Abstract
Pectin is a natural anionic polysaccharide, which is of great significance in agricultural applications, but its solubility in water limits its application. Pectin nanospheres can improve the dispersion of pectin in water. This work is to study the effects of pectin nanospheres on wheat germination and seedling growth. The pectin nanospheres were synthesized by ionotropic gelation. Meanwhile, the morphology of pectin nanospheres and the attachment on the surface of wheat seeds coat were characterized. The different concentrations (50, 100, 500 μg/mL) of pectin nanospheres were used to research the impact of wheat seed germination. In the concentration of 50 μg/mL pectin nanospheres treatment groups, the germination rate and seedling index got a 15.6% and 13.9% increase, respectively. The germination index increased by 22.1%, fresh weight of the aboveground part and underground part, respectively, increased by 26.7% and 17.3%, dry weight of the aboveground part and underground part, respectively, increased by 9.65% and 9.97%, and number of adventitious roots and the length of seedlings went up by 36.6% and 20.1%, respectively. Moreover, in seedlings stage the 100 μg/mL promoted the net photosynthetic rate of wheat by 60.2% and uptake of nutrient. In addition, energy-dispersive spectroscopy (EDS) confirmed the adsorption of pectin nanospheres on the surface of wheat seeds.
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References
Al-Asmar A, Giosafatto CVL, Sabbah M, Sanchez A, Villalonga Santana R, Mariniello L (2019) Effect of mesoporous silica nanoparticles on the physicochemical properties of pectin packaging material for strawberry wrapping. Nanomaterials. https://doi.org/10.3390/nano10010052
Arora S, Sharma P, Kumar S, Nayan R, Khanna PK, Zaidi MGH (2012) Gold-nanoparticle induced enhancement in growth and seed yield of Brassica juncea. Plant Growth Regul 66:303–310. https://doi.org/10.1007/s10725-011-9649-z
Bettani SR, de Oliveira Ragazzo G, Leal Santos N, Kieckbusch TG, Gaspar Bastos R, Soares MR, da Silva MA (2019) Sugarcane vinasse and microalgal biomass in the production of pectin particles as an alternative soil fertilizer. Carbohydr Polym 203:322–330. https://doi.org/10.1016/j.carbpol.2018.09.041
Bloch D, Hoffmann CM, Märländer B (2006) Impact of water supply on photosynthesis, water use and carbon isotope discrimination of sugar beet genotypes. Eur J Agron 24:218–225. https://doi.org/10.1016/j.eja.2005.08.004
Bostanudin MF, Arafat M, Sarfraz M, Gorecki DC, Barbu E (2019) Butylglyceryl pectin nanoparticles: synthesis formulation and characterization. Polymers. https://doi.org/10.3390/polym11050789
Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254. https://doi.org/10.1006/abio.1976.9999
Camejo D, Marti MC, Jimenez A, Cabrera JC, Olmos E, Sevilla F (2011) Effect of oligogalacturonides on root length, extracellular alkalinization and O2– accumulation in alfalfa. J Plant Physiol 168:566–575. https://doi.org/10.1016/j.jplph.2010.09.012
Chittasupho C, Jaturanpinyo M, Mangmool S (2013) Pectin nanoparticle enhances cytotoxicity of methotrexate against HepG2 cells. Drug Deliv 20:1–9. https://doi.org/10.3109/10717544.2012.739214
de Araújo Silva MM, Ferreira LT, de Vasconcelos FMT, Willadino L, Camara TR, dos Santos DYAC, de Oliveira AFM (2020) Water stress-induced responses in the growth, cuticular wax composition, chloroplast pigments and soluble protein content, and redox metabolism of two genotypes of Ricinus communis L. J Plant Growth Regul. https://doi.org/10.1007/s00344-020-10103-6
Garna H, Mabon N, Nott K, Wathelet B, Paquot M (2006) Kinetic of the hydrolysis of pectin galacturonic acid chains and quantification by ionic chromatography. Food Chem 96:477–484. https://doi.org/10.1016/j.foodchem.2005.03.002
