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Characterization of Arabidopsis thaliana Root-Related Mutants Reveals ABA Regulation of Plant Development and Drought Resistance

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Abstract

The plant hormone abscisic acid (ABA) regulates many processes of plant growth and development. ABA receptors have been identified in studies of the ABA response of stomatal movement, but the underlying mechanisms of ABA-regulated root growth and development are unclear. To address these questions, we screened for Arabidopsis thaliana mutants based on the sensitivity of root growth to exogenous ABA, using ethyl methanesulfonate-mutagenized (EMS) and T-DNA insertion mutant libraries. We identified 11 mutants, termed roa1–roa11, with Root growth Overly sensitive to ABA (ROA) phenotypes, and cloned two of the ROA genes, one by thermal asymmetric interlaced PCR technology (ROA3) and the other by map-based cloning (ROA9). The roa mutants were also found to have defects in other major ABA responses, including ABA-mediated seed germination and drought resistance. The roa mutants provide crucial genetic material for further studies of ABA signaling and regulatory mechanisms in root growth and development.

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References

  • Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743

    Article  CAS  Google Scholar 

  • Davies WJ, Zhang J (1991) Root signals and the regulation of growth and development of plants in drying soil. Annu Rev Plant Biol 42(1):55–76

    Article  CAS  Google Scholar 

  • De Smet I, Signora L, Beeckman T, Inzé D, Foyer CH, Zhang H (2003) An abscisic acid-sensitive checkpoint in lateral root development of Arabidopsis. Plant J 33:543–555

    Article  Google Scholar 

  • De Smet I, Zhang H, Inzé D, Foyer CH, Zhang H (2006) A novel role for abscisic acid emerges from underground. Trends Plant Sci 11:434–439

    Article  Google Scholar 

  • Dong H, Bai L, Chang J, Song CP (2018a) Chloroplast protein PLGG1 is involved in abscisic acid-regulated lateral root development and stomatal movement in Arabidopsis. Biochem Biophys Res Commun 495:280–285

    Article  CAS  Google Scholar 

  • Dong H, Bai L, Zhang Y, Zhang G, Mao Y, Min L, Xiang F, Qian D, Zhu X, Song C (2018b) Modulation of guard cell turgor and drought tolerance by a peroxisomal acetate–malate shunt. Mol Plant 11:1278–1291

    Article  CAS  Google Scholar 

  • Duan L, Dietrich D, Ng CH, Chan PM, Bhalerao R, Bennett MJ, Dinneny JR (2013) Endodermal ABA signaling promotes lateral root quiescence during salt stress in Arabidopsis seedlings. Plant Cell 25:324–341

    Article  CAS  Google Scholar 

  • Finkelstein RR, Gampala SS, Rock CD (2002) Abscisic acid signaling in seeds and seedlings. Plant Cell 14:s15–s45

    Article  CAS  Google Scholar 

  • Fitter AH (1991) Costs and benefits of mycorrhizas: implications for functioning under natural conditions. Experentia 47:350–355

    Article  Google Scholar 

  • Fujii H, Zhu JK (2009) Arabidopsis mutant deficient in 3 abscisic acid-activated protein kinases reveals critical roles in growth, reproduction, and stress. Proc Natl Acad Sci USA 106:8380–8385

    Article  CAS  Google Scholar 

  • Gonzalez AA, Agbévénou K, Herrbach V, Gough C, Bensmihen S (2015) Abscisic acid promotes pre-emergence stages of lateral root development in Medicago truncatula. Plant Signal Behav 10:1

    Article  Google Scholar 

  • He J, Duan Y, Hua D, Fan G, Wang L, Liu Y, Chen Z, Han L, Qu LJ, Gong Z (2012) DEXH box RNA helicase-mediated mitochondrial reactive oxygen species production in Arabidopsis mediates crosstalk between abscisic acid and auxin signaling. Plant Cell 24:1815–1833

    Article  CAS  Google Scholar 

  • Kramer PJ, Boyer JS (1995) Water relation of plant and soil. Orlando Academic, Orlando

    Google Scholar 

  • Li Y, Zhang L, Lu W, Wang X, Wu CA, Guo X (2014) Overexpression of cotton GhMKK4 enhances disease susceptibility and affects abscisic acid, gibberellin and hydrogen peroxide signalling in transgenic Nicotiana benthamiana. Mol Plant Pathol 15:94–108

    Article  Google Scholar 

  • Liu Y, He J, Chen Z, Ren X, Hong X et al (2010) ABA overly-sensitive 5 (ABO5), encoding a pentatricopeptide repeat protein required for cis-splicing of mitochondrial nad2 intron 3, is involved in the abscisic acid response in Arabidopsis. Plant J 63:749–765

    Article  CAS  Google Scholar 

  • López-Bucio J, Cruz-Ramírez A, Herrera-Estrella L (2003) The role of nutrient availability in regulating root architecture. Curr Opin Plant Biol 6:280–287

    Article  Google Scholar 

  • Malamy JE (2005) Intrinsic and environmental response pathways that regulate root system architecture. Plant Cell Environ 28:67–77

    Article  CAS  Google Scholar 

  • Péret B, De Rybel B, Casimiro I, Benková E, Swarup R, Laplaz L, Beeckman T, Bennett MJ (2009a) Arabidopsis lateral root development: an emerging story. Cell 14:399–408

    Google Scholar 

  • Péret B, Larrieu A, Bennett MJ (2009b) Lateral root emergence: a difficult birth. J Exp Bot 60:3637–3643

    Article  Google Scholar 

  • Pick TR, Bräutigam A, Schulz MA, Obata T, Fernie AR, Weber AP (2013a) PLGG1, a plastidic glycolate glycerate transporter, is required for photorespiration and defines a unique class of metabolite transporters. Proc Natl Acad Sci USA 110:3185–3190

