Abstract
To investigate the effects of glucohexaose (P6) on cucumber, leaf CO2 assimilation, chlorophyll fluorescence parameters, chlorophyll content, and carbohydrate metabolism were examined in cucumber plants. The net photosynthetic rate (P n ) of cucumber leaves was enhanced after being treated with 10 μg mL−1 P6. The increase was correlated with increases in transpiration rate (E) and stomatal conductance (G s), whereas the intercellular CO2 concentration (C i) was not different from the control plants. Chlorophyll content, absorption of light energy per unit area (ABS/CS), capture of light energy per unit area (TRo/CS), quantum yield of electron transport per unit area (ETo/CS), maximum photochemical efficiency of PSII (φP o), quantum yield of photosynthetic institution electron transfer (φE o), probability of other electron acceptors that captured exciton-transferred electrons to the electronic chain which exceeds QA (ψ o), number of reaction centers per unit leaf area (RC/CSo), and the performance index on absorption basis (PIABS) were improved, but heat dissipation per unit area (DIo/CS) and maximum quantum yield of non-chemical quenching (φD o) were reduced. In addition, increases in sucrose, soluble sugars, and starch contents were observed in P6-treated plants. However, H2O2 scavenger (DMTU) or NADPH oxidase inhibitor (DPI) pretreatment significantly abolished the effect of P6 on photosynthesis. The results demonstrated that ROS played a critical role in P6-induced photosynthesis. The increase in chlorophyll content together with efficient light absorption, transmission, and conversion in P6-treated plants is important for increasing photosynthesis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Camejo D, Marti MC, Jimenez A, Cabrera JC, Olmos E, Sevilla F (2011) Effect of oligogalacturonides on root length, extracellular alkalinization and O2−-accumulation in alfalfa. J Plant Physiol 168:566–575
Darvill A, Augur C, Bergmann C, Carlson RW, Cheong JJ, Eberhard S, Hahn MG, LÓ VM, Marfa V, Meyer B, Mohnen D, ONeill MA, Spiro MD, van Halbeek H, York WS, Albersheim P (1992) Oligosaccharins-oligosaccharides that regulate growth, development and defence responses in plants. Glycobiology 2(3):181–198
Diogo RVC, Wydra K (2007) Silicon-induced basal resistance in tomato against Ralstonia solanacearum is related to modification of pectic cell wall polysaccharide structure. Physiol Mol Plant Pathol 70:120–129
Fan HY, Li BJ, Lv CM, Li TL, Zhou BL (2003) Study on cucumber plan: resistance introduced by glucohexaose against Pseudoperonospora cubensis disease. Plant Prot 29(1):14–16
Fan HY, Qu GF, Li TL, Li BJ, Shao MN (2006) Effects of glucohexaose on growth and relative physiological characters of cucumber seedlings. China Vegetables 1:18–20
Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930
Hao YH, Wu CF, Zhao DW, Thung L, YU Y, FAN HY (2013) Proteomic analysis of glucohexaose induced resistance to downy mildew in Cucumis sativus. Aust J Crop Sci 7(9):1242–1251
Jia Z, Zou B, Wang X, Qiu J, Ma H, Gou Z, Song S, Dong H (2010) Quercetin-induced H2O2 mediates the pathogen resistance against Pseudomonas syringae pv. Tomato DC3000 in Arabidopsis thaliana. Biochem Biophys Res Commun 396:522–527
Jiang YP (2010) Brassinosteroids regulate CO2 assimilation through redox ehanges in Cucumis sativus. Doctoral Dissertation of Zhejiang University, Zhejiang
Jiang YP, Cheng F, Zhou YH, Xia XJ, Shi K, Yu JQ (2012a) Interactive effects of CO2 enrichment and Brassinosteroid on CO2 assimilation and photosynthetic electron transport in Cucumis sativus. Environ Exp Bot 75:98–106
Jiang YP, Huang LF, Cheng F, Zhou YH, Xia XJ, Mao WH, Shi K, Yu JQ (2012b) Brassinosteroids accelerate recovery of photosynthetic apparatus from cold stress by balancing the electron partitioning, carboxylation and redox homeostasis in cucumber. Physiol Plant 24:1399–3054
