Abstract
Sustainable agriculture is a key factor to meet the global demand of food supply. In recent years, nanotechnology has been adopted as a promising tool for achieving the sustainable agriculture. The advent of nanotechnology may play a significant role from farm to fork, by improving the yield and quality. Several approaches are utilized viz nano-farming, nanoparticles-enabled smart delivery system, nanofertilizers, nanomaterials to enhance the quality by promoting the seed germination, increasing biochemical constituents, adaptation and resistance to diseases. Polyherbal nanoformulation (PHNF) was formulated using green nanotechnology with the assistance of triphala extract and silver nitrate solution. Synthesized PHNF was physico chemically confirmed for the formation of nanosized particles. This research focuses on applications of polyherbal nanoformulation (PHNF) on plant growth promotion in Vigna radiata and Trigonella foenum-graecum. Germinating seeds were sprayed with 1 ppm, 5 ppm, 10 ppm of PHNF and compared with the control. Upon PHNF treatment the growth parameters including shoot length, root length and fresh weight were increased in all two tested species. There was a gradual increase in the photosynthetic pigments including total chlorophyll, chlorophyll a, chlorophyll b, carotenoids and biochemical constituents including proteins and carbohydrates and is concentration dependent. These results suggested that polyherbal nanoformulation can be used as an alternative to chemical fertilizers to overcome the drawbacks of synthetic fertilizers and to provide eco-friendly solution for plants and protect the ecosystem from hazardous chemicals.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Data availability
Data will be available on request.
Code availability
Not Applicable.
References
Acharya P, Jayaprakasha GK, Crosby KM et al (2020) Nanoparticle-mediated seed priming improves germination, growth, yield, and quality of watermelons (Citrullus lanatus) at multi-locations in texas. Sci Rep 10:5037. https://doi.org/10.1038/s41598-020-61696-7
Almutairi Z, Alharbi A (2015) Effect of silver nanoparticles on seed germination of crop plants. J Adv Agri. 4(1):280–285. https://doi.org/10.24297/jaa.v4i1.4295
Aron D (1949) Copper enzymes isolated chloroplasts, polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15
Aslani F, Bagheri S, Muhd Julkapli N, Juraimi AS, Hashemi FS, Baghdadi A (2014) Effects of engineered nanomaterials on plants growth: an overview. Sci World J 2014:641759. https://doi.org/10.1155/2014/641759
Babaei M, Shabani L, Hashemi-Shahraki S (2022) Improving the effects of salt stress by β-carotene and gallic acid using increasing antioxidant activity and regulating ion uptake in Lepidium sativum L. Bot Stud 63:22. https://doi.org/10.1186/s40529-022-00352-x
Basheerudeen MA, Mushtaq S, Soundhararajan R, Nachimuthu SK, Srinivasan H (2020) Marine endophytic fungi mediated Silver nanoparticles and their application in plant growth promotion in Vigna radiata L. Int J Nano Dimens 12(1):1–10
Biba R, Košpić K, Komazec B, Markulin D, Cvjetko P, Pavoković D, Peharec Štefanić P, Tkalec M, Balen B (2022) Surface Coating-Modulated Phytotoxic Responses of Silver Nanoparticles in Plants and Freshwater Green Algae. Nanomaterials 12(1):24. https://doi.org/10.3390/nano12010024
Budhani S, Egboluche NP, Arslan Z, Yu H, Deng H (2019) Phytotoxic effect of silver nanoparticles on seed germination and growth of terrestrial plants. J Environm Sci Health. 37(4):330–355. https://doi.org/10.1080/10590501.2019.1676600
Burke DJ, Pietrasiak N, Situ SF, Abenojar EC, Porche M, Kraj P, Lakliang Y, Samia AC (2015) Iron oxide and titanium dioxide nanoparticle effects on plant performance and root associated microbes. Int J Mol Sci 16(10):23630–23650. https://doi.org/10.3390/ijms161023630
Davarpanaha O, Tehranifara A, Davarynejada G, Abadíab J, Khorasanic E (2016) Effects of foliar applications of zinc and boron nano-fertilizers on pomegranate (Punica granatum cv. Ardestani) fruit yield and quality. Sci Hortic 210:57–64. https://doi.org/10.1016/j.scienta.2016.07.003
Farghaly FA, Nafady NA (2015) Green synthesis of silver nanoparticles using leaf extract of Rosmarinus officinalis and its effect on Tomato and wheat plants. J Agric Sci. https://doi.org/10.5539/jas.v8n4p179
