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Elevated Carbon Dioxide Induces Stomatal Closure of Arabidopsis thaliana (L.) Heynh. Through an Increased Production of Nitric Oxide

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Abstract

Elevated carbon dioxide (CO2) has been shown to enhance the photosynthesis of plants, while inducing stomatal closure in leaves. Stomatal closure is always a key issue regarding plants’ responses against stress under elevated CO2. This paper examined the mechanism underlying CO2 elevation-induced stomatal closure in Arabidopsis. Endogenous nitric oxide (NO) in guard cells was detected by the specific probe 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate. The Arabidopsis wild type, nitric oxide synthase (NOS) mutant atnos1, and nitrate reductase (NR) mutant nia1nia2 were used as experimental materials. The present study shows that elevated CO2-induced NO accumulation in guard cells by 120 % and decreased the aperture of stomata by 32 % compared with ambient CO2. CO2 elevation-induced stomatal closure was reversed by scavenging NO. Here, we suggested that NO plays an important role in stomatal closure of Arabidopsis induced by elevated CO2. The pharmacological and genetic evidence shows that both the NOS inhibitor L-NAME and NR inhibitor tungstate significantly decreased NO accumulation in guard cells and inhibited stomatal closure, whereas the stomatal aperture of both the atnos1 and nia1nia2 mutants decreased slightly under elevated CO2, indicated that both NOS-like and NR were involved in CO2 elevation-induced NO accumulation. Therefore, we conclude from these findings that elevated CO2 increases the level of NO through both NOS-like and NR, which then induces stomatal closure in Arabidopsis under elevated CO2.

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Acknowledgments

This work was financially supported by the National Key Project on Science and Technology of China [2012BAC17B02], the Chinese Ministry of Agriculture [201103004] and the Project of Scientific Emissary of Zhejiang Province [2012T2T209]. We are thankful to the 985-Institute of Agrobiology and Environmental Sciences of Zhejiang University for providing the experimental equipment.

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Authors and Affiliations

  1. Ministry of Education Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou, 310058, China

    Huan Wang, Wendan Xiao, Rushan Chai & Yongsong Zhang

  2. Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition, College of Environmental and Resource Science, Zhejiang University, Hangzhou, 310058, China

    Yaofang Niu & Chongwei Jin

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  1. Huan Wang
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  2. Wendan Xiao
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  6. Yongsong Zhang
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Correspondence to Yongsong Zhang.

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Wang, H., Xiao, W., Niu, Y. et al. Elevated Carbon Dioxide Induces Stomatal Closure of Arabidopsis thaliana (L.) Heynh. Through an Increased Production of Nitric Oxide. J Plant Growth Regul 34, 372–380 (2015). https://doi.org/10.1007/s00344-014-9473-6

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  • DOI: https://doi.org/10.1007/s00344-014-9473-6

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