Abstract
The papain-like cysteine proteinases (PLCPs) are very stable proteolytic enzymes with the pH dependency and substrate specificity, which participate in the autophagy pathway and are usually found in apoplast, vacuole, and lysosome. However, limited information about the PLCP genes in grapes is available. In this study, according to the conservative domain and evolutionary relationship with Arabidopsis, 22 VviPLCP genes were identified in the grape and subdivided into nine subgroups. Differential expression pattern of the VviPLCP genes indicated that they may be involved in response to various hormonal treatments. Yeast two-hybrid and Biomolecular fluorescence complementation assays showed that VviALP interacted with the Cystatin protein VviCYS6 in grapes. In addition, overexpression of VviALP and VviCYS6 in Nicotiana tabacum leaves found that VviALP played an antagonistic role in the resistance of Phytophthora capsica inhibited by VviCYS6. Taken together, our findings provide new insights into the molecular mechanism of VviPLCP involving biological progress. It is found that the regulatory modules of VviALP and VviCYS6 play an important role in the resistance of Phytophthora capsici. In future studies, the transgenic grape of VviALP and VviCYS6 can more clearly prove their role in plant disease resistance.Kindly check and confirm hierarchy of the affiliation 2.OK.
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Acknowledgements
This work was supported by the National Key R&D Program of China (2020YFD1000204), the Natural Science Foundation of China (No.32072554), and the Key R&D Program of Shaanxi province (2022-ZDLNY-23).
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CZ and YL conceived and designed the experiments. TZ prepared the figures and wrote the manuscript. JY performed the experiments and analyzed the data. ZQ, LW, and YT analyzed the parts of the data. YL and CZ contributed reagents, materials, and analysis tools.
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Zhao, T., Yan, J., Qu, Z. et al. In Silico and Expression Analysis of Papain-Like Cysteine Proteinases (PLCP) Genes in Vitis vinifera to Predict Their Involvement in Hormonal Signaling and Disease Resistance. J Plant Growth Regul 42, 7070–7082 (2023). https://doi.org/10.1007/s00344-023-10998-x
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DOI: https://doi.org/10.1007/s00344-023-10998-x