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Cochlear function in mice following inhalation of brevetoxin-3

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Abstract

Brevetoxin-3 was shown previously to adversely affect central auditory function in goldfish. The present study evaluated the effects of exposure to this agent on cochlear function in mice using the 2f1-f2 distortion-product otoacoustic emission (DPOAE). Towards this end, inbred CBA/CaJ mice were exposed to a relatively high concentration of brevetoxin-3 (~400 μg/m3) by nose-only inhalation for a 2-h period. Further, a subset of these mice received a second exposure a day later that lasted for an additional 4 h. Mice exposed only once for 2 h did not exhibit any notable cochlear effects. Similarly, mice exposed two times, for a cumulative dose of 6 h, exhibited essentially no change in DPOAE levels.

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Abbreviations

ABR:

Auditory brainstem response

DP-gram:

Distortion-product otoacoustic emission gram

DPOAE:

Distortion-product otoacoustic emission

DSP:

Digital signal processor

FFT:

Fast Fourier transform

GM:

Geometric mean

MMAD:

Mass median aerodynamic diameter

NF:

Noise floor

OHC:

Outer hair cell

SD:

Standard deviation

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Acknowledgements

This research was funded in part by grants from the National Institutes of Health (ES10594, DC00613, DC03114). The authors thank Amber Dison, Carolyn Elliott, Dolores Esparza, Dean Kracko, Colleen Santistevan, and Thomas Argubright for technical assistance. The experiment was conducted in compliance with federal laws of the United States of America and guidance concerning the use of animals in scientific research

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Authors and Affiliations

  1. Lovelace Respiratory Research Institute, Albuquerque, NM, USA

    Janet M. Benson, Sarah E. Durr, Andrea Gomez & Jacob McDonald

  2. Department of Otolaryngology, University of Colorado Health Sciences Center, Denver, CO, USA

    Barden B. Stagner, Glen K. Martin & Brenda L. Lonsbury-Martin

  3. NIEHS Marine and Freshwater Biomedical Sciences Center, University of Miami, Miami, FL, USA

    Melissa Friedman & Lora E. Fleming

  4. National Center for Environmental Health, Center for Disease Control and Prevention, Atlanta, GA, USA

    Lorraine C. Backer

  5. Center for Marine Science Research, University of North Carolina at Wilmington, Wilmington, NC, USA

    Daniel G. Baden, Andrea Bourdelais & Jerome Naar

  6. Research Service (151), Jerry Pettis Memorial Veterans Medical Center, 11201 Benton Street, Loma Linda, CA, 92357, USA

    Barden B. Stagner

Authors
  1. Janet M. Benson
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  2. Barden B. Stagner
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  3. Glen K. Martin
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  4. Melissa Friedman
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  5. Sarah E. Durr
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  6. Andrea Gomez
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  7. Jacob McDonald
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  8. Lora E. Fleming
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  9. Lorraine C. Backer
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  10. Daniel G. Baden
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  11. Andrea Bourdelais
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  12. Jerome Naar
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  13. Brenda L. Lonsbury-Martin
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Corresponding author

Correspondence to Glen K. Martin.

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Benson, J.M., Stagner, B.B., Martin, G.K. et al. Cochlear function in mice following inhalation of brevetoxin-3. J Comp Physiol A 191, 619–626 (2005). https://doi.org/10.1007/s00359-005-0613-0

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  • DOI: https://doi.org/10.1007/s00359-005-0613-0

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