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Synergistic Effects of Selenium and Silicon Mitigate Arsenic Toxicity in Oryza sativa L.

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Abstract

Arsenic (As) is a toxic element for plants, animals and humans. The mitigation of As stress has been achieved via selenium (Se) and silica (Si) supplementations. However, the effects of combined application of Se and Si against As stress are not known. The present study was conducted to evaluate the effect of Se (0.5 and 1 mg L−1) and Si (10 and 30 mg L−1) on Oryza sativa L. (rice) subjected to during As (4 mg L−1) stress. The rice plants showed reduction in As accumulation in co-treatment of As + Se, As + Si and As + Se + Si in comparison to As alone treated plants (7 d). The maximum reduction of As accumulation in root and shoot was 57% and 64%, respectively in As + Se + Si treatment. The increase in enzymatic antioxidant system (superoxide dismutase, ascorbate peroxidase, glutathione peroxidase, glutathione reductase and glutathione-s-transferase) along with decrease in oxidative stress markers (lipid peroxidation, dehydroascorbate reductase, ascorbate oxidase and hydrogen peroxide) in As + Se + Si treatment as compared to As treatment signified elevated tolerance of rice plants to As stress. Gene expressions of enzymes involved in antioxidant defence and thiol metabolism were found to be significantly positively correlated with their respective biochemical activities. In conclusion an optimum combination of Se and Si can be used to effectively mitigate As toxicity in plants.

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Acknowledgements

The authors are thankful to Botany Department, Lucknow University, Lucknow for the facilities. Amit Kumar is thankful to SERB, DST, New Delhi for the award and financial assistance in form of SERB-NPDF.

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Authors and Affiliations

  1. Department of Botany, University of Lucknow, Lucknow, India

    Amit Kumar, Mohammad Israil Ansari & Abu Baker

  2. Council of Scientific and Industrial Research—National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India

    Pradyumna Kumar Singh

  3. Feinberg School of Medicine, Northwestern University, Chicago, USA

    Abu Baker

  4. Department of Botany, Siddharth University, Kapilvastu, Uttar Pradesh, 272202, India

    Kiran Gupta

  5. Plant Stress Biology Laboratory, Institute of Environment and Sustainable Development, Banaras Hindu University, Varanasi, 221005, India

    Sudhakar Srivastava

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AK performed experimental work, prepared figures and writing original draft. SS, MIA and KG- writing, review & editing. AB and PKS helped in biochemical analysis. All authors have read and approved the manuscript.

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Kumar, A., Ansari, M.I., Singh, P.K. et al. Synergistic Effects of Selenium and Silicon Mitigate Arsenic Toxicity in Oryza sativa L.. J Plant Growth Regul 43, 1272–1286 (2024). https://doi.org/10.1007/s00344-023-11182-x

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