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Exogenous Catalase and Ascorbate Modify the Effects of Abscisic Acid (ABA) on Root Hydraulic Properties in Phaseolus vulgaris L. Plants

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Abstract

Abscisic acid (ABA) modifies the hydraulic properties of roots by increasing root water flux (Jv). The role of reactive oxygen species (ROS) in this ABA-induced process was evaluated. At the same time, some antioxidant enzyme activities in root tissues were measured. Phaseolus vulgaris plants were grown hydroponically, and different concentrations of ABA in combination with catalase enzyme or ascorbate were added to the nutrient solution. Catalase treatment had no effect by itself (no ABA) and had little or only a small stimulatory effect at ABA concentrations of 1, 50, and 100 μM, but it partially inhibited the ABA effect at 5 μM. Ascorbate by itself doubled Jv and root hydraulic conductance over the control value. In the presence of ABA, ascorbate partially or, at 100 μM, completely inhibited that ABA stimulation of Jv. These results are discussed in relationship to the possibility that ABA signaling in the roots involves ROS.

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References

  • Aebi H. 1984. Catalase in vitro. Methods Enzymol 105:121–126

    CAS  PubMed  Google Scholar 

  • Aroca R, Amodeo G, Fernández-Illescas S, Herman EM, Chaumont F, et al. 2005. The role of aquaporins and membrane damage in chilling and hydrogen peroxide induce changes in the hydraulic conductance of maize roots. Plant Physiol 137:341–353

    Article  CAS  PubMed  Google Scholar 

  • Aroca R, Irigoyen JJ, Sánchez-Díaz M. 2001. Photosynthetic characteristics and protective mechanisms against oxidative stress during chilling and subsequent recovery in two maize varieties differing in chilling sensitivity. Plant Sci 161:719–726

    Article  CAS  Google Scholar 

  • Aroca R, Vernieri P, Irigoyen JJ, Sánchez-Díaz M, Tognoni F, et al. 2003. Involvement of abscisic acid in leaf and root of maize (Zea mays L.) in avoiding chilling-induced water stress. Plant Sci 165:671–679

    Article  CAS  Google Scholar 

  • BassiriRad H, Radin JW. 1992. Temperature-dependent water and ion transport properties of barley and sorghum roots. Plant Physiol 99:34–37

    CAS  Google Scholar 

  • Chinnusany V, Schumaker K, Zhu J-K. 2004. Molecular genetics perspectives on crosstalk and specificity in abiotic stress signalling in plants. J Exp Bot 55:225–236

    Google Scholar 

  • Collins JC, Morgan M. 1980. The influence of temperature on the abscisic acid stimulated water flow from excised maize roots. New Phytol 84:19–26

    CAS  Google Scholar 

  • Cross JB, Currier RP, Torraco DJ, Vanderberg LA, Wagner GL, et al. 2003. Killing of Bacillus spores by aqueous dissolved oxygen, ascorbic acid, and copper ions. Appl Environ Microbiol 69:2245–2252

    Article  CAS  PubMed  Google Scholar 

  • Desikan R, Cheung M-K, Bright J, Henson D, Hancock JT, et al. 2004. ABA, hydrogen peroxide and nitric oxide signalling in stomatal guard cells. J Exp Bot 55:205–212

    CAS  PubMed  Google Scholar 

  • Fan S, Blake TJ. 1994. Abscisic acid induced electrolyte leakage in woody species with contrasting ecological requirements. Physiol Plant 90:414–419

    CAS  Google Scholar 

  • Fernández-García N, Martínez V, Cerdá A, Carvajal M. 2002. Water and nutrient uptake of grafted tomato plants grown under saline conditions. J Plant Physiol 159:899–905

    Google Scholar 

  • Foyer CH, Noctor G. 2005. Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in physiological context. Plant Cell Environ 28:1056–1071

    Article  CAS  Google Scholar 

  • Henzler T, Waterhouse RN, Smith AJ, Carvajal M, Cooke DT, et al. 1999. Diurnal variations in hydraulic conductivity and root pressure can be correlated with the expression of putative aquaporins in the roots of Lotus japonicus. Planta 210:50–60

    Article  CAS  PubMed  Google Scholar 

  • Henzler T, Ye Q, Steudle E. 2004. Oxidative gating of water channels (aquaporins) in Chara by hydroxyl radicals. Plant Cell Environ 27:1184–1195

    Article  CAS  Google Scholar 

  • Hose E, Steudle E, Hartung W. 2000. Abscisic acid and hydraulic conductivity of maize roots: a study using cell- and root-pressure probes. Planta 211:874–882

    Article  CAS  PubMed  Google Scholar 

  • Jiang M, Zhang J. 2001. Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol 42:1265–1273

    Article  CAS  PubMed  Google Scholar 

  • Jiang M, Zhang J. 2002. Involvement of plasma-membrane NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense in leaves of maize seedlings. Planta 215:1022–1030

    Article  CAS  PubMed  Google Scholar 

  • Köhler B, Hills A, Blatt MR. 2003. Control of guard cell ion channels by hydrogen peroxide and abscisic acid indicates their action through alternate signaling pathways. Plant Physiol 131:385–388