Gawkowska D, Cybulska J, Zdunek A (2018) Structure-related gelling of pectins and linking with other natural compounds: a review. Polymers. https://doi.org/10.3390/polym10070762
Gruskiene R, Krivorotova T, Sereikaite J (2017) Nisin-loaded pectin and nisin-loaded pectin-inulin particles: comparison of their proteolytic stability with free nisin. LWT Food Sci Technol 82:283–286. https://doi.org/10.1016/j.lwt.2017.04.061
Guan Y, Hu J, Wang X, Shao C (2009) Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. J Zhejiang Univ Sci B 10:427–433. https://doi.org/10.1631/jzus.B0820373
Gunter EA, Markov PA, Melekhin AK, Belozerov VS, Martinson EA, Litvinets SG, Popov SV (2018) Preparation and release characteristics of mesalazine loaded calcium pectin-silica gel beads based on callus cultures pectins for colon-targeted drug delivery. Int J Biol Macromol 120:2225–2233. https://doi.org/10.1016/j.ijbiomac.2018.07.078
Huang B et al (2018) Advances in targeted pesticides with environmentally responsive controlled release by nanotechnology. Nanomaterials. https://doi.org/10.3390/nano8020102
Jiang J et al (2014) Effect of cold plasma treatment on seed germination and growth of wheat plasma. Sci Technol 16:54–58. https://doi.org/10.1088/1009-0630/16/1/12
Kananont N, Pichyangkura R, Chanprame S, Chadchawan S, Limpanavech P (2010) Chitosan specificity for the in vitro seed germination of two Dendrobium orchids (Asparagales: Orchidaceae). Sci Horticult 124:239–247. https://doi.org/10.1016/j.scienta.2009.11.019
Kanokporn Burapapadh MK-V, Doungdaw C, Pornsak S (2010) Fabrication of pectin-based nanoemulsions loaded with itraconazole for pharmaceutical application. Carbohydr Polym 82:384–393. https://doi.org/10.1016/j.carbpol.2010.04.071
Kaya M, Kaya G, Kaya MD, Atak M, Saglam S, Khawar KM, Ciftci CY (2008) Interaction between seed size and NaCl on germination and early seedling growth of some Turkish cultivars of chickpea (Cicer arietinum L.). J Zhejiang Univ Sci B 9:371–377. https://doi.org/10.1631/jzus.B0720268
Kurita O, Miyake Y, Yamazaki E (2012) Chemical modification of citrus pectin to improve its dissolution into water. Carbohydr Polym 87:1720–1727. https://doi.org/10.1016/j.carbpol.2011.09.081
Li R et al (2019) Effects of chitosan nanoparticles on seed germination and seedling growth of wheat (Triticum aestivum L.). Int J Biol Macromol 126:91–100. https://doi.org/10.1016/j.ijbiomac.2018.12.118
Liu H, Zhang YH, Yin H, Wang WX, Zhao XM, Du YG (2013) Alginate oligosaccharides enhanced Triticum aestivum L. tolerance to drought stress. Plant Physiol Biochem 62:33–40. https://doi.org/10.1016/j.plaphy.2012.10.012
Lu J, Li J, Jin R, Li S, Yi J, Huang J (2019) Extraction and characterization of pectin from Premna microphylla Turcz leaves. Int J Biol Macromol 131:323–328. https://doi.org/10.1016/j.ijbiomac.2019.03.056
Ma Y, Zhou L, Wang Z, Chen J, Qu G (2016) Oligogalacturonic acids promote tomato fruit ripening through the regulation of 1-aminocyclopropane-1-carboxylic acid synthesis at the transcriptional and post-translational levels. BMC Plant Biol 16:13. https://doi.org/10.1186/s12870-015-0634-y
Mahendra C, Chandra MN, Murali M, Abhilash MR, Singh SB, Satish S, Sudarshana MS (2020) Phyto-fabricated ZnO nanoparticles from Canthium dicoccum (L.) for antimicrobial, anti-tuberculosis and antioxidant activity. Process Biochem 89:220–226. https://doi.org/10.1016/j.procbio.2019.10.020
Meng X, Gong Y, Fan H, Yu Y (2013) Photosynthesis regulation by glucohexaose through redox changes in Cucumis sativus. J Plant Growth Regul 33:571–578. https://doi.org/10.1007/s00344-013-9405-x
Mohnen D (2008) Pectin structure and biosynthesis. Curr Opin Plant Biol 11:266–277. https://doi.org/10.1016/j.pbi.2008.03.006
Mukherjee A et al (2016) Differential toxicity of bare and hybrid ZnO nanoparticles in green pea (Pisum sativum L.): A Life Cycle Study. Front Plant Sci. https://doi.org/10.3389/fpls.2015.01242
