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Quantification of IAA Metabolites in the Early Stages of Adventitious Rooting Might Be Predictive for Subsequent Differences in Rooting Response

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Abstract

Stock mother plants have gained importance in the process of adventitious rooting of woody plants in recent years. The present study reveals the role of the cutting position from a stock mother plant for subsequent rooting. Cuttings of Prunus subhirtella Miq. var. Autumnalis which originated from suckers at the bottom of a tree developed a rooting system of better quality (rooted cutting without callus formation, more main roots) compared to cuttings which originated from shoots at the top of a tree. The latter accumulated significantly more indole-3-acetic acid (IAA) with the highest value of 25.37 μg g−1 FW on the severance date. These cuttings also contained more indole-3-acetyl-aspartate (IAA-Asp) on the second day after severance with the highest value of 4875.95 μg g−1 FW compared to cuttings from the bottom-deriving suckers. The latter metabolised IAA primarily via 2-oxindole-3-acetic acid (oxIAA) and indole-3-methanol because the concentrations of these compounds increased at the base of these cuttings. The highest concentration of oxIAA, 8.3 mg ekv. IAA g−1 FW, was measured 1 h after severance in cuttings from the bottom-derived suckers. With 590.5 ng ekv IAAg−1 FW, the indole-3-methanol values were also significantly higher in the cuttings from the bottom shoots compared with cuttings from the top of the tree.

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Acknowledgments

The work described in this paper was funded by the Slovenian Research Agency within the national research programme Horticulture No. P4-0013-0481.

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  1. Biotechnical Faculty, Department of Agronomy, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia

    Gregor Osterc, Maja Mikulič Petkovšek & Franci Stampar

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  1. Gregor Osterc
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  2. Maja Mikulič Petkovšek
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  3. Franci Stampar
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Correspondence to Gregor Osterc.

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Osterc, G., Petkovšek, M.M. & Stampar, F. Quantification of IAA Metabolites in the Early Stages of Adventitious Rooting Might Be Predictive for Subsequent Differences in Rooting Response. J Plant Growth Regul 35, 534–542 (2016). https://doi.org/10.1007/s00344-015-9559-9

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  • DOI: https://doi.org/10.1007/s00344-015-9559-9

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