Juárez-Maldonado A, Ortega-Ortíz H, Morales-Díaz A, González-Morales S, Morelos-Moreno Á, Sandoval-Rangel A, Cadenas-Pliego G, Benavides-Mendoza A (2019) Nanoparticles and nanomaterials as plant biostimulants. Int J Mol Sci 20:162
Khan MS, Ranjani S (2022) Synthesis and characterization of Kappaphycus alvarezii derived silver nanoparticles and determination of antibacterial activity. Mater Chem Phys 282:125985. https://doi.org/10.1016/j.matchemphys.2022.125985
Khan A, Nazar S, Lang I, Nawaz H, Hussain MA (2017) Effect of ellagic acid on growth and physiology of canola (Brassica napus L) under saline conditions. J Plant Interact. 12(1):520–525. https://doi.org/10.1080/17429145.2017.1400122
Khan MS, Pandey MK, Hemalatha S (2018) Comparative studies on the role of organic biostimulant in resistant and susceptible cultivars of rice grown under saline stress - organic biostimulant alleviate saline stress in tolerant and susceptible cultivars of rice. J Crop Sci Biotechnol 21:459–467
Khan M, Akther T, MubarakAli D, Hemalatha S (2019a) An investigation on the role of salicylic acid alleviate the saline stress in rice crop (Oryza sativa (L)). Agric. Biotechnol, Biocatal. https://doi.org/10.1016/j.bcab.2019.101027
Khan MS, Uandai SB, Hemalatha S (2019b) Anthracnose disease diagnosis by image processing, support vector machine and correlation with pigments. J Plant Pathol 101:749–751. https://doi.org/10.1007/s42161-019-00268-9
Li Z, Juneau P, Lian Y, Zhang W, Wang S, Wang C, Shu L, Yan Q, He Z, Xu K (2020) Effects of Titanium Dioxide Nanoparticles on Photosynthetic and Antioxidative Processes of Scenedesmus obliquus. Plants (basel, Switzerland) 9(12):1748. https://doi.org/10.3390/plants9121748
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent (PDF). J Biol Chem 193(1):265–275. https://doi.org/10.1016/S0021-9258(19)52451-6
Mittal D, Kaur G, Singh P, Yadav K, Ali SA (2020) Nanoparticle-based sustainable agriculture and food science: recent advances and future outlook. Front Nanotechnol. 2:579954. https://doi.org/10.3389/fnano.2020.579954
Noshad A, Iqbal M, Folkers LC, Hetherington CJ, Khan A, Numan M, Ullah S (2019) Antibacterial effect of silver nanoparticles (agnps) synthesized from trichoderma harzianum against Clavibacter Michiganensis. J Nano Res 58:10–19
Olanrewaju OS, Glick BR, Babalola OO (2017) Mechanisms of action of plant growth promoting bacteria. World J Microbiol Biotechnol 33(11):197. https://doi.org/10.1007/s11274-017-2364-9
Parray JA, Kamili AN, Reshi ZA, Hamid R, Qadri R (2013) Screening of beneficial properties of rhizobacteria isolated from Saffron (Crocus sativus L) rhizosphere. Afr J Microbiol Res 7:2905–2910
Racuciu M, Creanga DE (2007) TMA-OH Coated magnetic nanoparticles internalized in vegetal tissue. Roman J Phys 52:395–402
Ranjani S, Hemalatha S (2022) Triphala decorated nanoparticles with multi potent properties. Mater Lett 308:131184. https://doi.org/10.1016/j.matlet.2021.131184
Ranjani S, Shruthy Priya P (2021b) Novel polyherbal nanocolloids to control bovine mastitis. Appl Biochem Biotechnol 194:246–265. https://doi.org/10.1007/s12010-021-03748-w
Ranjani S, Das R, Shariq Ahmed M, Lalnunmawii E, Senthilkumar N, Ruckmani K, Hemalatha S (2020) Myco-nanocolloids manipulate growth biofilm formation and virulence genes in UTI causing E. Coli Inorg Nano-Met Chem. 51:1725–1734. https://doi.org/10.1080/24701556.2020.1852426
Ranjani S, Noorul Samsoon Maharifa H, Raihanathus Sahdhiyya A, Hemalatha S (2021a) Phytotoxicity assessment of synthesized green nanosuspension on germination and growth in Vigna radiate. Inorg Nano-Met Chem. https://doi.org/10.1080/24701556.2021.1993916
Ranjani S, Parthasarathy P, Rameshkumar P, Hemalatha S (2022) Myrobalan-Mediated Nanocolloids in Controlling Marine Pathogens. Appl Biochem Biotechnol 194(3):1120–1135. https://doi.org/10.1007/s12010-022-03816-9
Rasool U, Sah SK, Hemalatha S (2018) Growth inhibitory effect of oven dried copper nanoparticles (cunps) on drug resistant clinical isolates Ira. J. Mat. Sci. Eng. 15(2):12–20. https://doi.org/10.22068/ijmse.15.3.12
Rastogi A, Zivcak M, Sytar O, Kalaji HM, He X, Mbarki S, Brestic M (2017) Impact of metal and metal oxide nanoparticles on plant: a critical review. Front Chem 5:78. https://doi.org/10.3389/fchem.2017.00078