    Article  CAS  Google Scholar 

  • Pick TR, Bräutigam A, Schulz MA, Obata T, Fernie AR, Weber AP (2013b) PLGG1, a plastidic glycolate glycerate transporter, is required for photorespiration and defines a unique class of metabolite transporters. Proc Natl Acad Sci U S A 110:3185–3190

    Article  CAS  Google Scholar 

  • Sengupta D, Kannan M, Reddy AR (2011) A root proteomics-based insight reveals dynamic regulation of root proteins under progressive drought stress and recovery in Vigna radiata (L.). Planta 23:1111–1127

    Article  Google Scholar 

  • Smet De, White PJ, Bengough AG, Dupuy L, Parizot B, Casimiro I, Heidstra R, Laskowski M, Lepetit M, Hochholdinger F et al (2012) Analyzing lateral root development: how to move forward. Plant Cell 24:15–20

    Article  Google Scholar 

  • Sun L, Xu Y, Bai S, Bai X, Zhu H, Dong H, Wang W, Zhu X, Hao F, Song C (2019) Transcriptome-wide analysis of pseudouridylation of mRNA and non-coding RNAs in Arabidopsis. J Exp Bot 70(19):5089–5600

    Article  CAS  Google Scholar 

  • Vartanian N, Marcotte L, Giraudat J (1994) Drought rhizogenesis in Arabidopsis thaliana: differential responses of hormone mutants. Plant Physiol 104:761–767

    Article  CAS  Google Scholar 

  • Wasilewska A, Vlad F, Sirichandra C, Redko Y, Jammes F, Valon C, dit Frey NF, Leung J, (2008) An update on abscisic acid signaling in plants and more. Mol Plant 1(2):198–217

    Article  CAS  Google Scholar 

  • Yamaguchi M, Takechi K, Myouga F, Imura S, Sato H, Takio S, Shinozaki K, Takano H (2012) Loss of the plastid envelope protein AtLrgB causes spontaneous chlorotic cell death in Arabidopsis thalianam. Plant Cell Physiol 53:125–134

    Article  CAS  Google Scholar 

  • Yang Y, Jin H, Chen Y, Lin W, Wang C, Chen Z, Han N, Bian H, Zhu M, Wang J (2012) A chloroplast envelope membrane protein containing a putative LrgB domain related to the control of bacterial death and lysis is required for chloroplast development in Arabidopsis thaliana. New Phytol 193:81–95

    Article  CAS  Google Scholar 

  • Yin H, Zhang X, Liu J, Wang Y, He J, Yang T, Hong X, Yang Q, Gong Z (2009) Epigenetic regulation, somatic homologous recombination, and abscisic acid signaling are influenced by DNA polymerase epsilon mutation in Arabidopsis. Plant Cell 21:386–402

    Article  CAS  Google Scholar 

  • Yu F, Liu X, Alsheikh M, Park S, Rodermel S (2008) Mutations in suppressor of variegation1, a factor required for normal chloroplast translation, suppress var2-mediated leaf variegation in Arabidopsis. Plant Cell 20:1786–1804

    Article  CAS  Google Scholar 

  • Zhang J, Davies WJ (1990) Changes of ABA in xylem sap as a function of changing water status can account for changes in leaf conductance and growth. Plant Cell and Environ 13:277–285

    Article  CAS  Google Scholar 

  • Zhang H, Han W, De Smet I, Talboys P, Loya R, Hassan A, Rong H, Jürgens G, Paul Knox J, Wang MH (2010) ABA promotes quiescence of the quiescent centre and suppresses stem cell differentiation in the Arabidopsis primary root meristem. Plant J 64:764–774

    Article  CAS  Google Scholar 

  • Zuo J, Niu QW, Chua NH (2000) Technical advance: an estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants. Plant J 24:265–273

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the Ministry of Agriculture of China (2016ZX08009-003), the National Natural Science Foundation of China (31430061) and China Postdoctoral Science Foundation (2019M652521). We are grateful to Dr. Jan-Ru Zuo (Institute of Genetics and Developmental Biology, CAS, China) for providing T-DNA insertion mutant library seeds.

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Authors and Affiliations

  1. Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Institute of Biomedical Informatics, Henan University, 85 Minglun Street, Kaifeng, 475001, Henan, China

    Huan Dong, Xiaonan Ma, Xiaoli Li, Jiaxing Liu, Ling Bai & Chun-peng Song

  2. College of Life Sciences, Henan Agricultural University, Zhengzhou, 475000, Henan, China

    Huan Dong

  3. Experimental Center of Tropical Forestry, Chinese Academy of Forestry, Pingxiang, 532600, Guangxi, China

    Pei Zhang

  4. School of Marine Science, Ningbo University, Ningbo, 315211, Zhejiang, China

    Huan Wang

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  1. Huan Dong
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  2. Xiaonan Ma
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  3. Pei Zhang
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  4. Huan Wang
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  5. Xiaoli Li
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  6. Jiaxing Liu
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  7. Ling Bai
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  8. Chun-peng Song
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Contributions

CS, LB and HD conceived and directed the project. HD and HW, XL, JL, and PZ carried out all the experiments. HD, LB, and XM performed the integrated data analysis and wrote the manuscript.

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Correspondence to Ling Bai.

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Dong, H., Ma, X., Zhang, P. et al. Characterization of Arabidopsis thaliana Root-Related Mutants Reveals ABA Regulation of Plant Development and Drought Resistance. J Plant Growth Regul 39, 1393–1401 (2020). https://doi.org/10.1007/s00344-020-10076-6

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  • DOI: https://doi.org/10.1007/s00344-020-10076-6

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