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Genome-wide expression profiling of ARABIDOPSIS RESPONSE REGULATOR 7(ARR7) overexpression in cytokinin response

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Abstract

The type-A ARRs of cytokinin two-component signaling system act as negative regulators for cytokinin signaling except for ARR4, but the molecular mechanism by which the A-type ARRs regulate cytokinin signaling remain elusive. To get insights into the molecular function of A-type ARR in cytokinin response, we sought to find the components that function downstream of A-type ARR protein by investigating the effects of ARR7 overexpression on cytokinin-regulated gene expression with the Affymetrix full genome array. To examine early cytokinin response, plants were treated with cytokinin for 30 min or 2 h, followed by GeneChip analysis. The hierarchical clustering analysis of our GeneChip data showed that ARR7 overexpression had distinctively repressive impacts on various groups of the cytokinin-regulated genes. In particular, the induction of all A-type ARRs except for ARR22, and AHK(ARABIDOPSIS HISTIDINE KINASE)1 and AHK4 was suppressed by ARR7. Cytokinin-induced expression of most of 12 expansin genes were repressed by ARR7, indicating potential involvement of ARR7 in cell expansion and plant development. Up-regulation of five cytokinin oxidase genes by cytokinins was negatively affected by ARR7. Our GeneChip analysis suggest that ARR7 mainly acts as a transcriptional repressor for a variety of early cytokinin-regulated genes encoding transcription factors, signal transmitters, plant development, and cellular metabolism, which may be responsible for reduced sensitivity of Arabidopsis transgenic plants overexpressing ARR7 to exogenous cytokinins.

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Acknowledgments

This work was supported by grants from the Plant Diversity Research Center of 21st Century Frontier Research Program (PF0330404-02), funded by the Ministry of Science and Technology of Korean government and from the Agricultural Plant Stress Research Center (R11-20010003101-0) funded by Korea Science and Engineering Foundation to J. Kim. We thank Seoulin Bioscience for technical assistance with GeneChip analysis.

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  1. Department of Plant Biotechnology and Agricultural Plant Stress Research Center, Chonnam National University, Puk-Gu, Gwangju, 500-757, South Korea

    Dong Ju Lee, Jin-Young Park, Su-Jin Ku, Young-Min Ha, Sunmi Kim, Myung Duk Kim, Man-Ho Oh & Jungmook Kim

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  1. Dong Ju Lee
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  2. Jin-Young Park
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Correspondence to Jungmook Kim.

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Communicated by S. Hohmann.

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Lee, D.J., Park, JY., Ku, SJ. et al. Genome-wide expression profiling of ARABIDOPSIS RESPONSE REGULATOR 7(ARR7) overexpression in cytokinin response. Mol Genet Genomics 277, 115–137 (2007). https://doi.org/10.1007/s00438-006-0177-x

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