Abstract
Dinoflagellates are the major causative agents of harmful algal blooms in the global ocean and they usually form blooms under conditions of very low dissolved inorganic phosphorus (DIP). However, the mechanisms underpinning the dinoflagellate blooms remain unclear. Here, we quantitatively compared protein expression profiles of a marine dinoflagellate, Prorocentrum donghaiense, grown in inorganic P-replete, P-deficient, and DIP- and dissolved organic phosphorus (DOP)-resupplied conditions by employing a Tandem Mass Tag (TMT)-based quantitative proteomic approach. Proteins involved in intracellular P reallocation, organic P, and non-P lipid utilization were up-regulated under the P-deficient condition, while inorganic phosphate transporters varied insignificantly. In response to the P resupplementation, nitrogen metabolism, ribosome, porphyrin, and chlorophyll metabolism were up-regulated, while lysosome, and starch and sucrose metabolism were down-regulated. Notably, photosynthesis was up-regulated and secondary metabolism was down-regulated only in the DIP-resupplied cells, whereas amino acid metabolism and vitamin B6 metabolism were up-regulated in the DOP-resupplied cells, indicating differential response mechanisms of P. donghaiense to DIP or DOP resupplementation. Our results indicated that P. donghaiense initiated multiple strategies in response to an ambient inorganic P-deficiency, and its efficient DOP assimilation by providing both P and carbon sources might be a key factor driving bloom formations of P. donghaiense in a low DIP environment.
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Data Availability Statement
The datasets that support the findings of this study are available from the corresponding author on reasonable request.
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Supported by the National Key Research Development Program of China (No. 2017YFC1404302), the National Natural Science Foundation of China (Nos. 41425021, 41706131), and the Open Fund of CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences (No. KLMEES201806). Dazhi WANG was also supported by the “Ten-Thousand Talents Program” for leading talents in science and technological innovation
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Zhang, S., Yuan, C., Chen, Y. et al. Quantitative proteomics provides insight into the response of the marine dinoflagellate Prorocentrum donghaiense to changes in ambient phosphorus. J. Ocean. Limnol. 40, 563–576 (2022). https://doi.org/10.1007/s00343-021-1030-0
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DOI: https://doi.org/10.1007/s00343-021-1030-0