Abstract
In periwinkle cell suspensions, the accurate quantification of gene expression through real-time RT-PCR showed that two type-A response regulators (RR-A), considered primary cytokinin (CK)-responsive genes, were differentially regulated after CK treatment. Specific inhibition of phospholipase D (PLD)-dependent phosphatidic acid (PA) production by primary alcohols reduced significantly the transcript level of one gene in response to CK, although the other gene was unaffected. Moreover, this inhibitory effect on gene transcript level could be antagonized by exogenous supply of PA. These results suggest that PA, likely released from the membrane by PLD activity, could operate in the early steps of CK signalling in periwinkle cells.
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Acknowledgments
This research was supported by the Ministère de l’Education Nationale et de la Technologie (MENRT, France). A doctoral grant was awarded by the MENRT to AA.
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344_2008_9058_MOESM1_ESM.eps
Fig. S1 Effect of of phosphatidic acid (PA) and linoleic acid (LA) on CrRR1 transcript level in CK-treated cells under PLD inhibition. Three-day-old cell cultures were pre-incubated for 30 min with 1% butan-1-ol or not. The cells were subsequently treated for 30 min with 5 µM BA (CK) alone or in combination with 25, 50, 100 µM PA; 50 µM linoleic acid (LA). Mean values ± SE, (n = 3). Letters indicate significant differences at P < 0.05 level (EPS 996 kb)
344_2008_9058_MOESM2_ESM.eps
Fig. S2 Effect of of phosphatidic acid (PA) and linoleic acid (LA) on CrRR1 transcript level under standard conditions. Three-day-old cell cultures were treated for 30 min with 25, 50, 100 µM PA; 50 µM linoleic acid (LA); 5 µM BA (CK; as a control of transcription under CK) or untreated (control). Mean values ± SE, (n = 3). Letters indicate significant differences at P < 0.05 level (EPS 829 kb)
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Amini, A., Glévarec, G., Andreu, F. et al. Effects of Phosphatidic Acid on Cytokinin Signal Transduction in Periwinkle Cells. J Plant Growth Regul 27, 394–399 (2008). https://doi.org/10.1007/s00344-008-9058-3
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DOI: https://doi.org/10.1007/s00344-008-9058-3