Abstract
The ability to understand and visualize complex flow structures in microfluidic and biological systems relies heavily on the resolving power of three-dimensional (3D) particle velocimetry techniques. We propose a simple technique for acquiring volumetric particle information with the potential for microsecond time resolution. By utilizing a fast varifocal lens in a modified wide-field microscope, we capture both volumetric and planar information with microsecond time resolution. The technique is demonstrated by tracking particle motions in the complex, three-dimensional flow in a high Reynolds number laminar flow at a branching arrow-shaped junction.
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Acknowledgements
The authors thank TAG Optics Inc., David Amrhein, and Christian Therlault for technical support, and gratefully acknowledge financial support from the NSF (Grant No. CMMI-1235291) and the Ministry of Education, Republic of China. T-H. Chen thanks Marcus Hultmark and Romain Fardel for useful discussions.
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NSF (Grant No. CMMI-1235291) and the Ministry of Education, Republic of China.
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Chen, TH., Ault, J.T., Stone, H.A. et al. High-speed axial-scanning wide-field microscopy for volumetric particle tracking velocimetry. Exp Fluids 58, 41 (2017). https://doi.org/10.1007/s00348-017-2316-z
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DOI: https://doi.org/10.1007/s00348-017-2316-z