Abstract
The YABBY gene family of plant-specific transcription factors plays important roles in the development of lateral organs, the establishment of adaxial-abaxial polarity and abiotic stress response. The YABBY gene family in lettuce (Lactuca sativa), an important leafy vegetable, remains to be identified. In this study, we analyzed the chromosomal distribution, gene structure and cis-transcriptional elements of the YABBY gene family in lettuce. Phylogenetic analysis of YABBY genes in lettuce classified them into four groups. The expression profile of YABBY genes in lettuce indicated that they might exert distinct functions in different tissues and developmental processes. The subcellular localization analysis and transactivation assay showed that LsaFILd probably functions as a transcription factor in the nucleus. Furthermore, the ectopic expression of LsaFILd exhibited leaves curled outwardly, not only in Arabidopsis thaliana but also in lettuce. Altogether, LsaFILd is a key functional gene in lettuce leaf development. Our study laid a foundation for the molecular research of functional genes in lettuce.
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Abbreviations
- MW:
-
Molecular Weight
- ARF:
-
Auxin Response Factor
- DL1:
-
DROOPING LEAF 1
- TCP:
-
TEOSINTE BRANCHED1, CYCLOIDEA, and PROLIFERATING CELL NUCLEAR ANTIGEN BINDING FACTOR
- FIL:
-
FILAMENTOUS FLOWER
- INO:
-
INNER NO OUTER
- CRC:
-
CRABS CLAW
- TF:
-
Transcription Factor
- MS:
-
Murashige and Skoog
- pI:
-
Isoelectric point
- qRT–PCR:
-
Quantitative real-time polymerase chain reaction
- GRF:
-
Growth-Regulating Factor
- AbA:
-
Aureobasidin A
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Acknowledgements
This work was supported by the Beijing Academy of Agriculture and Forestry Science (KJCX20220104) and the Innovation and Development Programs of Beijing Vegetable Research Center (KYCX202002-01 and KYCX201901-01). The funding was used for performing experiments and paying salaries of researchers. We would like to thank Drs. Tianhua He, Zhenying Shi, Maoyin Li, and Man Zhou for their critical reading of the manuscript.
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DL, DZ, JZ, and BZ designed experiments. KL, ZZ, and BZ identified and characterized the YABBY genes family in lettuce. KL, ZZ, and XL carried out the experiment. KL, BZ, and DL wrote the paper. All authors discussed the results and commented on the manuscript.
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Luo, K., Zhang, D., Zhai, Z. et al. Genome-Wide Analysis of YABBY Gene Family in Lettuce (Lactuca sativa) and Functional Characterization of LsaFILd. J Plant Growth Regul 42, 2124–2135 (2023). https://doi.org/10.1007/s00344-022-10687-1
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DOI: https://doi.org/10.1007/s00344-022-10687-1