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Isolation and Characterization of Three Plant Growth-Promoting Rhizobacteria for Growth Enhancement of Rice Seedling

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Abstract

Biofertilizers are currently considered the only alternative of chemical fertilizers. In addition, improving the effect of biofertilizers can make chemical fertilizers less demanded, thereby leading to the reduction of adverse environmental effects. In the present study, three rice rhizosphere bacteria (Bacillus megaterium DD-2, Bacillus aryabhattai DD-3 and Bacillus subtilis DD-4) were isolated and then identified from the soil of a farm in Northeast China. As revealed from the results, all isolates exhibited the ability to dissolve potassium and phosphorus. To be specific, DD-4 was capable of dissolving potassium to the greatest extent (1.37 mg/L), DD-2 could maximally dissolve inorganic phosphorus (288.33 mg/L) and DD-3 exhibited the maximum solubility of organic phosphorus (31.58 mg/L). All isolates were capable of synthesizing Indole-3-acetic acid (IAA) (55.66 ~ 75.89 mg/L), Gibberellic acid (GA) (16.33 ~ 23.58 mg/L) and siderophores (38.77 ~ 52.88%). As suggested from the results of the pot experiment, the isolates were capable of promoting the shoot and underground part of rice seedlings. With the increase in the concentration of isolates and the time of addition, the overall effect of the isolates to boost rice seedling growth first increased and then leveled off. The mentioned isolates were observed to exhibit great potential for being biologically applied. However, the impact of isolates on rice required more specific assessments under biotic and abiotic stress before being recommended as biofertilizers.

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Abbreviations

IAA:

Indole-3-acetic acid

GA:

Gibberellic acid

LB:

Luria–Bertani

CAS:

Chrome Azurol S

DD-2:

Bacillus megaterium DD-2

DD-3:

Bacillus aryabhattai DD-3

DD-4:

Bacillus subtilis DD-4

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Acknowledgements

This work was supported by the Major technological innovation program in Hubei Province of China (2019ABA092 and 2019ABA095).

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Authors and Affiliations

  1. Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, National “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Research Center of Food Fermentation Engineering and Technology, Hubei University of Technology, Wuhan, 430068, Hubei, China

    Zeping Liu, Xiaolong Zhang, Leibing Li, Ning Xu, Yong Hu, Chao Wang, Yong Shi & Dongsheng Li

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  1. Zeping Liu
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  2. Xiaolong Zhang
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  3. Leibing Li
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  4. Ning Xu
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  5. Yong Hu
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  6. Chao Wang
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  7. Yong Shi
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  8. Dongsheng Li
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Contributions

ZL, NX, YH, CW, YS and DL designed the study; ZL, XZ, LL and NX performed the research; ZL, NX, YS and DL analyzed the data; and ZL, NX, CW, YS and DL wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Yong Shi or Dongsheng Li.

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Liu, Z., Zhang, X., Li, L. et al. Isolation and Characterization of Three Plant Growth-Promoting Rhizobacteria for Growth Enhancement of Rice Seedling. J Plant Growth Regul 41, 1382–1393 (2022). https://doi.org/10.1007/s00344-021-10393-4

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