Sadak MS (2019) Impact of silver nanoparticles on plant growth, some biochemical aspects, and yield of fenugreek plant (Trigonella foenum-graecum). Bull Natl Res Cent 43:38. https://doi.org/10.1186/s42269-019-0077-y
Saidi I, Guesmi F, Kharbech O, Hfaiedh N, Djebali W (2021) Gallic acid improves the antioxidant ability against cadmium toxicity: Impact on leaf lipid composition of sunflower (Helianthus annuus) seedlings. Ecotoxicol Environ Saf 210:111906. https://doi.org/10.1016/j.ecoenv.2021.111906
Salachna P, Byczyńska A, Zawadzińska A, Piechocki R, Mizielińska M (2019) Stimulatory effect of silver nanoparticles on the growth and flowering of potted oriental lilies. Agronomy 9:610
Shaikhaldein HO, Al-Qurainy F, Nadeem M, Khan S, Tarroum M, Salih AM (2020) Biosynthesis and characterization of silver nanoparticles using Ochradenus arabicus and their physiological effect on Maerua oblongifolia raised in vitro. Sci Rep 10(1):17569. https://doi.org/10.1038/s41598-020-74675-9
Shang Y, Hasan MK, Ahammed GJ, Li M, Yin H, Zhou J (2019) Applications of nanotechnology in plant growth and crop protection: a review. Molecules (basel, Switzerland) 24(14):2558. https://doi.org/10.3390/molecules24142558
Siddiqi KS, Husen A (2017) Plant response to engineered metal oxide nanoparticles. Nanoscale Res Lett 12(1):92. https://doi.org/10.1186/s11671-017-1861-y
Singh P, Singh AP (2021) Nanomaterials in Soil Health Management and Crop Production: Potentials and Limitations. In: Kharissova OV, Martínez LMT, Kharisov BI (eds) Handbook of Nanomaterials and Nanocomposites for Energy and Environmental Applications. Springer, Cham
Singh A, Gupta R, Pandey R (2017) Exogenous application of rutin and gallic acid regulate antioxidants and alleviate reactive oxygen generation in Oryza sativa L. Physiol Mol Biol Plants 23:301–309. https://doi.org/10.1007/s12298-017-0430-2
Spinoso-Castillo JL, Chavez-Santoscoy RA, Bogdanchikova N (2017) Antimicrobial and hormetic effects of silver nanoparticles on in vitro regeneration of vanilla (Vanilla planifolia Jacks. ex Andrews) using a temporary immersion system. Plant Cell Tissue Organ Culture 129:195–207. https://doi.org/10.1007/s11240-017-1169-8
Szőllősi R, Molnár Á, Kondak S, Kolbert Z (2020) Dual effect of nanomaterials on germination and seedling growth: stimulation vs phytotoxicity. Plants 9(12):1745. https://doi.org/10.3390/plants9121745
Wagi S, Ahmed A (2019) Green production of AgNPs and their phytostimulatory impact. Green Process Synth 8(1):885–894. https://doi.org/10.1515/gps-2019-0059
Wójtowicz A, Oniszczuk A, Oniszczuk T, Kocira S, Wojtunik K, Mitrus M, Kocira A, Widelski J, Skalicka-Woźniak K (2017) Application of Moldavian dragonhead (Dracocephalum moldavica L) leaves addition as a functional component of nutritionally valuable corn snacks. J Food Sci Technol 54(10):3218–3229. https://doi.org/10.1007/s13197-017-2765-7
Yemm EW, Willis AJ (1954) The estimation of carbohydrates in plant extracts by anthrone. Biochem J 57(3):508–514. https://doi.org/10.1042/bj0570508
Acknowledgements
Authors are thankful to B.S. Abdur Rahman Institute of Science and Technology, Chennai for providing research facilities in school of life sciences. The authors also gratefully acknowledge the Ministry of Science and Technology, Department of Science and Technology (KIRAN Division) (GoI), New Delhi. (Ref No. DST/WOS-B/2018/1583-HFN (G))
Funding
Ministry of Science and Technology, Department of Science and Technology (KIRAN Division) (GoI), New Delhi. (Ref No. DST/WOS-B/2018/1583-HFN (G)).
Author information
Authors and Affiliations
Contributions
SH conceived and designed research. SR conducted experiments. SH analyzed data. All authors wrote the manuscript. All authors read and approved the manuscript.
Corresponding author
Ethics declarations
Conflicts of interest
The authors declare that there is no conflict of interest.
Ethical approval
Not Applicable.
Consent to participate
Not Applicable.
Consent for publication
All authors read and approved the manuscript for publication.
Additional information
Handling Editor: Rhonda Peavy.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Ranjani, S., Hemalatha, S. Influence of Polyherbal Nanoformulation on Plant Growth and Biochemical Constituents in Legume Seedlings. J Plant Growth Regul 42, 7142–7150 (2023). https://doi.org/10.1007/s00344-023-11003-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00344-023-11003-1