weeks, even in female chickens. Mechanical responses of muscles were recorded and perivascular nerves were stimulated by electrical field stimulation (EFS). EFS induced monophasic contractions in longitudinal muscle of the posterior mesenteric artery segment, and those responses were inhibited by pretreatment with tetrodotoxin. Blockers for cholinoceptors and adrenoceptors did not affect EFS-evoked contractions but an antagonist for P2X purinoceptors blocked them. The present study demonstrated that the longitudinal muscle in the posterior mesenteric artery of the domestic fowl develops between the 5th and 15th week of life, suggesting that its development is involved in oviposition. The longitudinal muscle might have a role in resisting extensional stress from the oviduct containing eggs. Moreover, the arterial longitudinal muscle is regulated by purinergic neurons via P2X purinoceptors.

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Abbreviations

EFS:

Electrical field stimulation

HE:

Hematoxylin and eosin

NANC:

Non-adrenergic non-cholinergic

NF279:

8,8′-[Carbonylbis(imino-4,1-phenylenecarbonylimino-4,1-phenylenecarbonylimino)]bis-1,3,5-naphthalenetrisulfonic acid hexasodium salt

RT-PCR:

Reverse transcription-polymerase chain reaction

SD:

Standard deviation

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Acknowledgments

This work was supported in part by Grants-in-Aid for Scientific Research (KAKENHI) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan [Research Project Number: 15580256, 16580238, 24580423]. All experiments were conducted according to the Gifu University guidelines for proper animal experiments.

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Authors and Affiliations

  1. Laboratory of Physiology, Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, Yanagido 1-1, Gifu, 501-1193, Japan

    Takahiko Shiina, Masato Koga, Tadashi Takewaki & Yasutake Shimizu

  2. Laboratory of Anatomy, Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, Yanagido 1-1, Gifu, 501-1193, Japan

    Shouichiro Saito & Yasuro Atoji

Authors
  1. Takahiko Shiina
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  2. Masato Koga
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  3. Shouichiro Saito
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  4. Yasuro Atoji
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  5. Tadashi Takewaki
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  6. Yasutake Shimizu
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Corresponding author

Correspondence to Yasutake Shimizu.

Additional information

T. Shiina and M. Koga contributed equally to this work.

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Shiina, T., Koga, M., Saito, S. et al. Development of longitudinal smooth muscle in the posterior mesenteric artery and purinergic regulation of its contractile responses in chickens. J Comp Physiol A 199, 857–865 (2013). https://doi.org/10.1007/s00359-013-0848-0

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  • DOI: https://doi.org/10.1007/s00359-013-0848-0

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