Ngouémazong DE et al (2012) Effect of de-methylesterification on network development and nature of Ca2+-pectin gels: towards understanding structure–function relations of pectin. Food Hydrocolloids 26:89–98. https://doi.org/10.1016/j.foodhyd.2011.04.002
Oljira AM et al (2020) Trichoderma enhances net photosynthesis, water use efficiency, and growth of wheat (Triticum aestivum L.) under salt stress. Microorganisms. https://doi.org/10.3390/microorganisms8101565
Opanasopit P, Apirakaramwong A, Ngawhirunpat T, Rojanarata T, Ruktanonchai U (2008) Development and characterization of pectinate micro/nanoparticles for gene delivery. AAPS PharmSciTech 9:67–74. https://doi.org/10.1208/s12249-007-9007-7
Pereira AES, Sandoval-Herrera IE, Zavala-Betancourt SA, Oliveira HC, Ledezma-Perez AS, Romero J, Fraceto LF (2017) gamma-Polyglutamic acid/chitosan nanoparticles for the plant growth regulator gibberellic acid: characterization and evaluation of biological activity. Carbohydr Polym 157:1862–1873. https://doi.org/10.1016/j.carbpol.2016.11.073
Presentato A et al (2020) Superior antibacterial activity of integral lemon pectin extracted via hydrodynamic cavitation. ChemistryOpen 9:628–630. https://doi.org/10.1002/open.202000076
Rashidipour M et al (2019) Pectin/chitosan/tripolyphosphate nanoparticles: efficient carriers for reducing soil sorption, cytotoxicity and cutagenicity of paraquat and enhancement of its herbicide activity. J Agric Food Chem. https://doi.org/10.1021/acs.jafc.9b01106
Rui M et al (2016) Iron oxide nanoparticles as a potential iron fertilizer for peanut (Arachis hypogaea). Front Plant Sci. https://doi.org/10.3389/fpls.2016.00815
Saharan V, Kumaraswamy RV, Choudhary RC, Kumari S, Pal A, Raliya R, Biswas P (2016) Cu-Chitosan nanoparticle mediated sustainable approach to enhance seedling growth in maize bymobilizing reserved. J Agric Food Chem 64:6148–6155. https://doi.org/10.1021/acs.jafc.6b02239
Sathiyabama M, Manikandan A (2016) Chitosan nanoparticle induced defense responses in fingermillet plants against blast disease caused by Pyricularia grisea (Cke) Sacc. Carbohydr Polym 154:241–246. https://doi.org/10.1016/j.carbpol.2016.06.089
Shajahan A, Shankar S, Sathiyaseelan A, Narayan KS, Narayanan V, Kaviyarasan V, Ignacimuthu S (2017) Comparative studies of chitosan and its nanoparticles for the adsorption efficiency of various dyes. Int J Biol Macromol 104:1449–1458. https://doi.org/10.1016/j.ijbiomac.2017.05.128
Shigehiro Hirano NN (1989) Effects of chitosan pectic acid, lysozyme, and chitinase on the growth of several phytopathogens. Agric Biol Chem 53:3065–3066
Stauber RH, Siemer S, Becker S, Ding GB, Strieth S, Knauer SK (2018) Small meets smaller: effects of nanomaterials on microbial biology, pathology, and ecology. ACS Nano 12:6351–6359. https://doi.org/10.1021/acsnano.8b03241
Sundaria N, Singh M, Upreti P, Chauhan RP, Jaiswal JP, Kumar A (2018) Seed priming with iron oxide nanoparticles triggers iron ccquisition and biofortification in wheat (Triticum aestivum L.). Grains J. Plant Growth Regul. 38:122–131. https://doi.org/10.1007/s00344-018-9818-7
Suzuki T, Tomita-Yokotani K, Yoshida S, Takase Y, Kusakabe I, Hasegawa K (2002) Preparation and isolation of oligogalacturonic acids and their biological effects in cockscomb (Celosia argentea L.) seedlings. J Plant Growth Regul 21:209–215. https://doi.org/10.1007/s003440010060
Terry N (1983) Limiting factors in photosynthesis Iron stress-mediated changes in light-harvesting and electron-transport capacity and its effects on photosynthesis invivo. Plant Physiol 71:855–860. https://doi.org/10.1104/pp.71.4.855
Tombuloglu H, Slimani Y, Tombuloglu G, Korkmaz AD, Baykal A, Almessiere M, Ercan I (2019) Impact of superparamagnetic iron oxide nanoparticles (SPIONs) and ionic iron on physiology of summer squash (Cucurbita pepo): a comparative study. Plant Physiol Biochem 139:56–65. https://doi.org/10.1016/j.plaphy.2019.03.011