    PubMed  Google Scholar 

  • Ktitorova IN, Skobeleva OV, Sharova EI, Ermakov EL. 2002. Hydrogen peroxide appears to mediate a decrease in hydraulic conductivity in wheat roots under salt stress [in Russian]. J Plant Physiol 49:369–380

    CAS  Google Scholar 

  • Lee SH, Singh AP, Chung GC. 2004. Rapid accumulation of hydrogen peroxide in cucumber roots due to exposure to low temperature appears to mediate decrease in water transport. J Exp Bot 55:1733–1741

    Article  CAS  PubMed  Google Scholar 

  • Lin CC, Kao CH. 2001. Abscisic acid induced changes in cell wall peroxidase activity and hydrogen peroxide level in roots of rice seedlings. Plant Sci 160:323–329

    Article  CAS  PubMed  Google Scholar 

  • Ludewig M, Dörffling K, Seifert H. 1988. Abscisic acid and water transport in sunflowers. Planta 175:325–333

    Article  CAS  Google Scholar 

  • Luu DT, Maurel C. 2005. Aquaporins in a challenging environment: molecular gears for adjusting plant water status. Plant Cell Environ 28:85–96

    Article  CAS  Google Scholar 

  • Markhart AH III, Fiscus EL, Naylor AW, Kramer PJ. 1979. Effect of abscisic acid on root hydraulic conductivity. Plant Physiol 64:611–614

    CAS  Google Scholar 

  • Mittler R, Vanderauwera S, Gollery M, Van Breusegem F. 2004. Reactive oxygen gene network of plants. Trends Plant Sci 9:490–498

    Article  CAS  PubMed  Google Scholar 

  • Mori IC, Schroeder JI. 2004. Reactive oxygen species activation of plant Ca2+ channels. A signaling mechanism in polar growth, hormone transduction, stress signaling, and hypothetical mechanotransduction. Plant Physiol 135:702–708

    Article  CAS  PubMed  Google Scholar 

  • Noctor G, Foyer CH. 1998. Ascorbate and glutathione: keeping active oxygen species under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279

    Article  CAS  PubMed  Google Scholar 

  • Pignocchi C, Foyer CH. 2003. Apoplastic ascorbate metabolism and its role in the regulation of cell signalling. Curr Opin Plant Biol 6:379–389

    Article  CAS  PubMed  Google Scholar 

  • Schraut D, Heilmeier H, Hartung W. 2005. Radial transport of water and abscisic acid (ABA) in roots of Zea mays under conditions of nutrient deficiency. J Exp Bot 56:879–886

    Article  CAS  PubMed  Google Scholar 

  • Tomos AD, Leigh RA. 1999. The pressure probe: a versatile tool in plant cell physiology. Annu Rev Plant Physiol Plant Mol Biol 50:447–472

    Article  CAS  PubMed  Google Scholar 

  • Tsai Y-C, Kao CH. 2004. The involvement of hydrogen peroxide in abscisic acid-induced activities of ascorbate peroxidase and glutathione reductase in rice roots. J Plant Growth Regul 43:207–212

    Article  CAS  Google Scholar 

  • Van Breusegem F, Vranová E, Dat JF, Inzé D. 2001. The role of active oxygen species in plant signal transduction. Plant Sci 161:405–414

    Google Scholar 

  • Vysotskaya LB, Arkhipova TN, Timergalina LN, Dedov AV, Veselov SY, et al. 2004. Effect of partial root excision on transpiration, root hydraulic conductance and leaf growth in wheat seedlings. Plant Physiol Biochem 42:251–255

    Article  CAS  PubMed  Google Scholar 

  • Wan X, Zwiazek JJ. 2001. Root water flow and leaf stomatal conductance in aspen (Populus tremuloides) seedlings treated with abscisic acid. Planta 213:741–747

    Article  CAS  PubMed  Google Scholar 

  • Zhang J, Zhang X, Liang J. 1995. Exudation rate and hydraulic conductivity of maize roots are enhanced by soil drying and abscisic acid treatment. New Phytol 131:329–336

    CAS  Google Scholar 

  • Zhang X, Zhang L, Dong F, Gao J, Galbraith DW, et al. 2001. Hydrogen peroxide is involved in abscisic acid-induced stomatal closure in Vicia faba. Plant Physiol 126:1438–1448

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The author thanks Prof. M. J. Chrispeels (University of California San Diego) for making his laboratory facilities available for these experiments, as well as for stimulating discussions and for editing the manuscript. The author was supported by a postdoctoral fellowship from the Ministerio de Educación y Ciencia (Spain). The author is also grateful to Dr. M. J. Martín for help during the experiments.

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  1. Division of Biological Sciences, University of California San Diego, 9500 Gilman Drive, La Jolla, California, 92093-0116, USA

    Ricardo Aroca

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  1. Ricardo Aroca
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Correspondence to Ricardo Aroca.

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Aroca, R. Exogenous Catalase and Ascorbate Modify the Effects of Abscisic Acid (ABA) on Root Hydraulic Properties in Phaseolus vulgaris L. Plants. J Plant Growth Regul 25, 10–17 (2006). https://doi.org/10.1007/s00344-005-0075-1

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  • DOI: https://doi.org/10.1007/s00344-005-0075-1

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