Jindřichová B, Fodor J, Šindelářová L, Burketová L, Valentová O (2011) Role of hydrogen peroxide and antioxidant enzymes in the interaction between a hemibiotrophic fungal pathogen, Leptosphaeria maculans, and oilseed rape. Environ Exp Bot 72:149–156
Khan WM, Prithiviraj B, Smith DL (2002) Effect of foliar application of chitin and chitosan oligosaccharides on photosynthesis of maize and soybean. Photosynthetica 40(4):621–624
Li H-X, Han X-Y, Kang L-J, Ma Z-Q, Zhang X-F, Wang W-Q, Ning J (2004) The primary study of glucohexaose induced resistance to tobacco mosaic virus. Chin J Pest Sci 6:38–42
Mandal S, Mallick N, Mitra A (2009) Salicylic acid-induced resistance to Fusarium oxysporum f. sp. Lycopersici in tomato. Plant Physiol Biochem 47:642–649
Mittler R, Vanderauwera S, Suzuki N, Miller G, Tognetti VB, Vandepoele K, Gollery M, Shulaev V, Van Breusegem F (2011) ROS signaling: the new wave? Trends Plant Sci 16(6):300–309
Montesano M, Brader G, Palva ET (2003) Pathogen derived elicitors: searching for receptors in plants. Mol Plant Pathol 4:73–79
Ozaki K, Uchida A, Takabe T, Shinagawa F, Tanaka Y, Takabe T, Hayashi T, Hattori T, Rai AK, Takabe T (2009) Enrichment of sugar content in melon fruits by hydrogen peroxide treatment. J Plant Physiol 166:569–578
Pereira LB, Mazzanti CM, Gonçalves JF, Cargnelutti D, Tabaldi LA, Becker AG, Calgaroto NS, Farias JG, Battisti V, Bohrer D, Nicoloso FT, Morsch VM, Schetinger MR (2010) Aluminum-induced oxidative stress in cucumber. Plant Physiol Biochem 48:683–689
Shetty NP, Mehrabi R, Lütken H, Haldrup A, Kema GH, Collinge DB, Jørgensen HJ (2007) Role of hydrogen peroxide during the interaction between the hemibiotrophic fungal pathogen Septoria tritici and wheat. New Phytol 174(3):637–647
Tusi SK, Khalaj L, Ashabi G, Kiaei M, Khodagholi F (2011) Alginate oligosaccharide protects against endoplasmic reticulum- and mitochondrial-mediated apoptotic cell death and oxidative stress. Biomaterials 32(23):5438–5458
Vanhoudt N, Vandenhove H, Horemans N, Wannijn J, Bujanic A, Wangronsveld J, Cuypers A (2010) Study of oxidative stress related responses induced in Arabidopsis thaliana following mixed exposure to uranium and cadmium. Plant Physiol Biochem 48:879–886
Wu CF, Hao YH, Pan J, Fan HY (2010) Study on the differential proteomics of cucumber induced by glucohexaose. Acta Hoticulturae Sinica 37:2140
Xia XJ, Huang LF, Zhou YH, Mao WH, Shi K, Wu JX, Asami T, Chen ZX, Yu JQ (2009a) Brassinosteroids promote photosynthesis and growth by enhancing activation of Rubisco and expression of photosynthetic genes in Cucumis sativus. Planta 230:1185–1196
Xia XJ, Zhang Y, Wu JX, Wang JT, Zhou YH, Shi K, Yu YL, Yu JQ (2009b) Brassinosteroids promote metabolism of pesticides in cucumber. J Agric Food Chem 57:8406–8413
Zhang A, Zhang J, Ye N, Cao J, Tan M, Zhang J, Jiang M (2010) ZmMPK5 is required for the NADPH oxidase-mediated self-propagation of apoplastic H2O2 in brassinosteroid-induced antioxidant defence in leaves of maize. J Exp Bot 61:4399–4411
Acknowledgments
This work was supported by the National Nature Science Foundation of China (No. 30700542) and the University Outstanding Young Scholars Growth Project of Liaoning Province (LJQ2011068). The authors are very grateful to Prof. J Ning of Plant Protection Institution of China Agriculture Science Academy for providing glucohexaose.
Author information
Authors and Affiliations
Corresponding author
Additional information
Xiangnan Meng and Yujie Gong are considered as co-first authors with equal contributions.
Rights and permissions
About this article
Cite this article
Meng, X., Gong, Y., Fan, H. et al. Photosynthesis Regulation by Glucohexaose Through Redox Changes in Cucumis sativus . J Plant Growth Regul 33, 571–578 (2014). https://doi.org/10.1007/s00344-013-9405-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00344-013-9405-x