Tombuloglu H, Ercan I, Alshammari T, Tombuloglu G, Slimani Y, Almessiere M, Baykal A (2020a) Incorporation of micro-nutrients (nickel, copper, zinc, and iron) into plant body through nanoparticles. J Soil Sci Plant Nutr 20:1872–1881. https://doi.org/10.1007/s42729-020-00258-2
Tombuloglu H et al (2020b) Magnetic behavior and nutrient content analyses of barley (Hordeum vulgare L.) tissues upon CoNd0.2Fe1.8O4 magnetic nanoparticle treatment. J Soil Sci Plant Nutr 20:357–366. https://doi.org/10.1007/s42729-019-00115-x
Tombuloglu H et al (2020c) Engineered magnetic nanoparticles enhance chlorophyll content and growth of barley through the induction of photosystem genes. Environ Sci Pollut Res 27:34311–34321. https://doi.org/10.1007/s11356-020-09693-1
Venkatachalam P et al (2017) Enhanced plant growth promoting role of phycomolecules coated zinc oxide nanoparticles with P supplementation in cotton (Gossypium hirsutum L). Plant Physiol Biochem 110:118–127. https://doi.org/10.1016/j.plaphy.2016.09.004
Weraduwage SM, Kim S-J, Renna L, Anozie FC, Sharkey TD, Brandizzi F (2016) Pectin methylesterification impacts the relationship between photosynthesis and plant growth. Plant Physiol 171:833–848. https://doi.org/10.1104/pp.16.00173
Yang S-H, Wang L-J, Li S-H, Duan W, Loescher W, Liang Z-C (2007) The effects of UV-B radiation on photosynthesis in relation to Photosystem II photochemistry, thermal dissipation and antioxidant defenses in winter wheat (Triticum aestivum L.) seedlings at different growth temperatures. Funct Plant Biol 34:907–917. https://doi.org/10.1071/fp07083
Yu CY, Cao H, Zhang XC, Zhou FZ, Cheng SX, Zhang XZ, Zhuo RX (2009) Hybrid nanospheres and vesicles based on pectin as drug carriers. Langmuir 25:11720–11726. https://doi.org/10.1021/la901389v
Zainudin BH, Wong TW, Hamdan H (2018) Design of low molecular weight pectin and its nanoparticles through combination treatment of pectin by microwave and inorganic salts. Polym Degrad Stab 147:35–40. https://doi.org/10.1016/j.polymdegradstab.2017.11.011
Zhang DY, Chen Y, Xu D-Q (2005) Determinant of photosynthetic capacity in rice leaves under ambient air conditions. Photosynthetic 43:273–276
Zhang S, Hu J, Zhang Y, Xie XJ, Knapp A (2007) Seed priming with brassinolide improves lucerne (Medicago sativa L.) seed germination and seedling growth in relation to physiological changes under salinity stress. Austral J Agric Res 58:811. https://doi.org/10.1071/ar06253
Zhang W, Xu P, Zhang H (2015) Pectin in cancer therapy: a review. Trends Food Sci Technol 44:258–271. https://doi.org/10.1016/j.tifs.2015.04.001
Acknowledgements
This work was supported by the Key Collaborative Research Programme of the Alliance of International Science Organizations (Grant No. ANSO-CR-KP-2020-14), Dalian Science and Technology Innovation Fund-Key & Major Subject (2020JJ25CY017), and Special Foundation for The Central Government to guide Local Scientific and Technological Development (2021JH6/10500111), and G. C-B was funded by ANID CONICYT PIA/APOYO CCTE AFB170007. Dr. Heng Yin was supported by Liaoning Revitalization Talents Programme (XLYC1807041).
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HY designed the experiment and contributed to the conception of the study, RL and ML performed the experiment and the data analyses and wrote the manuscript, HX, WW, MZ, GC-B, and PM helped perform the analysis with constructive discussions.
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Li, R., Li, M., He, J. et al. Preparation of Pectin Nanospheres and Its Effect on Wheat (Triticum aestivum L.) Seed Germination and Growth. J Plant Growth Regul 41, 3197–3207 (2022). https://doi.org/10.1007/s00344-021-10505-0
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DOI: https://doi.org/10.1007/s00344-021-10505-0