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Genome-Wide Identification and Expression Characteristics of Cytokinin Response Factors in Soybean

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Abstract

The cytokinin response factors (CRFs) take crucial part in mediating plant development and stress response processes. However, hardly any information is known about CRF genes in the main crop plant soybean at present. Here, we carried out genome-wide dissection of CRF family in soybean and identified 26 members, which locate on 18 chromosomes of soybean. Sequence information was first observed to gain insight into the soybean CRF genes. Then, the gene duplication, phylogenetic relationships, protein motifs, and gene structure were analyzed in depth to better understand the CRF family in soybean. Cis-regulatory elements analysis showed that these CRFs contain many hormone- and stress-related elements on promoter regions, indicating their potential involvement in response to these factors. In addition, through investigating the public transcriptome data, we studied the expression patterns of those CRFs in different tissues/organs and various environments. The results revealed that expression of soybean CRFs not only exhibited spatial-diversity, but also differs in response to cold, drought, and salt stress. Our research offers new insights into soybean CRFs as well as useful information for future functional analysis of this gene family.

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Data Availability

Data used or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by the Natural Science Foundation of Heilongjiang Province of China (YQ2019C027) and the Research Foundation for Doctors of Liaoning Province (2019-BS-171).

Funding

This work was supported by the Natural Science Foundation of Heilongjiang Province of China (YQ2019C027) and the Research Foundation for Doctors of Liaoning Province (2019-BS-171).

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Authors and Affiliations

  1. Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security, College of Life Science and Engineering, Shenyang University, Shenyang, 110044, China

    Xiangbo Duan, Ke Zhang & Yang Yu

  2. Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, School of Life Sciences, Heilongjiang University, Harbin, 150080, China

    Huizi Duanmu

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  1. Xiangbo Duan
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  2. Ke Zhang
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Contributions

XD and YY conceived and designed the work, XD, KZ, and HD contributed to experiment performance and data analysis, XD and HD wrote the manuscript, KZ and YY revised and edited the manuscript. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Huizi Duanmu or Yang Yu.

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Duan, X., Zhang, K., Duanmu, H. et al. Genome-Wide Identification and Expression Characteristics of Cytokinin Response Factors in Soybean. J Plant Growth Regul 42, 4484–4496 (2023). https://doi.org/10.1007/s00344-023-10914-3

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