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Characterization of a Homeodomain-Leucine Zipper Gene 12: Gene Silencing in Pepper and Arabidopsis-Based Overexpression During Abiotic Stress

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Abstract

Homeodomain-Leucine Zipper (HD-Zip) proteins are important ubiquitous and diverse molecular chaperones in plants. We characterized a gene CaATHB-12 derived from HD-Zip I subfamily which was intensively induced by exogenous abscisic acid (ABA), salt, and mannitol applications in a pepper cultivar. Efficient gene silencing lines were created from pepper, and stable heterologous overexpression lines were created from Arabidopsis to achieve a comprehensive exploration of gene function. The functional study of CaATHB-12 in pepper increased plant sensitivity to ABA stress, while the over-expressing CaATHB-12 in Arabidopsis lines revealed that tolerance to ABA, salt, and mannitol stresses was decreased. Furthermore, CaATHB-12 plays a fundamental role in elevating the tolerance to these stresses through the increased expression of other stress related genes, increasing the activities of antioxidant enzymes and scavenging the reactive oxygen species. The studied functions of the CaATHB-12 gene may provide some insights in exquisite molecular detail by pursuing signal transduction mechanisms that converge on gene expression patterns.

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All data generated or analysed during this study are included in this manuscript and its supplementary information files.

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Acknowledgements

This work was supported through funding from the National Natural Science Foundation of China (No. 31772309, No. 31860556 and No. U1603102) and China Agriculture Research System of MOF and MARA (CARS-24-G-01).

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  1. College of Horticulture, Northwest A and F University, Yangling, 712100, Shaanxi, China

    Ruixing Zhang, Quanhui Li, Jingjing Xiao, Saeed ul Haq, Guoxin Cheng & Zhenhui Gong

  2. Department of Agricultural Sciences, The University of Haripur, Haripur, Pakistan

    Abid Khan

  3. College of Agriculture, Ningxia University, Yinchuan, 750002, Ningxia, China

    Guoxin Cheng

  4. Institute of Cash Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050051, Hebei, China

    Huiling Yan

Authors
  1. Ruixing Zhang
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  2. Quanhui Li
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  3. Jingjing Xiao
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  4. Saeed ul Haq
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  5. Abid Khan
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  6. Guoxin Cheng
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  7. Huiling Yan
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  8. Zhenhui Gong
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Contributions

RXZ and ZHG: Conceptualization, Methodology, Investigation, Data curation, Writing—original draft. RXZ, QHL, and GXC: Writing—review and editing. JJX: Conceptualization, Visualization, Investigation, Writing—review and editing. AK and SH: Methodology, Writing—review and editing. HLY and ZHG: Supervision, Visualization, Resources, Writing—review and editing. All authors have read and approved the final manuscript.

Corresponding authors

Correspondence to Huiling Yan or Zhenhui Gong.

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The authors declare that they have no conflicts of interest in this work. We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.

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Zhang, R., Li, Q., Xiao, J. et al. Characterization of a Homeodomain-Leucine Zipper Gene 12: Gene Silencing in Pepper and Arabidopsis-Based Overexpression During Abiotic Stress. J Plant Growth Regul 43, 1689–1706 (2024). https://doi.org/10.1007/s00344-023-11215-5

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