Abstract
Silicon (Si) is known to be beneficial to plants in alleviating biotic and abiotic stresses. However, its uptake from soil and transport in plants is not well documented. In this work, we cloned two cucumber putative silicon transporter genes (CSiT-1 and CSiT-2) and characterized some of their functions in cucumber plants. The proteins coded by these two genes were almost identical to other plant Si influx transporters, containing two conserved NPAs (asparagine proline alanine) and four ar/R (aromatic/arginine) selectivity filter-forming residues [G(Gly)/C(Cys), S(Ser), G(Gly), and R(Arg)]. The two genes were expressed in all tissues and most interestingly the diurnal patterns of their expression were displaying a circadian rhythm. Compared with rice OsLsi1 or other plant Si transport genes, these two genes were highly expressed in leaves and their expression levels corresponded to Si supply in the hydroponic culture solution. Both CSiT-1 and CSiT-2 proteins were localized in the plasma membrane when they were heterogeneously expressed in tobacco leaves. Our data suggested that the two genes cloned from cucumber were likely coding silicon transporters and had a distinct diurnal expression pattern of a circadian rhythm both in leaves and roots.
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Bernal AJ, Jensen JK, Harholt J, Sorensen S, Moller I, Blaukopf C (2007) Disruption of ATCSLD5 results in reduced growth, reduced xylan and homogalacturonan synthase activity and altered xylan occurrence in Arabidopsis. Plant J 52:791–802
Casey WH, Kinrade SD, Knight CTG, Rains DW, Epstein E (2004) Aqueous silicate complexes in wheat, Triticum aestivum L. Plant, Cell Environ 27:51–54
Cheng BT (1982) Some significant functions of silicon to higherplants. J Plant Nutr 5:1345–1353
Chiba Y, Mitani N, Yamaji N, Ma JF (2009) HvLsi1 is a silicon influx transporter in barley. Plant J 57:810–818
Cotterill JV, Watkins RW, Brennon CB, Cowan DP (2007) Boosting silica levels in wheat leaves reduces grazing by rabbits. Pest Manag Sci 63:247–253
Epstein E (1994) The anomaly of silicon in plant biology. Proc Natl Acad Sci USA 91:11–17
Epstein E (1999) Silicon annual review of plant physiology and plant. Mol Biol 50:641–664
Fauteux F, Remus-Borel W, Menzies JG, Belanger RR (2005) Silicon and plant disease resistance against pathogenic fungi. FEMS Microbiol Lett 249:1–6
Fawe A, Menzies JG, Cherif M, Bélanger RR, Datnoff LE, Snyder GH, Korndorfer GH (2001) Silicon and disease resistance in dicotyledons Silicon in agriculture Studies in plant science. Elsevier, Amsterdam, pp 159–169
Guntzer F, Keller C, Meunier J (2012) Benefits of plant silicon for crops a review. Agron Sustain Dev 32:201–213
Johansen LK, Carrington JC (2001) Silencing on the spot. Induction and suppression of RNA silencing in the agrobacterium-mediated transient expression system. Plant Physiol 126:930–938
Liang YC, Sun WC, Zhu YG, Christie P (2007) Mechanisms of silicon-mediated alleviation of abiotic stresses in higher plants a review. Environ Pollut 147:422–428
Liu QP, Wang HS, Zhang ZH, Wu JS, Feng Y (2009) Divergence in function and expression of the NOD26-like intrinsic proteins in plants. BMC Genom 10:313
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using eal-time quantitative PCR and the 2−ΔΔCT method. Methods 25:402–408
Ma JF (2010) Si transporters in higher plant. In: Jhon PT, Bienert PG (eds) MIPs and their role in the exchange of materials. Landes Bioscience, Austin, pp 99–109
Ma JF, Yamaji N (2006) Silicon uptake and accumulation in higher plants. Trends Plant Sci 11:392–397
Ma JF, Tamai K, Ichii M, Wu GF (2002) A rice mutant defective in Si uptake. Plant Physiol 130:2111–2117
Ma JF, Mitani N, Nagao S, Konishi S, Tamai K, Iwashita T, Yano M (2004) Characterization of the silicon uptake and molecular mapping of the silicon transporter gene in rice. Plant Physiol 136:3284–3289
Ma JF, Tamai K, Yamaji N, Mitani N, Konishi S, Katsuhara M, Ishiguro M, Murata Y, Yano M (2006) A silicon transporter in rice. Nature 440:688–691
Ma JF, Yamaji N, Mitani N, Tamai K, Konishi S, Fujiwara T, Katsuhara M, Yano M (2007) An efflux transporter of silicon in rice. Nature 448:209–212
Mitani N, Ma JF (2005) Uptake system of silicon in different plant species. J Exp Bot 56(414):1255–1261
Mitani N, Chiba Y, Yamaji N, Ma JF (2009) Identification and characterization of maize and barley Lsi2-like silicon efflux transporters reveals a distinct silicon uptake system from that in rice. Plant Cell 21:2133–2142
Montpetit J, Vivancos J, Mitani-Ueno N, Yamaji N, Rémus-Borel W, Belzile F, Ma JF, Bélanger RR (2012) Cloning, functional characterization and heterologous expression of TaLsi1, a wheat silicon transporter gene. Plant Mol Biol 79:35–46
Sangster AG, Hodson MJ, Tubb HJ, Datnoff LE, Snyder GH, Korndorfer GH (2001) Silicon deposition in higher plants Silicon in agriculture, Studies in plant science. Elsevier, Amsterdam, pp 85–113
Savant NK, Snyder GH, Datnoff LE (1997) Silicon management and sustainable rice production. Adv Agron 58:151–199
Sposito G (1989) The chemistry of soils. Oxford University Press, New York, p 277
Takahashi K, Ma JF, Miyake Y (1990) The possibility of silicon as an essential element for higher plants. Comments Agric Food Chem 2:99–122
Xue WY, Xing YZ, Weng XY, Zhao Y, Tang WJ, Wang L, Zhou HJ, Yu SB, Xu CG, Li XH, Zhang QF (2008) Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nat Genet 40:761–767
Yamaji N, Ma JF (2009) Silicon transporter Lsi6 at the node is responsible for inter-vascular transfer of silicon in rice. Plant Cell 21:2878–2883
Yamaji N, Mitatni N, Ma JF (2008) A transporter regulating silicon distribution in rice shoots. Plant Cell 20:1381–1389
Zhang C, Wang L, Nie Q, Zhang W, Zhang F, Oryza sativa L (2008) Long-term effects of exogenous silicon on cadmium translocation and toxicity in rice. Environ Exp Bot 62:300–307
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This work was supported by grants from the National Natural Science Foundation of China (31101554) and the Natural Science Foundation of Zhejiang Province (Y3110340 and LQ12C15002).
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Hua-Sen Wang and Chao Yu have contributed equally to this work.
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Wang, HS., Yu, C., Fan, PP. et al. Identification of Two Cucumber Putative Silicon Transporter Genes in Cucumis sativus . J Plant Growth Regul 34, 332–338 (2015). https://doi.org/10.1007/s00344-014-9466-5
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DOI: https://doi.org/10.1007/s00344-014-9466-5